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PTP pi/PTPRU Antibody (764209)

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Western blot shows lysates of human brain (cerebellum) tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PTPp/PTPRU Monoclonal Antibody (Catalog # MAB7475) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PTPp/PTPRU at approximately 180-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain (cerebellum) tissue and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PTPp/PTPRU Monoclonal Antibody (Catalog # MAB7475) followed by ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB
Clone
764209
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7475-SP
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PTP pi/PTPRU Antibody (764209) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human PTPπ/PTPRU
Glu19-Gln740
Accession # Q92729
Specificity
Detects human and rat PTPπ/PTPRU in Western blots and recombinant human PTPRU in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PTPRK, rhPTPRM, or rhPTPRT is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTPRU
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB7475 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTP pi/PTPRU Antibody (764209)

  • FMI
  • GLEPP1
  • hPTP-J
  • pancreatic carcinoma phosphatase 2
  • PCP2
  • PCP-2
  • pi R-PTP-Psi
  • protein tyrosine phosphatase J
  • protein tyrosine phosphatase, receptor type, U
  • protein-tyrosine phosphatase J
  • protein-tyrosine phosphatase pi
  • PTP pi
  • PTP
  • PTP-J
  • PTP-PI
  • PTPPSI
  • PTPRO
  • PTP-RO
  • PTPRU
  • Receptor protein tyrosine phosphatase hPTP-J
  • R-PTP-PSI
  • R-PTP-U

Background

Protein Tyrosine Phosphatase, receptor type U (PTPRU), also called PCP-2 or PTPp, is an approximately 180 kDa type I transmembrane glycoprotein that is a type IIb receptor-like protein tyrosine phosphatase (PTPR). The 731 amino acid (aa) extracellular domain contains MAM, Ig-like C-type and Fibronectin III domains that often mediate cell adhesion, but unlike other family members, PTPRU does not appear to mediate homophilic cell aggregation. It does, however, antagonize Wnt signaling in adherens junctions by inhibiting beta -catenin-mediated gene transcription. Human PTPRU shares 95% aa sequence identity with mouse and rat PTPRU within the extracellular domain, and up to 60% aa identity with other human family members. Isoforms of 1436, 1440 and 1433 aa are altered within the cytoplasmic domain as compared to the 1446 aa full-length form. Several tissues contain detectable amounts of PTPRU mRNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTP pi/PTPRU Antibody (MAB7475)(4)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ELISA Detection.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PTPRU
Uniprot