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Annexin A6 Antibody

Images

 
Orthogonal Strategies: Western Blot: Annexin A6 Antibody [NBP1-90149] - Analysis in human cell lines SK-MEL-30 and A-431 using anti-ANXA6 antibody. Corresponding ANXA6 RNA-seq data are presented for the same cell ...read more
Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP1-90149] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.
Western Blot: Annexin A6 Antibody [NBP1-90149] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP1-90149] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP1-90149] - Staining of human pancreas shows strong membranous positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: Annexin A6 Antibody [NBP1-90149] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Annexin A6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IADTPSGDKTSLETRFMTILCTRSYPHLRRVFQEFIKMTNYDVEHTIKKEMSGDVRDAFVAIVQSVKNKPLFFADKLYKSMKGAGTDEKTLTRIMVSRSEIDLLNIRREFIEKYDKSLHQAIEGDTSGDFLKALLALCGGED
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANXA6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Annexin A6 Protein (NBP1-90149PEP)
Publications
Read Publications using
NBP1-90149 in the following applications:

  • WB
    1 publication

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22438584)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Annexin A6 Antibody

  • 67 kDa calelectrin
  • Annexin A6
  • annexin VI (p68)
  • Annexin VI
  • Annexin-6
  • ANX6
  • ANXA6
  • calcium-binding protein p68
  • Calelectrin
  • calphobindin II
  • calphobindin-II
  • CBP68
  • chromobindin-20
  • CPB-II
  • CPB-II
  • Lipocortin VI
  • p68
  • p70
  • Protein III

Background

The Annexins constitute a family of structurally-related, relatively abundant proteins that exhibit Ca2+-dependent binding to phospholipids. Annexins function in multiple aspects of cell biology including regulation of membrane trafficking, transmembrane channel activity, inhibition of phospholipase A2, inhibition of coagulation and mediation of cell-matrix interactions. Annexin A13 is considered the original progenitor of the 12 members of vertebrate Annexins. The expression of Annexin A13 is highly tissue-specific, being expressed only in intestinal and kidney epithelial cells. This expression is associated with a highly differentiated intracellular transport function. Two alternative splicing isoforms of Annexin A13 exist, both of which bind to rafts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Annexin A6 Antibody (NBP1-90149)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ANXA6