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Human Smad3 ELISA Kit (Colorimetric)

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ELISA: Human Smad3 ELISA Kit (Colorimetric) [NBP2-76622] - Samples were spiked with high concentrations of Human Smad3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Smad3 ELISA Kit (Colorimetric) [NBP2-76622] - Samples were spiked with high concentrations of Human Smad3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human Smad3 ELISA Kit (Colorimetric) [NBP2-76622] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.625 - 40ng/mL
Sensitivity
0.375ng/mL

Order Details

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Human Smad3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Smad3/MADH3 in samples. No significant cross-reactivity or interference between Human Smad3/MADH3 and analogues was observed.
Standard Curve Range
0.625 - 40ng/mL
Sensitivity
0.375ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.1
Intra-Assay
CV% < 5.62
Spike Recovery
86-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SMAD3

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-76622.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Smad3 ELISA Kit (Colorimetric)

  • DKFZp586N0721
  • DKFZp686J10186
  • hMAD-3
  • hSMAD3
  • HsT17436
  • JV15-2MGC60396
  • MAD homolog 3
  • mad protein homolog
  • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
  • MAD, mothers against decapentaplegic homolog 3
  • mad3
  • MADH3mad homolog JV15-2
  • mothers against decapentaplegic homolog 3
  • Mothers against DPP homolog 3
  • SMA- and MAD-related protein 3
  • SMAD 3
  • SMAD family member 3HSPC193
  • SMAD, mothers against DPP homolog 3 (Drosophila)
  • SMAD, mothers against DPP homolog 3
  • Smad3

Background

The protein encoded by the Smad3 gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human Smad3 ELISA
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NBP2-76622
Human Smad3 ELISA Kit (Colorimetric)
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Publications for Smad3 ELISA Kit (NBP2-76622)(1)


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Product General Protocols

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FAQs for Smad3 ELISA Kit (NBP2-76622). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
    • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

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Product PDFs

  SDS
  Human Smad3 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol SMAD3