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POLR1B Antibody (4H6)

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Western Blot: POLR1B Antibody (4H6) [H00084172-M10] - Analysis of POLR1B expression in transfected 293T cell line by POLR1B monoclonal antibody (M10), clone 4H6. Lane 1: POLR1B transfected lysate (Predicted MW: 128.2 KDa). Lane 2: Non-transfected lysate.
Western Blot: POLR1B Antibody (4H6) [H00084172-M10] - Analysis of POLR1B expression in transfected 293T cell line by POLR1B monoclonal antibody (M10), clone 4H6. Lane 1: POLR1B transfected lysate (Predicted MW: 128.2 ...read more
Immunocytochemistry/ Immunofluorescence: POLR1B Antibody (4H6) [H00084172-M10] - Analysis of monoclonal antibody to POLR1B on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: POLR1B Antibody (4H6) [H00084172-M10] - Detection limit for recombinant GST tagged POLR1B is 3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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POLR1B Antibody (4H6) Summary

Immunogen
POLR1B (NP_061887, 963 a.a. ~ 1072 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GEMLKAAGYNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVK
Specificity
POLR1B (4H6)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POLR1B
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POLR1B Antibody (4H6)

  • DNA-directed RNA polymerase I 135 kDa polypeptide
  • DNA-directed RNA polymerase I 135kDa polypeptide
  • DNA-directed RNA polymerase I subunit RPA2
  • EC 2.7.7.6
  • FLJ10816
  • FLJ21921
  • MGC131780
  • polymerase (RNA) I polypeptide B, 128kDa
  • RNA polymerase I subunit 2
  • RPA116
  • RPA135
  • RPA2
  • Rpo1-2

Background

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Array H00084172-M10

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Bioinformatics

Gene Symbol POLR1B
Entrez
Uniprot