Smad3 Antibodies
We offer Smad3 Antibodies for use in common research applications: Chromatin Immunoprecipitation, ELISA, Flow (Intracellular), Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each Smad3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Smad3 Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Human, Mouse, Porcine, Primate, Rat, Xenopus, Zebrafish. Use the list below to choose the Smad3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Smad3 polyclonal antibodies and browse our Smad3 monoclonal antibody catalog.
Alternate Names for Smad3 Antibodies
anti-Smad3 antibody, anti-DKFZp586N0721 antibody, anti-DKFZp686J10186 antibody, anti-hMAD-3 antibody, anti-hSMAD3 antibody, anti-HsT17436 antibody, anti-JV15-2MGC60396 antibody, anti-MAD homolog 3 antibody, anti-mad protein homolog antibody, anti-MAD, mothers against decapentaplegic homolog 3 (Drosophila) antibody, anti-MAD, mothers against decapentaplegic homolog 3 antibody, anti-mad3 antibody, anti-MADH3mad homolog JV15-2 antibody, anti-mothers against decapentaplegic homolog 3 antibody, anti-Mothers against DPP homolog 3 antibody, anti-SMA- and MAD-related protein 3 antibody, anti-SMAD 3 antibody, anti-SMAD family member 3HSPC193 antibody, anti-SMAD, mothers against DPP homolog 3 (Drosophila) antibody, anti-SMAD, mothers against DPP homolog 3 antibody
15 Results for "smad3" in Primary Antibodies
![Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Smad3-Antibody-Western-Blot-NB100-56479-img0008.jpg "Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Smad3 at 10 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Smad3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56479-img0006.jpg)
![Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/mL.](http://images.novusbio.com/fullsize/Smad3-Antibody-Immunohistochemistry-Paraffin-NB100-56479-img0004.jpg)
![Western Blot: Smad3 Antibody [NB100-56479] - Analysis using SMAD3 antibody. Lysate from human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver and 4) rat liver probed with SMAD3 antibody at 0.5 ug/mL. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Smad3-Antibody-Western-Blot-NB100-56479-img0005.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. YIgG Zhang, NIH, CCR, Bethesda, MD.](http://images.novusbio.com/fullsize/Smad3-[p-Ser423--p-Ser425]-Antibody-Western-Blot-NBP1-77836-img0005.jpg "Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. YIgG Zhang, NIH, CCR, Bethesda, MD.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - BH4 precursor, sepiapterin, normalizes NO level and ameliorates malignant phenotype of precancerous and cancerous cells. Representative images of the progression series cultivated in 3D ECM with or without sepiapterin (20 uM) and stained for phospho-SMAD3 or ERBB2. Nuclei were counterstained with DAPI (blue). Scale bars: 20 um. (Bottom) Quantification of phospho-SMAD3 (left) and ERBB2 levels (right) of the progression series. Error bars: mean +/- STDEV. *p < 0.05; **p < 0.01 and ***p < 0.001. Note that the effects of sepiapterin are only discernible in precancerous and cancerous cells, but not in non-malignant cells. (See the results of different concentrations of sepiapterin in Supplementary Fig. 8). Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-43239-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Smad3-[p-Ser423--p-Ser425]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77836-img0007.jpg)
![Immunohistochemistry: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/Smad3-[p-Ser423--p-Ser425]-Antibody-Immunohistochemistry-NBP1-77836-img0006.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M02), clone 7F3.Lane 1: SMAD3 transfected lysate(48 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0024.jpg "Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M02), clone 7F3.Lane 1: SMAD3 transfected lysate(48 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Immunohistochemistry-Paraffin-H00004088-M02-img0017.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in HeLa.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0019.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in PC-12.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0020.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in 293.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0021.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in Jurkat.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0022.jpg)
![Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in human colon.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Western-Blot-H00004088-M02-img0023.jpg)
![Proximity Ligation Assay: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of protein-protein interactions between MAPK8 and SMAD3. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-SMAD3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-Proximity-Ligation-Assay-H00004088-M02-img0015.jpg)
![ELISA: Smad3 Antibody (7F3) [H00004088-M02] - Detection limit for recombinant GST tagged SMAD3 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smad3-Antibody-7F3-ELISA-H00004088-M02-img0025.jpg)
![Western Blot: Smad3 Antibody [NBP2-20411] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad3-Antibody-Western-Blot-NBP2-20411-img0012.jpg "Western Blot: Smad3 Antibody [NBP2-20411] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SMAD3 Antibody [NBP2-20411] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SMAD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20411-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad3 Antibody [NBP2-20411] - Human gastric cancer. SMAD2 / SMAD3 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Smad3-Antibody-Immunohistochemistry-Paraffin-NBP2-20411-img0005.jpg)
![Chromatin Immunoprecipitation: SMAD3 Antibody [NBP2-20411] - Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of SMAD3 antibody The precipitated DNA was detected by PCR with primer set targeting to smad7 promoter.](http://images.novusbio.com/fullsize/SMAD3-Antibody-Chromatin-Immunoprecipitation-NBP2-20411-img0004.jpg)
![Western Blot: Smad3 Antibody [NBP2-20411] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad3-Antibody-Western-Blot-NBP2-20411-img0007.jpg)
![Western Blot: Smad3 Antibody [NBP2-20411] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 / SMAD3 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad3-Antibody-Western-Blot-NBP2-20411-img0010.jpg)
![Immunoprecipitation: Smad3 Antibody [NBP2-20411] - HeLa whole cell extracts using 2 ug of SMAD2 / SMAD3 antibody. Western blot analysis was performed using SMAD2 / SMAD3 antibody. EasyBlot HRP-conjugated anti rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad3-Antibody-Immunoprecipitation-NBP2-20411-img0008.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on A549 cell lysates. Lane 1 : A549 cells, whole cell lysate, 10 ug /lane. Lane 2 : A549 cells were treated with 5 ng/ml TGF beta1 for 24 hours, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-Smad3 [p Ser423, p Ser425] (1/500) , Anti-Smad3 antibody (1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 3 seconds](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0006.jpg "Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on A549 cell lysates. Lane 1 : A549 cells, whole cell lysate, 10 ug /lane. Lane 2 : A549 cells were treated with 5 ng/ml TGF beta1 for 24 hours, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-Smad3 [p Ser423, p Ser425] (1/500) , Anti-Smad3 antibody (1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 3 seconds")
![Immunocytochemistry/Immunofluorescence: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Staining Phospho-Smad3(S423/S425) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunocytochemistry-Immunofluorescence-NBP2-67513-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Smad3 [p Ser423, p Ser425) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0009.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Phospho-Smad3(S423/S425) on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0003.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Lane 1: K562 cells, whole cell lysate, 10ug/lane, Lane 2: K562 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-Phospho-Smad3(S423/S425) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 48 kDa Observed band size: 55 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0004.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on different lysates with Rabbit anti-Smad3 [p Ser423, p Ser425] antibody at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse skin tissue using anti-Phospho-Smad3(S423/S425) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded human large intestine tissue with Rabbit anti-Phospho-Smad3 [p Ser423, p Ser425] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0007.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Smad3 [p Ser423, p Ser425] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0008.jpg)
![Western Blot: Smad3 Antibody (7L6K7) [NBP3-15672] - Western blot analysis of extracts of A-431 cells, using Smad3 antibody (NBP3-15672) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Smad3-Antibody-7L6K7-Western-Blot-NBP3-15672-img0004.jpg "Western Blot: Smad3 Antibody (7L6K7) [NBP3-15672] - Western blot analysis of extracts of A-431 cells, using Smad3 antibody (NBP3-15672) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Smad3 Antibody (7L6K7) [NBP3-15672] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad3 Rabbit mAb (NBP3-15672) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Smad3-Antibody-7L6K7-Immunocytochemistry-Immunofluorescence-NBP3-15672-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad3 Antibody (7L6K7) [NBP3-15672] - Immunohistochemistry of paraffin-embedded human liver using Smad3 Rabbit mAb (NBP3-15672) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Smad3-Antibody-7L6K7-Immunohistochemistry-Paraffin-NBP3-15672-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Smad3 Antibody (7L6K7) [NBP3-15672] - Western blot analysis of extracts from normal (control) and Smad3 knockout (KO) 293T cells, using Smad3 antibody (NBP3-15672) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Smad3-Antibody-7L6K7-Knockout-Validated-NBP3-15672-img0001.jpg)
![Immunoprecipitation: Smad3 Antibody (7L6K7) [NBP3-15672] - Immunoprecipitation analysis of 300ug extracts of A-431 cells using 3ug Smad3 antibody (NBP3-15672). Western blot was performed from the immunoprecipitate using Smad3 antibody (NBP3-15672) at a dilition of 1:500.](http://images.novusbio.com/fullsize/Smad3-Antibody-7L6K7-Immunoprecipitation-NBP3-15672-img0005.jpg)
![Western Blot: Smad3 [p Thr179] Antibody [NBP2-19126] - NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.](http://images.novusbio.com/fullsize/Smad3-[p-Thr179]-Antibody-Western-Blot-NBP2-19126-img0001.jpg "Western Blot: Smad3 [p Thr179] Antibody [NBP2-19126] - NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.")
![ELISA: Smad3 [p Thr179] Antibody [NBP2-19126] - The SMAD pathway follows the canonical TGF- Beta signaling pathway. TGF- Beta dimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and phosphorylates a receptor regulated SMAD.i.e. SMAD2 or SMAD3. The R-SMAD then binds to the common SMAD i.e. SMAD4, and forms a heterodimeric complex. This complex then enters the cell nucleus and acts as a transcription factor.](http://images.novusbio.com/fullsize/Smad3 [p Thr179] Antibody-ELISA-NBP2-19126-img0003.jpg)
![Western Blot: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Western blot analysis using NBP3-21516 in A549 cell lysate treated with TGF-beta 1.](http://images.novusbio.com/fullsize/nbp3-21516_rabbit-smad3-p-ser-423-mab-sr1995-6720239533715.jpg "Western Blot: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Western blot analysis using NBP3-21516 in A549 cell lysate treated with TGF-beta 1.")
![Immunohistochemistry: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using NBP3-21516.](http://images.novusbio.com/fullsize/nbp3-21516_rabbit-smad3-p-ser-423-mab-sr1995-67202310621.jpg)
![Immunocytochemistry/Immunofluorescence: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Immunofluorescent analysis of A549 cells treated with TGF-beta 1 , using NBP3-21516.](http://images.novusbio.com/fullsize/nbp3-21516_rabbit-smad3-p-ser-423-mab-sr1995-6720231051261.jpg)
![Immunocytochemistry/Immunofluorescence: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255] - Immunofluorescence Analysis of PFA-fixed HeLa cells using Smad3 Mouse Monoclonal Antibody (PCRP-SMAD3-1A2) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/Smad3-Antibody-PCRP-SMAD3-1A2-Immunocytochemistry-Immunofluorescence-NBP3-07255-img0003.jpg "Immunocytochemistry/Immunofluorescence: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255] - Immunofluorescence Analysis of PFA-fixed HeLa cells using Smad3 Mouse Monoclonal Antibody (PCRP-SMAD3-1A2) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).")
![Flow Cytometry: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255] - Flow Cytometric Analysis of PFA-fixed HeLa cells. Smad3 Mouse Monoclonal Antibody (PCRP-Smad3-1A2) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/Smad3-Antibody-PCRP-SMAD3-1A2-Flow-Cytometry-NBP3-07255-img0002.jpg)
![Protein Array: Smad3 Antibody (PCRP-SMAD3-1A2) [NBP3-07255] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Smad3 Mouse Monoclonal Antibody (PCRP-Smad3-1A2).](http://images.novusbio.com/fullsize/Smad3-Antibody-PCRP-SMAD3-1A2-Protein-Array-NBP3-07255-img0001.jpg)
![Western Blot: Smad3 Antibody [NBP2-57918] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/Smad3-Antibody-Western-Blot-NBP2-57918-img0002.jpg "Western Blot: Smad3 Antibody [NBP2-57918] - Analysis in human cell line RT-4.")
![Immunohistochemistry-Paraffin: Smad3 Antibody [NBP2-57918] - Staining of human prostate shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Smad3-Antibody-Immunohistochemistry-Paraffin-NBP2-57918-img0001.jpg)
![Immunoprecipitation: Smad3 Antibody [NBP3-14724] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from PANC-1 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiSMAD3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. SMAD3was also immunoprecipitated by rabbit anti-SMAD3antibody. For blotting immunoprecipitatedSMAD3, was used at 1:1000.Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Smad3-Antibody-Immunoprecipitation-NBP3-14724-img0001.jpg "Immunoprecipitation: Smad3 Antibody [NBP3-14724] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from PANC-1 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiSMAD3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. SMAD3was also immunoprecipitated by rabbit anti-SMAD3antibody. For blotting immunoprecipitatedSMAD3, was used at 1:1000.Chemiluminescence with an exposure time of 10 seconds.")