P-Selectin/CD62P Antibody (Psel.KO.2.7) Summary
Immunogen |
P-selectin transfected 300.19 cells |
Localization |
Membrane; single pass type I membrane protein. |
Specificity |
NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
SELP |
Purity |
Protein A purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 1:25-1:50
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunohistochemistry-Paraffin
- Immunoprecipitation 1:10-1:500
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Application Notes |
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. IHC-Frozen use reported in literature (see Massaguer, A. et al, 2000). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 31237986). |
Control |
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Publications |
Read Publications using NB100-65392 in the following applications:
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Reactivity Notes
Predicted cross-reactivities: Mouse, Equine, Goat, Bovine, Feline, Sheep, Rat
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.09% Sodium Azide |
Concentration |
1.0 mg/ml |
Purity |
Protein A purified |
Alternate Names for P-Selectin/CD62P Antibody (Psel.KO.2.7)
Background
CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for P-Selectin/CD62P Antibody (NB100-65392). (Showing 1 - 3 of 3 FAQs).
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Does this antibody come conjugated to anything other than PE?
- NB100-65392, clone Psel.KO.2.7 is only available conjugated to PE. We do sell kits that allow you to conjugate it to another color of your choice if you are interested. We can also do the conjugation in house for an additional fee and this can usually be done in 5-7 business days, depending on how busy the lab is. PE is our only readily available conjugation without an additional fee though.
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I am considering purchasing NB100-65392. Do you have a recommended isotype control to use in conjunction?
- NBP1-43778 is an appropriate isotype control.
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The datasheet states that it is an IgG1 antibody. Is this IgG1 kappa or IgG1 lambda?
Control Lysate(s)
Secondary Antibodies
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Isotype Controls
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