![Western Blot: Smad7 Antibody - Azide Free [NB100-56688] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Smad7-Antibody---Azide-Free-Western-Blot-NB100-56688-img0004.jpg "Western Blot: Smad7 Antibody - Azide Free [NB100-56688] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody. Image using the standard format of this product.")
| Reactivity | Hu, Mu, Sh, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide Free |
| Concentration | 1 mg/ml |
| Immunogen | This antibody was raised against two peptides made from within amino acids 10 and 50 of human SMAD7. This sequence is 100% or highly conserved between human and multiple species. |
| Predicted Species | Rat (90%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SMAD7 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Sterile - filtered PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Smad7 Antibody (NB100-56688)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SMAD7 |
![Immunohistochemistry-Paraffin: Smad7 Antibody - Azide Free [NB100-56688] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Smad7-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NB100-56688-img0003.jpg "Immunohistochemistry-Paraffin: Smad7 Antibody - Azide Free [NB100-56688] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz. Image using the standard format of this product.")
![Western Blot: Smad7 Antibody - Azide Free [NB100-56688] - Analysis using SMAD7 antibody. Lysate from A549 cells probed with SMAD7 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Smad7-Antibody---Azide-Free-Western-Blot-NB100-56688-img0002.jpg "Western Blot: Smad7 Antibody - Azide Free [NB100-56688] - Analysis using SMAD7 antibody. Lysate from A549 cells probed with SMAD7 antibody at 5 ug/ml.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")