Chk1 Antibody

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NB100-97831

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IF/IHC, IP, WB.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NB100-464	Applications	Species
Lee KY, Dutta A Chk1 promotes non-homologous end joining in G1 through direct phosphorylation of ASF1A Cell reports 2021-01-26 [PMID: 33503415] (IP, Human)	IP	Human
Morris BB, Wages NA, Grant PA, et al. MYBL2-Driven Transcriptional Programs Link Replication Stress and Error-prone DNA Repair With Genomic Instability in Lung Adenocarcinoma Frontiers in oncology 2020-01-08 [PMID: 33489883] (WB, Human)	WB	Human
Shibata, E & Dutta, A. A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteins. J Biol Chem 2020-12-11 [PMID: 33453950] (IF/IHC, Human)	IF/IHC	Human
Shibata E, Dutta A A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteins The Journal of biological chemistry 2020-12-11 [PMID: 32989049] (WB, Human)	WB	Human
Bai X, Wang J, Huo L et al. Serine/Threonine Kinase CHEK1-Dependent Transcriptional Regulation of RAD54L Promotes Proliferation and Radio Resistance in Glioblastoma. Transl Oncol 2017-12-26 [PMID: 29287241] (Mouse)		Mouse
Shibata E, Kiran M, Shibata Y et al. Two subunits of human ORC are dispensable for DNA replication and proliferation Elife. 2016-11-30 [PMID: 27906128] (WB, Human)	WB	Human
Yun H, Shi R, Yang Q et al. Over expression of hRad9 protein correlates with reduced chemosensitivity in breast cancer with administration of neoadjuvant chemotherapy Sci Rep. 2014-12-17 [PMID: 25520248] (IF/IHC, Human)	IF/IHC	Human
Terai K, Shibata E, Abbas T, Dutta A. Degradation of p12 Subunit by CRL4Cdt2 E3 Ligase Inhibits Fork Progression after DNA Damage. J Biol Chem. 2013-10-18 [PMID: 24022480] (WB, Human)	WB	Human
Jazaeri AA, Shibata E, Park J et al. Overcoming platinum resistance in preclinical models of ovarian cancer using the neddylation inhibitor MLN4924. Mol Cancer Ther. 2013-08-12 [PMID: 23939375] (WB, Human)	WB	Human
Hong J, Hu K, Yuan Y et al. CHK1 targets spleen tyrosine kinase (L) for proteolysis in hepatocellular carcinoma J Clin Invest 2012-06-01 [PMID: 22585575] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Karnani N, Dutta A. The effect of the intra-S-phase checkpoint on origins of replication in human cells. Genes Dev;25(6):621-33. 2011-03-15 [PMID: 21406556]
Shiotani B, Kobayashi M, Watanabe M et al. Involvement of the ATR- and ATM-dependent checkpoint responses in cell cycle arrest evoked by pierisin-1. Mol Cancer Res 2006-02-01 [PMID: 16513843]
Show All 12 Publications. Collapse Publications.

Array NB100-464

• Chk1 Antibodies
• Chk1 Lysates
• Chk1 Protein
• Chk1 Proteins and Enzyme