RPA70 Antibody

Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA1, the 70 kDa subunit, is an ssDNA binding protein that has been shown to be phosphorylated by ATM and ATR in response to DNA damage. In addition to a role in MMR, RPA also functions in the initiation of DNA replication and DNA unwinding at origins of replication. Alternate names for RPA70 include replication protein A 70 kDa DNA-binding subunit, RP-A, RF-A, Replication factor-A protein 1, single-stranded DNA-binding protein, p70, REPA1, RPA1, and HSSB.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.

Showing Publications 1 - 7 of 7.

Publications using NB100-2204	Applications	Species
Chatzidoukaki O, Stratigi K, Goulielmaki E Et al. R-loops trigger the release of cytoplasmic ssDNAs leading to chronic inflammation upon DNA damage Sci Adv 2021-11-19 [PMID: 34797720] (WB, Mouse) Details: Citation using the DyLight 550 version of this antibody.	WB	Mouse
Lindsey-Boltz LA, Reardon JT, Wold MS et al. In vitro analysis of the role of replication protein A (RPA) and RPA phosphorylation in ATR-mediated checkpoint signaling. J Biol Chem 2012-10-01 [PMID: 22948311]
Kemp MG, Reardon JT, Lindsey-Boltz LA et al. Mechanism of release and fate of excised oligonucleotides during nucleotide excision repair. J Biol Chem 2012-06-01 [PMID: 22573372]
Fisher LA, Bessho M, Wakasugi M et al. Role of interaction of XPF with RPA in nucleotide excision repair. J Mol Biol 2011-10-01 [PMID: 21875596]
Kemp MG, Lindsey-Boltz LA, Sancar A et al. The DNA damage response kinases DNA-dependent protein kinase (DNA-PK) and ataxia telangiectasia mutated (ATM) Are stimulated by bulky adduct-containing DNA. J Biol Chem 2011-06-01 [PMID: 21487018]
Thangavel S, Mendoza-Maldonado R, Tissino E et al. Human RECQ1 and RECQ4 helicases play distinct roles in DNA replication initiation. Mol Cell Biol 2010-03-01 [PMID: 20065033]
Robison JG, Elliott J, Dixon K et al. Replication protein A and the Mre11.Rad50.Nbs1 complex co-localize and interact at sites of stalled replication forks. J Biol Chem 2004-08-01 [PMID: 15180989]

Array NB100-2204

• RPA70 Antibodies
• RPA70 ELISA Kits
• RPA70 Lysate
• RPA70 Proteins