I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?

Name: Smad3 Antibody
Brand: Novus Biologicals
SKU: NB100-56479
Rating: 3.2 (5 reviews)

Question

Accepted Answer

We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Po, Bv, Ca, Pm, Ch, Xp, ZeSpecies Glossary
Applications	WB, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	1.0 mg/ml

Immunogen	A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Predicted Species	Zebrafish (90%), Chicken (90%), Xenopus (90%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	SMAD3
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein G purified

Publications using NB100-56479	Applications	Species
Salemi S, Schori L, Gerwinn T et al. Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction International Journal of Molecular Sciences 2023-02-24 [PMID: 36901894]
Chen B, Li R, Hernandez S et al. Differential effects of Smad2 and Smad3 in regulation of macrophage phenotype and function in the infarcted myocardium Journal of Molecular and Cellular Cardiology 2022-07-01 [PMID: 35780861]
Smith SS, Chu D, Qu T et al. Species-specific sensitivity to TGF? signaling and changes to the Mmp13 promoter underlie avian jaw development and evolution eLife 2022-06-06 [PMID: 35666955] (WB)	WB
Wu X, Wang H, Chen H et al. Overexpression of smad7 inhibits the TGF-beta/Smad signaling pathway and EMT in NPHP1-defective MDCK cells Biochem Biophys Res Commun 2021-12-10 [PMID: 34689106] (WB, ICC/IF, Canine)	WB, ICC/IF	Canine
Li Y, Zhao S, Xu S et al. Palmitoylethanolamide (PEA) reduces postoperative adhesions after experimental strabismus surgery in rabbits by suppressing canonical and non-canonical TGF beta signaling through PPAR alpha Biochemical pharmacology 2020-12-29 [PMID: 33385371] (WB, Mouse)	WB	Mouse
Chen SM, Hsu LJ, Lee HL et al. Lactobacillus Attenuate the Progression of Pancreatic Cancer Promoted by Porphyromonas Gingivalis in K-rasG12D Transgenic Mice Cancers (Basel) 2020-11-26 [PMID: 33255941] (IHC-P, Mouse) Details: Pancreas samples from transgenic mice exposed to P. gingivalis underwent immunohistochemical analysis.	IHC-P	Mouse
Sivadas VP, George NA, Kattoor J, Kannan S. Novel mutations and expression alterations in SMAD3/TGFBR2 genes in oral carcinoma correlate with poor prognosis. Genes Chromosomes Cancer 2013-11-01 [PMID: 23913824] (WB, Human)	WB	Human
Ruebel KH, Leontovich AA, Tanizaki Y et al. Effects of TGFbeta1 on gene expression in the HP75 human pituitary tumor cell line identified by gene expression profiling. Endocrine. 2008-02-01 [PMID: 18401765] (WB, Human)	WB	Human

Smad3 Antibody

Species Glossary

Images

Product Details

Order Details

Smad3 Antibody Summary

Applications/Dilutions

Packaging, Storage & Formulations

Alternate Names for Smad3 Antibody

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Smad3 Antibody (NB100-56479)(8)

Reviews for Smad3 Antibody (NB100-56479) (5) 3.25

Summary

Summary

Comments

Summary

Comments

Summary

Comments

Summary

Comments

Product General Protocols

Video Protocols

FAQs for Smad3 Antibody (NB100-56479). (Showing 1 - 1 of 1 FAQs).

Control Lysate(s)

Secondary Antibodies

Isotype Controls

Additional Smad3 Products

Research Areas for Smad3 Antibody (NB100-56479)

Blogs on Smad3.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Recent Reviews

Bioinformatics

Gene Symbol	SMAD3
Entrez	17127(Mouse) 25631(Rat)
Uniprot	NP_005893(Human)

	Verified Customer 08/02/2021
Application:	WB
Species:	Mouse

	Verified Customer 05/26/2021
Application:	WB
Species:	mice

	Verified Customer 05/26/2021
Application:	WB
Species:	Bovine