![Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, catalog # 5860461; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus catalog # NB600-586) at 1:500 dilution for 1 hour at room temperature followed by incubation with the anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/Von-Willebrand-Factor-Antibody-Dual-RNAscope-ISH-IHC-NB600-586-img0007.jpg "Dual RNAscope ISH-IHC: Von Willebrand Factor Antibody [NB600-586] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for vWF mRNA (ACD RNAScope probe, catalog # 5860461; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus catalog # NB600-586) at 1:500 dilution for 1 hour at room temperature followed by incubation with the anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).")
| Reactivity | Hu, Mu, Ca, FeSpecies Glossary |
| Applications | ICC/IF, IHC, Dual ISH-IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Purified antisera of the Von Willebrand Factor (factor VIII R:ag) related antigen in plasma. |
| Localization | Secreted. Localized to storage granules. |
| Marker | Endothelial Cell Marker |
| Specificity | The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin's or ethanol-fixed and paraffin-embedded tissue sections. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | VWF |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IHC-P: We recommend proteolycic antigen retrieval. After deparaffinization treat with 0.1% Pronase in PBS for 5-10 minutes at 37 degrees C. Alternatively, digest with 0.1% Trypsin in PBS for 25 minutes at 37 degrees C. Gently rinse in PBS. This Von Willebrand Factor Antibody is validated for IHC-Fr from a verified customer review. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Purity | IgG purified |
![Immunohistochemistry CD31/PECAM-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD31_AF3628_Immunohistochemistry_10287.jpg)
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-Fr | Canine | 12/16/2019 |
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reviewed by:
Yukio Sasaki |
IF | Mouse | 01/05/2018 |
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reviewed by:
Verified Customer |
IHC-Fr | Feline | 08/01/2017 |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Von Willebrand Factor Antibody (NB600-586)Find related products by research area.
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Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the... Read full blog post. |
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Von Willebrand Factor: An important mediator and carrier of hemostasis Human Von Willebrand Factor (factor VIII R: Ag) is a 270 kDa multimeric plasma gylcoprotein. Important in the maintenance of hemostasis, it mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation facto... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 12/16/2019 |
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| Application: | IHC-Fr | |
| Species: | Canine |
| Yukio Sasaki 01/05/2018 |
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| Application: | IF | |
| Species: | Mouse |
| Verified Customer 08/01/2017 |
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| Application: | IHC-Fr | |
| Species: | Feline |
![Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.](http://images.novusbio.com/fullsize/Von-Willebrand-Factor-Antibody-Immunohistochemistry-NB600-586-img0001.jpg "Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.")
![Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.](http://images.novusbio.com/fullsize/Von-Willebrand-Factor-Antibody-Immunohistochemistry-Paraffin-NB600-586-img0005.jpg "Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.")
![Immunohistochemistry-Frozen: Von Willebrand Factor Antibody [NB600-586] - Canine brain/spinal cord. VWF antibody in green. A pericyte marker in red. Alexa Fluor conjugated secondary antibodies were used for visualization. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Von-Willebrand-Factor-Antibody-Immunohistochemistry-Frozen-NB600-586-img0008.jpg "Immunohistochemistry-Frozen: Von Willebrand Factor Antibody [NB600-586] - Canine brain/spinal cord. VWF antibody in green. A pericyte marker in red. Alexa Fluor conjugated secondary antibodies were used for visualization. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.](http://images.novusbio.com/fullsize/Von-Willebrand-Factor-Antibody-Immunohistochemistry-Paraffin-NB600-586-img0006.jpg "Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
