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P-Selectin/CD62P Antibody (Psel.KO.2.7)

Images

 
Immunocytochemistry/ Immunofluorescence: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Alignment of live-cell calcium response behavior with PTH abundance in a parathyroid adenoma. (A) Prestimulation baseline ...read more
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Analysis using the DyLight 488 conjugate of NB100-65392. Thrombin stimulated equine platelets (solid line) and unstimulated platelets (dashed line) ...read more
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets.
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets.
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Analysis using the DyLight 488 conjugate of NB100-65392. Differential CD62P (P-selectin) expression of equine platelets in response to varying ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IP
Clone
Psel.KO.2.7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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P-Selectin/CD62P Antibody (Psel.KO.2.7) Summary

Immunogen
P-selectin transfected 300.19 cells
Localization
Membrane; single pass type I membrane protein.
Specificity
NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SELP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:25-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. IHC-Frozen use reported in literature (see Massaguer, A. et al, 2000). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 31237986).
Control
P-Selectin/CD62P Overexpression Lysate
Publications
Read Publications using
NB100-65392 in the following applications:

Reactivity Notes

Predicted cross-reactivities: Mouse, Equine, Goat, Bovine, Feline, Sheep, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for P-Selectin/CD62P Antibody (Psel.KO.2.7)

  • CD62P antigen
  • CD62P
  • FLJ45155
  • GMP140
  • GRMP
  • PADGEM
  • PADGEMantigen CD62)
  • PSEL
  • P-Selectin
  • selectin P (granule membrane protein 140kDa, antigen CD62)
  • SELP

Background

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P-Selectin/CD62P Antibody (NB100-65392)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Equine.

We have publications tested in 4 applications: FLOW, IHC, IHC-P, Immunohistochemistry.


Filter By Application
FLOW
(6)
IHC
(1)
IHC-P
(3)
Immunohistochemistry
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
Equine
(3)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB100-65392 Applications Species
Raslan, AA;Pham, TX;Lee, J;Kontodimas, K;Tilston-Lunel, A;Schmottlach, J;Hong, J;Dinc, T;Bujor, AM;Caporarello, N;Thiriot, A;von Andrian, UH;Huang, SK;Nicosia, RF;Trojanowska, M;Varelas, X;Ligresti, G; Lung injury-induced activated endothelial cell states persist in aging-associated progressive fibrosis Nature communications 2024-06-27 [PMID: 38937456]
Jheng P, Chiang C, Kang J et al. Cold atmospheric plasma-enabled platelet vesicle incorporated iron oxide nano-propellers for thrombolysis Materials Today Bio 2023-12-01 [PMID: 38089433] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Liu CH, Jheng PR, Rethi L et al. P-Selectin mediates targeting of a self-assembling phototherapeutic nanovehicle enclosing dipyridamole for managing thromboses Journal of Nanobiotechnology 2023-08-08 [PMID: 37553670] (Immunohistochemistry) Immunohistochemistry
Raslan AA, Pham TX, Lee J et al. Single Cell Transcriptomics of Fibrotic Lungs Unveils Aging-associated Alterations in Endothelial and Epithelial Cell Regeneration bioRxiv : the preprint server for biology 2023-01-20 [PMID: 36712020] (FLOW, Human)

Details:
Dilution used in IHC-P 1:200, Flow 1:20
FLOW Human
Mastrogiacomo L, Werstuck GH Investigating the Role of Endothelial Glycogen Synthase Kinase3?/? in Atherogenesis in Low Density Lipoprotein Receptor Knockout Mice International journal of molecular sciences 2022-11-26 [PMID: 36499109] (IHC, Mouse)

Details:
Dilutions: 1:50
IHC Mouse
Bondareva O, RodrIguez-Aguilera JR, Oliveira F et al. Single-cell profiling of vascular endothelial cells reveals progressive organ-specific vulnerabilities during obesity Nature metabolism 2022-11-01 [PMID: 36400935] (IHC-P, Mouse) IHC-P Mouse
Mastrogiacomo L, Ballagh R, Venegas-Pino DE et al. The Effects of Hyperglycemia on Early Endothelial Activation and the Initiation of Atherosclerosis The American journal of pathology 2022-10-12 [PMID: 36243046]
Tanaka H, Horioka K, Hasebe T et al. Treatment of hepatocellular carcinoma with autologous platelets encapsulating sorafenib or lenvatinib: A novel therapy exploiting tumor-platelet interactions International journal of cancer 2021-12-22 [PMID: 34935134]
Horioka K, Tanaka H, Isozaki S et al. Rewarming from accidental hypothermia enhances whole blood clotting properties in a murine model Thromb. Res. 2020-07-10 [PMID: 32683149] (IHC-P, Mouse) IHC-P Mouse
Horioka K, Tanaka H, Shotaro I et al. Hypothermia-induced activation of the splenic platelet pool as a risk factor for thrombotic disease in a mouse model J. Thromb. Haemost. 2019-06-25 [PMID: 31237986] (IHC-P, Mouse) IHC-P Mouse
Show All 22 Publications.

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Product General Protocols

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FAQs for P-Selectin/CD62P Antibody (NB100-65392). (Showing 1 - 3 of 3 FAQs).

  1. Does this antibody come conjugated to anything other than PE?
    • NB100-65392, clone Psel.KO.2.7 is only available conjugated to PE. We do sell kits that allow you to conjugate it to another color of your choice if you are interested. We can also do the conjugation in house for an additional fee and this can usually be done in 5-7 business days, depending on how busy the lab is. PE is our only readily available conjugation without an additional fee though.
  2. I am considering purchasing NB100-65392. Do you have a recommended isotype control to use in conjunction?
    • NBP1-43778 is an appropriate isotype control.
  3. The datasheet states that it is an IgG1 antibody. Is this IgG1 kappa or IgG1 lambda?
    • It is IgG1 kappa.
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Bioinformatics

Gene Symbol SELP
Entrez
Uniprot