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GARS Antibody

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Western Blot: GARS Antibody [NBP1-85533] - Analysis using Anti-GARS antibody NBP1-85533 (A) shows similar pattern to independent antibody NBP1-85534 (B).
Independent Antibodies: Western Blot: GARS Antibody [NBP1-85533] - Analysis using Anti-GARS antibody NBP1-85533 (A) shows similar pattern to independent antibody NBP1-85534 (B).
Immunocytochemistry/ Immunofluorescence: GARS Antibody [NBP1-85533] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GARS Antibody [NBP1-85533] - Staining of human liver.
Immunohistochemistry-Paraffin: GARS Antibody [NBP1-85533] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Independent Antibodies: Immunohistochemistry-Paraffin: GARS Antibody [NBP1-85533] - Staining of human cerebral cortex, liver, pancreas and testis using Anti-GARS antibody NBP1-85533 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: GARS Antibody [NBP1-85533] - Staining of human testis.
Immunohistochemistry-Paraffin: GARS Antibody [NBP1-85533] - Staining of human pancreas.
Immunohistochemistry-Paraffin: GARS Antibody [NBP1-85533] - Staining of human cerebral cortex.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

        

Order Details

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GARS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQI
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GARS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GARS Protein (NBP1-85533PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GARS Antibody

  • AP-4-A synthetase
  • Charcot-Marie-Tooth neuropathy 2D
  • Charcot-Marie-Tooth neuropathy, neuronal type, D
  • Diadenosine tetraphosphate synthetase
  • DSMAV
  • glycine tRNA ligase
  • Glycine--tRNA ligase
  • glycyl-tRNA synthetase
  • GlyRSEC 6.1.1.14
  • HMN5
  • SMAD1CMT2D

Background

GARS encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GARS1