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Recombinant Mouse TLR2 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Mouse TLR2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized peptidoglycan at 10 µg/mL (100 µL/well) can bind recombinant mouse TLR2 Fc Chimera with a linear range of 0.04-30 µg/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse TLR2 protein
Mouse TLR2
(Gln25-Leu590)
Accession # Q9QUN7
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln25 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Tlr2
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
90.4 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
92 kDa, reducing conditions
Publications
Read Publications using
1530-TR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and PEG 3350.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TLR2 Fc Chimera Protein, CF

  • CD282 antigen
  • CD282
  • TIL4CD282
  • TLR2
  • toll/interleukin 1 receptor-like 4
  • Toll/interleukin-1 receptor-like protein 4
  • toll-like receptor 2

Background

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens (1 - 4). To date, there are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1, 3). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular nucleic acids) (3). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. Mouse Toll-like receptor-2 (TLR2) is a 97 kDa, 760 amino acid (aa) glycoprotein that contains a 563 aa extracellular region, a 21 aa TM segment, and a 176 aa cytoplasmic domain (5, 6). The extracellular region contains 16 leucine-rich repeats, while the cytoplasmic tail shows one 146 aa TIR domain. The receptor is expressed on a number of cell types including T cells ( alpha beta and gamma δ), monocytes, dendritic cells, neutrophils, B cells, endothelial cells, mast cells, NK cells, macrophages and hepatocytes (1, 4, 5, 7, 8). TLR2 functions as part of a heterodimeric complex with either TLR1 or TLR6 (1, 3, 4). These complexes recognize lipoproteins and glycolipids from gram-positive and gram-negative bacteria as well as mycoplasma and yeast. TLR2/TLR1 heterodimers recognize triacylated lipopeptides from a variety of microorganisms. The TLR2/TLR6 heterodimer preferentially recognizes diacylated lipopeptides (9). Biglycan is also known to activate TLR2, but the context is unclear (8). Notably, in human, TLR2 also dimerizes with TLR10. But the TLR10 gene in mouse (but not rat) is mutationally inactive, and thus this complex is nonfunctional (10). Upon ligand recognition, TLR2 delivers an activating signal via the associated adapter molecules, MyD88 and TIRAP (1, 11). Activation via TLR2 also results in production of a number of pro-inflammatory cytokines including TNF-alpha , IL-2, IL-6, IL-12, and MIP-2 (1, 3). The extracellular region of mouse TLR2 is 89%, 67%, 81%, and 65% aa identical to the equivalent region in rat, human, hamster and canine, respectively.

 

  1. Wetzler, L. (2003) Vaccine 21:S2/55.
  2. Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
  3. Dunne, A. and L. O’Neill (2005) FEBS. Lett. 579:3330.
  4. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  5. Matsuguchi, T. et al. (2000) Blood 95:1378.
  6. Meng, G. et al. (2005) Immunol. Lett. 98:200.
  7. Flo T. et al. (2001) J. Leukoc. Biol. 69:474.
  8. Schaefer, L. et al. (2005) J. Clin. Invest. 115:2223.
  9. Akira S. (2003) Curr. Opin. Immunol. 15:5.
  10. Hasan, U. et al. (2005) J. Immunol. 174:2942.
  11. Yamamoto M. et al. (2002) Nature 420:324.

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Publications for TLR2 (1530-TR)(6)

We have publications tested in 4 confirmed species: Human, Mouse, Bacteria - Staphylococcus aureus, Staphylococcus epidermidis.

We have publications tested in 3 applications: Bioassay, ELISA (Capture), Western Blot Control.


Filter By Application
Bioassay
(4)
ELISA (Capture)
(1)
Western Blot Control
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Bacteria - Staphylococcus aureus
(1)
Staphylococcus epidermidis
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 1530-TR Applications Species
X Xie, H Lv, C Liu, X Su, Z Yu, S Song, H Bian, M Tian, C Qin, J Qi, Q Zhu HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosis Bmc Medicine, 2021-10-15;19(1):247. 2021-10-15 [PMID: 34649530] (Western Blot Control, Mouse) Western Blot Control Mouse
N Maehara, K Taniguchi, A Okuno, H Ando, A Hirota, Z Li, CT Wang, S Arai, T Miyazaki AIM/CD5L attenuates DAMPs in the injured brain and thereby ameliorates ischemic stroke Cell Reports, 2021-09-14;36(11):109693. 2021-09-14 [PMID: 34525359] (Bioassay, Human) Bioassay Human
JA George, SB Kim, JY Choi, AM Patil, FMA Hossain, E Uyangaa, J Hur, SY Park, JH Lee, K Kim, SK Eo TLR2/MyD88 pathway-dependent regulation of dendritic cells by dengue virus promotes antibody-dependent enhancement via Th2-biased immunity Oncotarget, 2017-11-20;8(62):106050-106070. 2017-11-20 [PMID: 29285314] (Bioassay, Human) Bioassay Human
Koymans K, Feitsma L, Brondijk T, Aerts P, Lukkien E, Lossl P, van Kessel K, de Haas C, van Strijp J, Huizinga E Structural basis for inhibition of TLR2 by staphylococcal superantigen-like protein 3 (SSL3). Proc Natl Acad Sci U S A, 2015-08-17;112(35):11018-23. 2015-08-17 [PMID: 26283364] (Bioassay, Bacteria - Staphylococcus aureus) Bioassay Bacteria - Staphylococcus aureus
Raby A, Le Bouder E, Colmont C, Davies J, Richards P, Coles B, George C, Jones S, Brennan P, Topley N, Labeta M Soluble TLR2 reduces inflammation without compromising bacterial clearance by disrupting TLR2 triggering. J Immunol, 2009-07-01;183(1):506-17. 2009-07-01 [PMID: 19542461] (Bioassay, Staphylococcus epidermidis) Bioassay Staphylococcus epidermidis
Onishi S, Honma K, Liang S, Stathopoulou P, Kinane D, Hajishengallis G, Sharma A Toll-like receptor 2-mediated interleukin-8 expression in gingival epithelial cells by the Tannerella forsythia leucine-rich repeat protein BspA. Infect. Immun., 2007-10-29;76(1):198-205. 2007-10-29 [PMID: 17967853] (ELISA (Capture), Mouse) ELISA (Capture) Mouse

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Additional TLR2 Products

Blogs on TLR2. Showing 1-10 of 11 blog posts - Show all blog posts.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.


  Read full blog post.

Toll-like receptor 2 activation contributes to oral squamous cell carcinoma development and miRNA-mediated drug resistance
By Jamshed Arslan, Pharm. D., PhD. Squamous cell carcinoma is the most common cancer in the oral cavity.1 The tumor surface biofilms in oral cancers contain high levels of aerobic and anaerobic microorganisms.1,2 Peri...  Read full blog post.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol Tlr2
Uniprot