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Smad4 Antibody [Unconjugated]

 (9 Publications)
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Smad4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2097) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Smad4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 ...read more
Jurkat human acute T cell leukemia cell line treated with 10 ng/mL Recombinant Human IL‑12 (Catalog # 219-IL) overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. ...read more
Smad4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Smad4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2097) at 15 µg/mL for 3 hours at room ...read more
Genetic Strategies: siRNA knockdown of Smad1, -2 and 4 does not impact RVFV replication.HSAECs transfected with media alone (Mock) or siRNAs (75nM) directed against a nontargeting control (NTC), Smad1, -2, or -4 ...read more
Genetic Strategies: siRNA knockdown of Smad1, -2 and 4 does not impact RVFV replication.HSAECs transfected with media alone (Mock) or siRNAs (75nM) directed against a nontargeting control (NTC), Smad1, -2, or -4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2097
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2097-SP
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Smad4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Smad4
Pro139-Asp332
Accession # Q13485
Specificity
Detects human Smad4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human (rh) Smad1 and less than 5% cross-reactivity with rhSmad9, rhSmad5, and rhSmad7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SMAD4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF2097 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad4 Antibody [Unconjugated]

  • DPC4
  • DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
  • hSMAD4
  • JIP
  • MAD homolog 4
  • MADH4
  • MADH4mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic, Drosophila, homolog of, 4
  • SMAD 4
  • SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
  • SMAD, mothers against DPP homolog 4
  • Smad4

Background

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Upon signal-induced phosphorylation, Smad subunits associate with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad4 Antibody (AF2097)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ChIP, ICC, Immunoprecipitation, Simple Western, Western Blot.


Filter By Application
ChIP
(1)
ICC
(1)
Immunoprecipitation
(2)
Simple Western
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF2097 Applications Species
LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer Cell Rep, 2020-10-13;33(2):108253. 2020-10-13 [PMID: 33053339] (Immunoprecipitation, ICC, Simple Western, Human) Immunoprecipitation, ICC, Simple Western Human
Yuan Fang, Xu Han, Jianang Li, Tiantao Kuang, Wenhui Lou HEATR1 Deficiency Promotes Chemoresistance via Upregulating ZNF185 and Downregulating SMAD4 in Pancreatic Cancer Journal of Oncology 2020-05-26 [PMID: 32565799]
Cynthia de la Fuente, Chelsea Pinkham, Deemah Dabbagh, Brett Beitzel, Aura Garrison, Gustavo Palacios et al. Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection PLOS ONE 2018-02-06 [PMID: 29408900]
N Kumeda, Y Ogawa, Y Akimoto, H Kawakami, M Tsujimoto, R Yanoshita Characterization of Membrane Integrity and Morphological Stability of Human Salivary Exosomes Biol. Pharm. Bull., 2017-01-01;40(8):1183-1191. 2017-01-01 [PMID: 28768999] (Western Blot, Human) Western Blot Human
Jie Yang, Ya Wang, Zhen Zeng, Long Qiao, Liang Zhuang, Qinglei Gao et al. Smad4 deletion in blood vessel endothelial cells promotes ovarian cancer metastasis International Journal of Oncology 2017-05-01 [PMID: 28393199]
MJ Moen, HH Adams, JH Brandsma, DH Dekkers, U Akinci, S Karkampoun, M Quevedo, CE Kockx, Z Ozgür, WF van IJcken, J Demmers, RA Poot An interaction network of mental disorder proteins in neural stem cells Transl Psychiatry, 2017-04-04;7(4):e1082. 2017-04-04 [PMID: 28375211] (Immunoprecipitation, Human) Immunoprecipitation Human
LM de Kroon, R Narcisi, GG van den Ak, EL Vitters, EN Blaney Dav, GJ van Osch, PM van der Kr SMAD3 and SMAD4 have a more dominant role than SMAD2 in TGF?-induced chondrogenic differentiation of bone marrow-derived mesenchymal stem cells Sci Rep, 2017-02-27;7(0):43164. 2017-02-27 [PMID: 28240243] (Western Blot, Human) Western Blot Human
Kazunobu Isogaya, Daizo Koinuma, Shuichi Tsutsumi, Roy-Akira Saito, Keiji Miyazawa, Hiroyuki Aburatani et al. A Smad3 and TTF-1/NKX2-1 complex regulates Smad4-independent gene expression Cell Research 2014-08-01 [PMID: 25060702]
Kim SW, Yoon SJ, Chuong E, Oyolu C, Wills AE, Gupta R, Baker J Chromatin and transcriptional signatures for Nodal signaling during endoderm formation in hESCs. Dev. Biol., 2011-06-29;357(2):492-504. 2011-06-29 [PMID: 21741376] (ChIP, Human) ChIP Human

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Bioinformatics

Gene Symbol SMAD4
Uniprot