![Western Blot: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Western blot analysis using NBP3-21516 in A549 cell lysate treated with TGF-beta 1.](http://images.novusbio.com/fullsize/nbp3-21516_rabbit-smad3-p-ser-423-mab-sr1995-6720239533715.jpg "Western Blot: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Western blot analysis using NBP3-21516 in A549 cell lysate treated with TGF-beta 1.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | SR1995 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthesized peptide derived from human phospho-Smad3 [Ser423, Ser425] (Uniprot #: P84022) |
| Modification | p Ser423 |
| Specificity | Detects endogenous levels of total phospho-Smad3 [Ser423, Ser425] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SMAD3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl, 50% glycerol. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Simple Western EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg)
![Western Blot EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg)
![Immunocytochemistry EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg)
![Smad3 [p Ser423] Antibody (SR1995)](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Smad3 [p Ser423] Antibody (SR1995)")
Secondary Antibodies |
Isotype Controls |
Research Areas for Smad3 Antibody (NBP3-21516)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SMAD3 |
![Immunohistochemistry: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using NBP3-21516.](http://images.novusbio.com/fullsize/nbp3-21516_rabbit-smad3-p-ser-423-mab-sr1995-67202310621.jpg "Immunohistochemistry: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma, using NBP3-21516.")
![Immunocytochemistry/Immunofluorescence: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Immunofluorescent analysis of A549 cells treated with TGF-beta 1 , using NBP3-21516.](http://images.novusbio.com/fullsize/nbp3-21516_rabbit-smad3-p-ser-423-mab-sr1995-6720231051261.jpg "Immunocytochemistry/Immunofluorescence: Smad3 [p Ser 423] Antibody (SR1995) [NBP3-21516] - Immunofluorescent analysis of A549 cells treated with TGF-beta 1 , using NBP3-21516.")
![Western Blot Smad2/3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad3_AF3797_Western_Blot_5347.jpg)
![Chromatin Immunoprecipitation (ChIP) Smad2/3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad3_AF3797_Chromatin_Immunoprecipitation_11240.jpg)
![Immunocytochemistry Smad2/3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad3_AF3797_Immunocytochemistry_11829.jpg)
![Chromatin Immunoprecipitation (ChIP) Smad4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad4_AF2097_Chromatin_Immunoprecipitation_11225.jpg)
![Western Blot Smad4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad4_AF2097_Western_Blot_17168.jpg)
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![Bioactivity TGF-beta 1 [Unconjugated]](https://images.novusbio.com/images/protein/7754-bhcf_recombinant-human-tgf-beta-1-human-cell-expressed-cf-bioactivity-1811202013946.jpg)
![Western Blot Smad7 Antibody (293039) [Unconjugated]](https://images.novusbio.com/images2/Smad7_MAB2029_Western_Blot_6004.jpg)
![Immunohistochemistry Smad7 Antibody (293039) [Unconjugated]](https://images.novusbio.com/images2/Smad7_MAB2029_Immunohistochemistry_6962.jpg)
![Immunohistochemistry Smad7 Antibody (293039) [Unconjugated]](https://images.novusbio.com/images2/Smad7_MAB2029_Immunohistochemistry_9846.jpg)
![Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]](https://images.novusbio.com/images2/TGFbeta_RI_AF3025_Western_Blot_17081.jpg)
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![Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]](https://images.novusbio.com/images/af3025_human-tgf-beta-ri-alk-5-affinity-purified-polyclonal-ab-41202412402510.jpg)
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![Western Blot CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-822024951141.jpg)
![Immunohistochemistry Smad1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad1_AF2039_Immunohistochemistry_10106.jpg)
![Western Blot Smad1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad1_AF2039_Western_Blot_5162.jpg)
![Simple Western Smad1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad1_AF2039_Simple_Western_16677.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")