RPA2 [p Ser4, p Ser8] Antibody

Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ICC/IF, Immunohistochemistry, Immunoprecipitation, KD, WB, Western Blot.

Showing Publications 1 - 10 of 42. Show All 42 Publications. Collapse Publications.

Publications using NBP1-23017	Applications	Species
Zhang S, Zhao Y, Wang X et al. Synergistic lethality between auranofin-induced oxidative DNA damage and ATR inhibition in cancer cells Life sciences 2023-11-01 [PMID: 37778414] (Western Blot, Human)	Western Blot	Human
Liao C, Talluri S, Zhao J et al. RAD51 Is Implicated in DNA Damage, Chemoresistance and Immune Dysregulation in Solid Tumors Cancers 2022-11-20 [PMID: 36428789] (WB, Human)	WB	Human
de Krijger I, FOhr B, PErez SH et al. MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair Nature communications 2021-09-14 [PMID: 34521823] (WB, Human)	WB	Human
Niu J, Wang J, Zhang Q et al. Cinobufagin-induced DNA damage response activates G(2)/M checkpoint and apoptosis to cause selective cytotoxicity in cancer cells Cancer Cell International 2021-12-01 [PMID: 34425836] (Immunoprecipitation, Western Blot)	Immunoprecipitation, Western Blot
Kumar S, Buon L, Talluri S et al. Integrated genomics and comprehensive validation reveal drivers of genomic evolution in esophageal adenocarcinoma Communications biology 2021-05-24 [PMID: 34031527]
Venkatesan S, Angelova M, Puttick C et al. Induction of APOBEC3 Exacerbates DNA Replication Stress and Chromosomal Instability in Early Breast and Lung Cancer Evolution Cancer Discovery 2021-10-01 [PMID: 33947663] (Immunohistochemistry)	Immunohistochemistry
Shinoda K, Zong D, Callen E et al. The dystonia gene THAP1 controls DNA double-strand break repair choice Molecular cell 2021-04-06 [PMID: 33857404]
Li H, Ma L, Bian X et al. FK228 sensitizes radioresistant small cell lung cancer cells to radiation Clinical epigenetics 2021-02-25 [PMID: 33632300] (Human)		Human
Bian X, Wang X, Zhang Q et al. The MYC Paralog-PARP1 Axis as a Potential Therapeutic Target in MYC Paralog-Activated Small Cell Lung Cancer Front. Oncol. 2020-10-08 [PMID: 33134168] (WB, Human)	WB	Human
Bartek J, Merchut-Maya JM, Maya-Mendoza A et al. Cancer cell stemness, responses to experimental genotoxic treatments, cytomegalovirus protein expression and DNA replication stress in pediatric medulloblastomas Cell Cycle 2020-02-13 [PMID: 32054408] (Human)		Human
Wang H, Zhang S et al. Synergistic lethality between PARP-trapping and alantolactone-induced oxidative DNA damage in homologous recombination-proficient cancer cells. Oncogene 2020-01-04 [PMID: 32029902] (ICC/IF, Human)	ICC/IF	Human
Fagan-Solis KD, Simpson DA, Kumar RJ et al. A P53-Independent DNA Damage Response Suppresses Oncogenic Proliferation and Genome Instability Cell Rep 2020-02-04 [PMID: 32023457] (ICC/IF, Mouse)	ICC/IF	Mouse
Warren N, Eastman A. Inhibition of checkpoint kinase 1 following gemcitabine-mediated S phase arrest results in CDC7- and CDK2-dependent replication catastrophe. J. Biol. Chem. 2018-12-20 [PMID: 30573684] (WB, Human)	WB	Human
Simonetta M, de Krijger I, Serrat J et al. H4K20me2 distinguishes pre-replicative from post-replicative chromatin to appropriately direct DNA repair pathway choice by 53BP1-RIF1-MAD2L2. Cell Cycle. [PMID: 29160738] (WB, Human)	WB	Human
zhang s, Zhao Y, Wang X et al. Sublethal Auranofin Potentiates Tumor Responses to ATR Inhibition by Inducing Oxidative DNA Damage Selectively in Cancer Cells SSRN Electronic Journal 2023-03-20
Qu J, Sun W, Zhong J et al. Correction: Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells J. Cell Biol. 2017-07-24 [PMID: 28739677] (WB, Human) Details: Novus' PGAM1 antibbody was used to look at PGAM1 levels in HeLa cells that were either treated with 100uM dNTPs or not treated	WB	Human
Qu J, Sun W, Zhong J et al. Phosphoglycerate mutase 1 regulates dNTP pool and promotes homologous recombination repair in cancer cells J Cell Biol. 2016-07-03 [PMID: 28122957] (KD, ICC/IF, WB, Human)	KD, ICC/IF, WB	Human
Sakurikar N, Thompson R, Montano R, Eastman A A subset of cancer cell lines is acutely sensitive to the Chk1 inhibitor MK-8776 as monotherapy due to CDK2 activation in S phase. Oncotarget 2016-01-12 [PMID: 26595527] (WB)	WB
Thompson R, Montano R, Eastman A et al. The Mre11 nuclease is critical for the sensitivity of cells to Chk1 inhibition. PLoS One 2012-01-01 [PMID: 22937147]
Kousholt AN, Fugger K, Hoffmann S et al. CtIP-dependent DNA resection is required for DNA damage checkpoint maintenance but not initiation. J Cell Biol 2012-06-01 [PMID: 22733999]
Punchihewa C, Inoue A, Hishiki A et al. Identification of small molecule proliferating cell nuclear antigen (PCNA) inhibitor that disrupts interactions with PIP-box proteins and inhibits DNA replication. J Biol Chem 2012-04-01 [PMID: 22383522]
Regairaz M, Zhang Y-W, Fu H et al. Mus81-mediated DNA cleavage resolves replication forks stalled by topoisomerase I-DNA complexes. J Cell Biol 28195(5):739-749. 2011-11-01 [PMID: 22123861]
Xu LH, Huang M, Fang SG, Liu DX. Coronavirus infection induces DNA replication stress partly through interaction of its nonstructural protein 13 with the p125 subunit of DNA polymerase delta. Journal of Biological Chemistry 286 (45):39546-39559. 2011-01-01 [PMID: 21918226]
Liu S, Chu J, Yucer N et al. RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response. J Biol Chem 2011-06-01 [PMID: 21558276]
Baldeyron C, Soria G, Roche D et al. HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair. J Cell Biol 2011-04-01 [PMID: 21464229]
Du G, Dutta N, Lashmit P et al. Alternative splicing of the human cytomegalovirus major immediate-early genes affects infectious-virus replication and control of cellular cyclin-dependent kinase. J Virol 2011-01-01 [PMID: 21068259]
Chen J, Luo Q, Yuan Y et al. Pygo2 associates with MLL2 histone methyltransferase and GCN5 histone acetyltransferase complexes to augment Wnt target gene expression and breast cancer stem-like cell expansion. Mol.Cell.Biol. 30 (24):5621-5635. 2010-01-01 [PMID: 20937768]
Shi W, Feng Z, Zhang J et al. The role of RPA2 phosphorylation in homologous recombination in response to replication arrest. Carcinogenesis 2010-06-01 [PMID: 20130019]
Glauser DL, Seyffert M, Strasser R et al. Inhibition of herpes simplex virus type 1 replication by adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/helicase activities. J Virol 2010-04-01 [PMID: 20106923]
Cerqueira A, Santamaria D, Martinez-Pastor B et al. Overall Cdk activity modulates the DNA damage response in mammalian cells. J Cell Biol 2009-12-01 [PMID: 19995934]
Stephan H, Concannon C, Kremmer E et al. Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis. Nucleic Acids Res 2009-10-01 [PMID: 19671522]
Huertas P, Jackson SP. Human CtIP mediates cell cycle control of DNA end resection and double strand break repair. J Biol Chem 2009-04-01 [PMID: 19202191]
Carson CT, Orazio NI, Lee DV et al. Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection. EMBO J 2009-03-01 [PMID: 19197236]
Gravel S, Chapman JR, Magill C et al. DNA helicases Sgs1 and BLM promote DNA double-strand break resection. Genes Dev 2008-10-01 [PMID: 18923075]
Kranz D, Dohmesen C, Dobbelstein M et al. BRCA1 and Tip60 determine the cellular response to ultraviolet irradiation through distinct pathways. J Cell Biol 2008-07-01 [PMID: 18625847]
Fragkos M, Breuleux M, Clement N et al. Recombinant adeno-associated viral vectors are deficient in provoking a DNA damage response. J Virol 2008-08-01 [PMID: 18463154]
Manthey KC, Opiyo S, Glanzer JG et al. NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse. J Cell Sci 2007-12-01 [PMID: 18003706]
Morency E, Sabra M, Catez F et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 2007-06-01 [PMID: 17548509]
Olson E, Nievera CJ, Klimovich V et al. RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint. J Biol Chem 2006-12-01 [PMID: 17035231]
Cruet-Hennequart S, Coyne S, Glynn MT et al. UV-induced RPA phosphorylation is increased in the absence of DNA polymerase eta and requires DNA-PK. DNA Repair (Amst) 2006-04-01 [PMID: 16520097]
Wu X, Shell SM, Zou Y et al. Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells. Oncogene 2005-07-01 [PMID: 15897895]
Vassin VM, Wold MS, Borowiec JA et al. Replication protein A (RPA) phosphorylation prevents RPA association with replication centers. Mol Cell Biol 2004-03-01 [PMID: 14966274]
Show All 42 Publications. Collapse Publications.

Array NBP1-23017

• RPA2 Antibodies
• RPA2 Lysate
• RPA2 Proteins