STEP Antibody (23E5)

STEP (STriatal-Enriched protein tyrosine Phosphatase; PTPN5) is a brain-specific phosphatase enzyme which is regarded as an "LTD protein" because it mediates AMPA receptor internalization during metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD). STEP regulates MAPKs, Src kinases and NMDA receptors which are involved in synaptic plasticity as well as in neuronal cell survival mechanisms. STEP is highly expressed in striatum and is also found in hippocampus, cortex, amygdala, optic nerve, and spinal cord, but not expressed in cerebellum. PKA mediated phosphorylation at Ser-245 site deactivates STEP whereas MAPKs regulated phosphorylation at Thr-255/Ser-268 stabilizes STEP through preventing its proteasomal degradation. Out of four alternatively spliced variants (STEP61, STEP46, STEP38, STEP20), two major isoforms namely STEP61 and STEP46, contains a signature consensus tyrosine phosphatase sequence, [I/V]HCxAGxxR[S/T]G, necessary for its catalytic activity, and a kinase-interacting motif (KIM) essential for substrate binding. During extrasynaptic NMDAR stimulation, a truncated STEP product, STEP33, is generated by calpain-mediated cleavage between residues Ser224 and Leu225 in the KIM domain leading to disruption of STEP's ability to associate with and dephosphorylate its substrates. Subsequently, proteolytic cleavage of STEP after extrasynaptic NMDAR stimulation results in p38 activation, and initiates cell death signaling cascade. STEP participates in dopaminergic, glutamatergic, and nicotinic signaling, thereby playing a key role in psychostimulant addiction and Alzheimer's disease.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-Fr, IP, WB.

Showing Publications 1 - 10 of 39. Show All 39 Publications. Collapse Publications.

Publications using NB300-202	Applications	Species
Wang C, Jiang Q, Zhao P Sevoflurane exposure during the second trimester induces neurotoxicity in offspring rats by hyperactivation of PARP-1 Psychopharmacology 2022-07-20 [PMID: 35859039] (WB, Rat)	WB	Rat
Rajagopal S, Poddar R, Paul S Tyrosine phosphatase STEP is a key regulator of glutamate-induced prostaglandin E2 release from neurons The Journal of biological chemistry 2021-07-08 [PMID: 34246631] (WB)	WB
Rajagopal S, Yang C, DeMars KM, et al. Regulation of post-ischemic inflammatory response: A novel function of the neuronal tyrosine phosphatase STEP Brain, behavior, and immunity 2021-01-08 [PMID: 33422638] (WB, Mouse)	WB	Mouse
Wang Y, Liu Y, Bi X, Baudry M Calpain-1 and Calpain-2 in the Brain: New Evidence for a Critical Role of Calpain-2 in Neuronal Death Cells 2020-12-16 [PMID: 33339205] (WB)	WB
Hu P, Maita I, Phan ML et al. Early-life stress alters affective behaviors in adult mice through persistent activation of CRH-BDNF signaling in the oval bed nucleus of the stria terminalis Transl Psychiatry 2020-11-11 [PMID: 33177511] (IF/IHC, Mouse)	IF/IHC	Mouse
Huang L, Peng Z, Lu C et al. Ginsenoside Rg1 alleviates repeated alcohol exposure-induced psychomotor and cognitive deficits Chin Med 2020-05-07 [PMID: 32411290] (WB, Mouse)	WB	Mouse
Hu P, Liu J, Maita I et al. Chronic Stress Induces Maladaptive Behaviors by Activating Corticotropin-Releasing Hormone Signaling in the Mouse Oval Bed Nucleus of the Stria Terminalis J. Neurosci. 2020-02-11 [PMID: 32054675] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Selective reduction of APP-BACE1 activity improves memory via NMDA NR2B receptor-mediated mechanisms in aged PDAPP mice. Evans C, Thomas R, Freeman T et al. Neurobiol Aging [PMID: 30572184] (WB, Mouse)	WB	Mouse
Carvajal FJ, Mira RG, Rovegno M et al. Age-related NMDA signaling alterations in SOD2 deficient mice. Biochim. Biophys. Acta. 2018-03-22 [PMID: 29577983] (ICC/IF)	ICC/IF
Pose-Utrilla J, Garcia-Guerra L, Del Puerto A et al. Excitotoxic inactivation of constitutive oxidative stress detoxification pathway in neurons can be rescued by PKD1. Nat Commun 2017-12-22 [PMID: 29273751] (Mouse)		Mouse
Won S, Incontro S, Nicoll RA, Roche KW. PSD-95 stabilizes NMDA receptors by inducing the degradation of STEP61 Proc. Natl. Acad. Sci. U.S.A. 2016-07-25 [PMID: 27457929] (WB)	WB
Rajagopal S, Deb I, Poddar R, Paul S. Aging is associated with dimerization and inactivation of the brain-enriched tyrosine phosphatase STEP. Neurobiology of Aging. 2016-02-12 [PMID: 27103516] (IP, Rat)	IP	Rat
Poddar R, Rajagopal S, Shuttleworth CW, Paul S. Zn2+-dependent activation of the Trk signaling pathway induces phosphorylation of the brain-enriched tyrosine phosphatase STEP: molecular basis for Zn2+-induced ERK MAPK activation. J. Biol. Chem. 2015-11-16 [PMID: 26574547] (IP, WB, Rat)	IP, WB	Rat
Kim HN, Jang JY, Choi BT. A single fraction from Uncaria sinensis exerts neuroprotective effects against glutamate-induced neurotoxicity in primary cultured cortical neurons Anat Cell Biol 2015-06-01 [PMID: 26140220] (WB, Rat) Details: STEP antibody was used for WB assays on rat's primary cortical neurons which were treated with hexane extract from Uncaria sinensis (JGH43IA 0.1 ug/ml) for 24 hours followed by 300uM of glutamate for 15 minutes (Fig. 5 ).	WB	Rat
Wang Y, Briz V, Chishti A et al. Distinct Roles for u-Calpain and m-Calpain in Synaptic NMDAR-Mediated Neuroprotection and Extrasynaptic NMDAR-Mediated Neurodegeneration. J Neurosci. 2013-11-27 [PMID: 24285894] (WB, Rat)	WB	Rat
Deb I, Manhas N, Poddar R et al. Neuroprotective Role of a Brain-Enriched Tyrosine Phosphatase, STEP, in Focal Cerebral Ischemia. J Neurosci. 2013-11-06 [PMID: 24198371] (IP, WB, Rat)	IP, WB	Rat
Kim HN, Kim YR, Jang JY et al. Neuroprotective effects of Polygonum multiflorum extract against glutamate-induced oxidative toxicity in HT22 hippocampal cells. J Ethnopharmacol. 2013-08-22 [PMID: 23973786] (WB, Mouse)	WB	Mouse
Sun WL, Zelek-Molik A, McGinty JFet al. Short and long access to cocaine self-administration activates tyrosine phosphatase STEP and attenuates GluN expression but differentially regulates GluA expression in the prefrontal cortex. Psychopharmacology (Berl) 2013-04-27 [PMID: 23624776] (IP, Rat)	IP	Rat
Shinohara Y, Hosoya A, Yahagi K, Ferecsko AS, Yaguchi K, Sik A, Itakura M, Takahashi M, Hirase H. Hippocampal CA3 and CA2 have distinct bilateral innervation patterns to CA1 in rodents. Eur J Neurosci;35(5):702-710. doi: 10.1111/j.1460-9568.2012.07993.x. 2012-03-01 [PMID: 22339771]
Farooq M, Kaswala RH, Kleiman NJ, Kasinathan C, Frederikse PH. GluA2 AMPA glutamate receptor subunit exhibits codon 607 Q/R RNA editing in the lens. Biochem Biophys Res Commun;418(2):273-7. 2012-02-10 [PMID: 22266371]
Davis MM, Olausson P, Greengard P, Taylor JR, Nairn AC. Regulator of calmodulin signaling knockout mice display anxiety-like behavior and motivational deficits. Eur J Neurosci;35(2):300-8. 2012-01-01 [PMID: 22250817] (WB, Mouse)	WB	Mouse
Mukherjee S, Poddar R, Deb I, Paul S. Dephosphorylation of specific sites in the KIS domain leads to ubiquitin-mediated degradation of the tyrosine phosphatase STEP. Biochem J. 2011-07-21 [PMID: 21777200]
Poddar R, Deb I, Mukherjee S, Paul S. NR2B-NMDA receptor mediated modulation of the tyrosine phosphatase STEP regulates glutamate induced neuronal cell death. J Neurochem 2010-12-01 [PMID: 21029094] (WB, Rat)	WB	Rat
Zhang Y, Kurup P, Xu J et al. Genetic reduction of striatal-enriched tyrosine phosphatase (STEP) reverses cognitive and cellular deficits in an Alzheimer's disease mouse model. Proc Natl Acad Sci U S A;107(44):19014-9. 2010-11-02 [PMID: 20956308] (WB, IP, Mouse)	WB, IP	Mouse
Kurup P, Zhang Y, Xu J, Venkitaramani DV, Haroutunian V, Greengard P, Nairn AC, Lombroso PJ. Abeta-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61. J Neurosci;30(17):5948-57. 2010-04-28 [PMID: 20427654] (WB)	WB
DiRocco DP, Scheiner ZS, Sindreu CB et al. A Role for Calmodulin-Stimulated Adenylyl Cyclases in Cocaine Sensitization. J Neurosci;29(8):2393-403. 2009-02-25 [PMID: 19244515] (ICC/IF, Mouse)	ICC/IF	Mouse
Goebel-Goody SM, Davies KD, Alvestad Linger RM, Freund RK, Browning MD. Phospho-regulation of synaptic and extrasynaptic N-methyl-d-aspartate receptors in adult hippocampal slices. Neuroscience;158(4):1446-59. 2009-02-18 [PMID: 19041929]
Zhang Y, Venkitaramani DV, Gladding CM, Zhang Y, Kurup P, Molnar E, Collingridge GL, Lombroso PJ. The tyrosine phosphatase STEP mediates AMPA receptor endocytosis after metabotropic glutamate receptor stimulation. J Neurosci;28(42):10561-6. 2008-10-15 [PMID: 18923032] (IF/IHC, WB, Mouse, Rat)	IF/IHC, WB	Mouse, Rat
Zhao, M et al. Synaptic plasticity (and the lack thereof) in hippocampal CA2 neurons. J Neurosci;27(44):12025-32. 2007-10-31 [PMID: 17978044]
Hu Y, Zhang Y, Venkitaramani DV, Lombroso PJ. Translation of striatal-enriched protein tyrosine phosphatase (STEP) after beta1-adrenergic receptor stimulation. J Neurochem;103(2):531-41. 2007-10-01 [PMID: 17623046] (IHC-Fr, WB, Rat)	IHC-Fr, WB	Rat
Choi, YS et al. Status epilepticus-induced somatostatinergic hilar interneuron degeneration is regulated by striatal enriched protein tyrosine phosphatase. J Neurosci;27(11):2999-3009. 2007-03-14 [PMID: 17360923] (IF/IHC, Mouse)	IF/IHC	Mouse
Paul S, Nairn AC, Wang P, Lombroso PJ. NMDA-mediated activation of the tyrosine phosphatase STEP regulates the duration of ERK signaling. Nat Neurosci;6(1):34-42. 2003-01-01 [PMID: 12483215] (IP, WB)	IP, WB
Nguyen TH, Liu J, Lombroso PJ. Striatal enriched phosphatase 61 dephosphorylates Fyn at phosphotyrosine 420. J Biol Chem;277(27):24274-9. 2002-07-05 [PMID: 11983687] (WB)	WB
Pelkey KA, Askalan R, Paul S, Kalia LV, Nguyen TH, Pitcher GM, Salter MW, Lombroso. Tyrosine phosphatase STEP is a tonic brake on induction of long-term potentiation. Neuron. 34(1):127-38. 2002-03-28 [PMID: 11931747]
Paul S, Snyder GL, Yokakura H, Picciotto MR, Nairn AC, Lombroso PJ. The Dopamine/D1 receptor mediates the phosphorylation and inactivation of the protein tyrosine phosphatase STEP via a PKA-dependent pathway. J Neurosci;20(15):5630-8. 2000-08-01 [PMID: 10908600] (WB, IP, Rat)	WB, IP	Rat
Nguyen TH, Paul S, Xu Y, Gurd JW, Lombroso PJ. Calcium-dependent cleavage of striatal enriched tyrosine phosphatase (STEP). J Neurochem;73(5):1995-2001. 1999-11-01 [PMID: 10537058]
Gurd JW, Bissoon N, Nguyen TH, Lombroso PJ, Rider CC, Beesley PW, Vannucci SJ. Hypoxia-ischemia in perinatal rat brain induces the formation of a low molecular weight isoform of striatal enriched tyrosine phosphatase (STEP). J Neurochem;73(5):1990-4. 1999-11-01 [PMID: 10537057]
Bult A, Zhao F, Dirkx R Jr, Sharma E, Lukacsi E, Solimena M, Naegele JR, Lombroso PJ. STEP61: a member of a family of brain-enriched PTPs is localized to the endoplasmic reticulum. J Neurosci;16(24):7821-31. 1996-12-15 [PMID: 8987810] (IHC-Fr, WB, Rat)	IHC-Fr, WB	Rat
Boulanger LM, Lombroso PJ, Raghunathan A, During MJ, Wahle P, Naegele JR. Cellular and molecular characterization of a brain-enriched protein tyrosine phosphatase. J Neurosci;15(2):1532-44. 1995-02-01 [PMID: 7869116]
Show All 39 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	WB	Mouse	12/23/2014	View Summary Application Western Blot Sample Tested See PMID 22266371 Species Mouse

Array NB300-202

• STEP Antibodies
• STEP Lysates
• STEP Proteins