PSGL-1/CD162 Antibody (HECA-452)

Cutaneous Lymphocyte Antigen (CLA) is a single-pass type I membrane homodimer protein (140kDa) that collects in normal and inflamed skin. It is expressed on T lymphocytes, subsets of peripheral blood memory T cells, Natural Killer cells, memory B cells and dendritic cells, as well as on monocytes and granulocytes. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1). CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in leukocyte tethering and rolling over vascular surfaces during inflammation. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA and is appropriate for staining formalin-fixed paraffin embedded tissue sections.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Block/Neutralize, ELISA, FLOW, IF/IHC, WB, Western Blot.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NB100-78039	Applications	Species
Qi Xu, Ming Shi, Lu Ding, Yu Xia, Liang Luo, Xiaofang Lu, Xiaoying Zhang, David Y. B. Deng High expression of P-selectin induces neutrophil extracellular traps via the PSGL-1/Syk/Ca 2+ /PAD4 pathway to exacerbate acute pancreatitis Frontiers in Immunology 2023-09-28 [PMID: 37841279]
Li GX, Jiang XH, Zang JN et al. B-cell receptor associated protein 31 deficiency decreases the expression of adhesion molecule CD11b/CD18 and PSGL-1 in neutrophils to ameliorate acute lung injury The International Journal of Biochemistry & Cell Biology 2022-11-01 [PMID: 36210579] (Western Blot, Block/Neutralize)	Western Blot, Block/Neutralize
Shi H, Jiang C, Yao H et al. CD44 fucosylation on bone marrow-derived mesenchymal stem cells enhances homing and promotes enteric nervous system remodeling in diabetic mice Cell & Bioscience 2021-12-01 [PMID: 34193268] (Mouse)		Mouse
Yeini E, Ofek P, Pozzi S et al. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression Nature communications 2021-03-26 [PMID: 33771989] (FLOW)	FLOW
Kim CH, Cheong KA, Park CD, Lee AY. Therapeutic Effects of Combination Using Glucosamine Plus Tacrolimus (FK-506) on The Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice. Scand J Immunol;75(5):471-8. 2012-05-01 [PMID: 22023698] (IF/IHC, Mouse)	IF/IHC	Mouse
Yamanaka K, Dimitroff CJ, Fuhlbrigge RC et al. Vitamins A and D are potent inhibitors of cutaneous lymphocyte-associated antigen expression. J Allergy Clin Immunol. 2008-01-01 [PMID: 17910894] (WB)	WB
Walcheck B, Leppanen A, Cummings R et al. The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin. Blood. 2002-06-01 [PMID: 12010808] (FLOW, ELISA)	FLOW, ELISA
Borges E, Pendl G, Eytner R et al. The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated. J Biol Chem. 1997-11-01 [PMID: 9353350]
Duijvestijn AM, Horst E, Pals ST et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am J Pathol. 1988-01-01 [PMID: 3276207]
Berg EL, Yoshino T, Rott LS et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J Exp Med. 1991-12-01 [PMID: 1720810]
Picker LJ, Kishimoto TK, Smith CW et al. ELAM-1 is an adhesion molecule for skin-homing T cells. Nature. 1991-02-01 [PMID: 1705666]
Picker LJ, Michie SA, Rott LS, Butcher EC. A unique phenotype of skin-associated lymphocytes in humans. Preferential expression of the HECA-452 epitope by benign and malignant T cells at cutaneous sites. Am J Pathol. 1990-05-01 [PMID: 1693467] (IF/IHC, WB, FLOW)	IF/IHC, WB, FLOW
Show All 12 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IHC-P	Human	10/13/2021	View Summary Application Immunohistochemistry-Paraffin Sample Tested human tonsil Species Human Lot B

Array NB100-78039

• PSGL-1/CD162 Antibodies
• PSGL-1/CD162 ELISA Kit
• PSGL-1/CD162 Lysate
• PSGL-1/CD162 Protein
• PSGL-1/CD162 Proteins and Enzymes
• PSGL-1/CD162 ELISA