CD63 Antibody (H5C6) - BSA Free

CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface in the endosomal system (1-3). More specifically, it is generally present in multivesicular bodies (MVBs), also called late endosomes, and lysosomes (1). The CD63 molecule is a total of 238 amino acids (aa), has four hydrophobic membrane-spanning regions and three N-glycosylation sites, and the encoded protein has a theoretical molecular weight of 25 kDa (2,3). CD63 both directly and indirectly interacts with other proteins including integrins, cell surface receptors, other tetraspanins, kinases, and adapter proteins, to name a few (1). CD63 is involved in many cell processes including cell survival and activation, cell adhesion, invasion, and migration (1). Additionally, CD63 has been shown to interact with tissue inhibitor of metalloproteinase 1 (TIMP-1), which originally thought to be an inhibitor of cancer progression has recently been shown to have cancer promoting properties as well (1,4). The CD63-TIMP-1 interaction has been shown to activate the PI3K/AKT pathway in lung adenocarcinoma cells and also promote survival and invasion of acute myeloid leukemia cells (1).CD63 is a useful flow cytometry marker for basophil granulocytes and can be used for the basophil activation test (BAT) to assess IgE-mediated allergy response (5). As CD63 was initially discovered on activated blood platelets and is a lysosomal-associated protein it makes sense it would be involved in platelet or lysosomal-related disorders (1,2). More precisely, CD63 is associated with Hermansky-Pudlak syndrome, a disease characterized by albinism and platelet storage pool deficiency (6).

References

1. Pols, M. S., & Klumperman, J. (2009). Trafficking and function of the tetraspanin CD63. Experimental cell research. https://doi.org/10.1016/j.yexcr.2008.09.020

2. Metzelaar, M. J., Wijngaard, P. L., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., & Clevers, H. C. (1991). CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. The Journal of biological chemistry.

3. Horejsi, V., & Vlcek, C. (1991). Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. FEBS letters. https://doi.org/10.1016/0014-5793(91)80988-f

4. Eckfeld, C., HauBler, D., Schoeps, B., Hermann, C. D., & Kruger, A. (2019). Functional disparities within the TIMP family in cancer: hints from molecular divergence. Cancer metastasis reviews. https://doi.org/10.1007/s10555-019-09812-6

5. Hoffmann, H. J., Santos, A. F., Mayorga, C., Nopp, A., Eberlein, B., Ferrer, M., Rouzaire, P., Ebo, D. G., Sabato, V., Sanz, M. L., Pecaric-Petkovic, T., Patil, S. U., Hausmann, O. V., Shreffler, W. G., Korosec, P., & Knol, E. F. (2015). The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease. Allergy. https://doi.org/10.1111/all.12698

6. Dell'Angelica, E. C., Shotelersuk, V., Aguilar, R. C., Gahl, W. A., & Bonifacino, J. S. (1999). Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. Molecular cell. https://doi.org/10.1016/s1097-2765(00)80170-7

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 12 applications: B/N, Cytometric Bead Assay Standard, DB, ELISA, EM, FLOW, Func, IA, ICC/IF, IP, In vitro, WB.

Showing Publications 1 - 10 of 53. Show All 53 Publications. Collapse Publications.

Publications using NBP2-42225	Applications	Species
Kim S, Lee K, Choi YS et al. Mitochondrial double-stranded RNAs govern the stress response in chondrocytes to promote osteoarthritis development Cell reports 2022-08-09 [PMID: 35947956] (WB) Details: Supplementary Figure S5.	WB
Saftics A, Abuelreich S, Romano E et al. Single Extracellular VEsicle Nanoscopy Journal of extracellular vesicles 2023-07-01 [PMID: 37422692] (DB, Human) Details: 1:500 dilution	DB	Human
Wang J, Trau M, Wuethrich A A Microfluidic SERS Assay to Characterize the Phenotypic Heterogeneity in Cancer-Derived Small Extracellular Vesicles Methods in molecular biology (Clifton, N.J.) 2023-06-10 [PMID: 37300621]
Bernuz M, Pallares-Rusinol A, Rossi R et al. Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications Methods in molecular biology (Clifton, N.J.) 2023-05-04 [PMID: 37140802]
Del Rivero T, Milberg J, Bennett C et al. Human amniotic fluid derived extracellular vesicles attenuate T cell immune response Frontiers in immunology 2022-11-28 [PMID: 36518766] (ICC/IF, Human)	ICC/IF	Human
de la Cruz-Ojeda P, Schmid T, Boix L et al. miR-200c-3p, miR-222-5p, and miR-512-3p Constitute a Biomarker Signature of Sorafenib Effectiveness in Advanced Hepatocellular Carcinoma Cells 2022-08-28 [PMID: 36078082] (WB, Human)	WB	Human
Ali Moussa HY, Manaph N, Ali G et al. Single Extracellular Vesicle Analysis Using Flow Cytometry for Neurological Disorder Biomarkers Frontiers in integrative neuroscience 2022-05-17 [PMID: 35655952] (WB, Human) Details: Dilutions: 1:1000	WB	Human
Lin W, Yuan L, Gao Z et al. An Integrated Sample-to-Answer SERS Platform for Multiplex Phenotyping of Extracellular Vesicles SSRN Electronic Journal 2023-01-28
Su C, Lai Y, Bamodu O et al. Cancer-Associated Fibroblasts Release Exosomal CBFBThat Dictate an Aggressive Bone Metastasis Phenotype in Breast Cancer Research Square Dec 29 2020 (WB, Human)	WB	Human
Matsui T, Sakamaki Y, Hiragi S, Fukuda M VAMP5 and distinct sets of cognate Q-SNAREs mediate exosome release Cell structure and function 2023-09-14 [PMID: 37704453]
Wright AB The isolation, culture-expansion, cryopreservation, characterization, and properties of umbilical cord-derived mesenchymal stromal cells and their extracellular vesicles Thesis 2021-01-01
Gobin J Assessment of High Purity Mesenchymal Stromal Cells Derived Extracellular Vesicles Presenting NRP1 Show Functional Suppression of Activated Immune Cells Thesis Jan 1 2022 (IP, Human)	IP	Human
Proestler E, Donzelli J, Nevermann S et al. The multiple functions of miR-574-5p in the neuroblastoma tumor microenvironment Frontiers in pharmacology 2023-09-04 [PMID: 37731742] (WB, Human)	WB	Human
Hsu CH, Ma HP, Ong JR et al. Cancer-Associated Exosomal CBFB Facilitates the Aggressive Phenotype, Evasion of Oxidative Stress, and Preferential Predisposition to Bone Prometastatic Factor of Breast Cancer Progression Disease markers 2022-07-19 [PMID: 35903297]
Conkright WR, Beckner ME, Sterczala AJ et al. Resistance Exercise Differentially Alters Extracellular Vesicle Size and Subpopulation Characteristics in Healthy Men and Women: An Observational Cohort Study Physiological genomics 2022-07-11 [PMID: 35816651] (FLOW, Human)	FLOW	Human
Lee C, Chen Y, Hsiao A Et al. Demystifying the long noncoding RNA landscape of small EVs derived from human mesenchymal stromal cells J Adv Res 2022-07-01 [PMID: 35777918]
Fleming Martinez A, DOppler H, Bastea L et al. Ym1+ macrophages orchestrate fibrosis, lesion growth and progression during development of murine pancreatic cancer iScience 2022-04-01 [PMID: 35602933] (B/N, Mouse)	B/N	Mouse
Dou X, Hua Y, Chen Z et al. Extracellular Vesicles Containing PD-L1 Contribute to CD8 + T-Cell Immune Suppression and Predict Poor Outcomes in Small Cell Lung Cancer Clin Exp Immunol 2022-05-13 [PMID: 35553630]
Beckner ME, Conkright WR, Sahu A Et al. Utility of extracellular vesicles as a potential biological indicator of physiological resilience during military operational stress Physiol Rep 2022-04-04 [PMID: 35373929] (ICC/IF, Human) Details: Citation using the Alexa Fluor 700 version of this antibody.	ICC/IF	Human
Kolka CM, Webster J, Lepletier A et al. C5b-9 Membrane Attack Complex Formation and Extracellular Vesicle Shedding in Barrett's Esophagus and Esophageal Adenocarcinoma Frontiers in immunology 2022-03-08 [PMID: 35345676] (IA, Human)	IA	Human
Conkright WR, Beckner ME, Sahu A et al. Men and Women Display Distinct Extracellular Vesicle Biomarker Signatures in Response to Military Operational Stress Journal of applied physiology (Bethesda, Md. : 1985) 2022-03-17 [PMID: 35297690]
Rudy MJ, Coughlan C, Hixon AM et al. Density Analysis of Enterovirus D68 Shows Viral Particles Can Associate with Exosomes Microbiology spectrum 2022-02-23 [PMID: 35170992] (IP, Human)	IP	Human
Li W, Xu J, Cheng L et al. RelB promotes the migration and invasion of prostate cancer DU145 cells via exosomal ICAM1 in vitro Cellular signalling 2021-12-18 [PMID: 34933092] (WB, Human)	WB	Human
Leroux E, Perbet R, Caillierez R Et al. Extracellular vesicles: Major actors of heterogeneity in tau spreading among human tauopathies Molecular therapy : the journal of the American Society of Gene Therapy 2021-09-24 [PMID: 34563677] (EM, Human)	EM	Human
Bellio MA, Young KC, Milberg J et al. Amniotic fluid-derived extracellular vesicles: characterization and therapeutic efficacy in an experimental model of bronchopulmonary dysplasia Cytotherapy 2021-09-17 [PMID: 34538718] (ICC/IF)	ICC/IF
Lee J, Lee SA, Gu NY et al. Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor BioMed research international 2021-09-04 [PMID: 34527741] (WB, Canine)	WB	Canine
Teshima T, Yuchi Y, Suzuki R Et al. Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4+ T Cells Isolated from Peripheral Blood Mononuclear Cells Journal of immunology research 2021-08-14 [PMID: 34447855]
Wang J, Wuethrich A, Lobb RJ et al. Characterizing the Heterogeneity of Small Extracellular Vesicle Populations in Multiple Cancer Types via an Ultrasensitive Chip ACS sensors 2021-07-15 [PMID: 34264628]
Pederson PJ, Liang H, Filonov D, Mooberry SL. Eribulin and Paclitaxel Differentially Alter Extracellular Vesicles and Their Cargo from Triple-Negative Breast Cancer Cells Cancers (Basel) 2021-07-02 [PMID: 34205051] Details: This citation used the Alexa Fluor 488 format of this antibody.
Krishnamachary B, Mahajan A, Kumar A et al. Extracellular vesicle TGF-beta 1 is linked to cardiopulmonary dysfunction in HIV American journal of respiratory cell and molecular biology 2021-05-20 [PMID: 34014809]
Arab T, Mallick ER, Huang Y et al. Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms Journal of extracellular vesicles 2021-04-01 [PMID: 33850608]
Tomishige N, Murate M, Didier P et al. The use of pore-forming toxins to image lipids and lipid domains Methods in Enzymology 2021-02-26 [PMID: 33712198]
Zhou J, Wu Z, Hu J et al High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations Sci Adv 2020-11-21 [PMID: 33219024] (ICC/IF, Human) Details: Citation using the Alexa Fluor 488 version of this antibody.	ICC/IF	Human
Wang J, Wuethrich A, Sina AA et al. Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma Sci Adv 2020-02-01 [PMID: 32133394] (WB, Human)	WB	Human
Kumar SR, Kimchi ET, Manjunath Y et al. RNA cargos in extracellular vesicles derived from blood serum in pancreas associated conditions Sci Rep 2020-02-18 [PMID: 32071328] (WB, Human)	WB	Human
An J H, Li Q et al. TSG-6 in extracellular vesicles from canine mesenchymal stem/stromal is a major factor in relieving DSS-induced colitis. PLoS One 2020-11-02 [PMID: 32040478] (WB, Canine)	WB	Canine
Zeng X, Carlin CR Adenovirus early region 3 RID alpha protein limits NF kappa B signaling through stress-activated EGF receptors PLoS Pathog. 2019-08-01 [PMID: 31425554]
Cui, X, Liu, Y Et al. Circulating Exosomes Activate Dendritic Cells and Induce Unbalanced CD4+ T Cell Differentiation in Hashimoto Thyroiditis. J Clin Endocrinol Metab 2019-10-01 [PMID: 31199456] (FLOW, Rat)	FLOW	Rat
Kuo HF, Hsieh CC, Wang SC et al. Simvastatin Attenuates Cardiac Fibrosis via Regulation of Cardiomyocyte-Derived Exosome Secretion J Clin Med 2019-06-04 [PMID: 31167519] (WB, Human)	WB	Human
Huang WT, Chong IW, Chen HL et al. Pigment epithelium-derived factor inhibits lung cancer migration and invasion by upregulating exosomal thrombospondin 1. Cancer Lett. 2018-11-12 [PMID: 30439539] (WB, Human)	WB	Human
Rafat N Theoretical and Experimental Studies of Multistep Electrochemical Biosensors ACS Nano 2018-02-24 [PMID: 29474060]
Bilawchuk LM, Griffiths CD, Jensen LD et al. The Susceptibilities of Respiratory Syncytial Virus to Nucleolin Receptor Blocking and Antibody Neutralization are Dependent upon the Method of Virus Purification Viruses 2017-08-03 [PMID: 28771197] (WB, Human)	WB	Human
Green LR, Monk PN, Partridge LJ, Morris Pet al. Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.. Infect Immun. 2011-06-01 [PMID: 21464080] (ICC/IF, In vitro, Human)	ICC/IF, In vitro	Human
Parthasarathy V, Martin F, Higginbottom A et al. Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.. Immunology. 2009-06-01 [PMID: 19489128] (Func, In vitro, FLOW, WB, Human) Details: CD63 antibody (clone H5C6) was used for FLOW application on human peripheral blood monocytes which were isolated from peripheral whole blood of healthy volunteers and the assay implicated - culture of adherent monocytes in the presence or absence of 10 ug/ml of Con A lectin, harvesting followed by washing with B/B/N buffer ( balanced salt solution/BSS with 0·2% BSA and 0·1% azide), incubation of 2 × 10^5 cells with 10ug of primary antibody or IgG1 isotype control on ice for 45 minutes, 2X washing with B/B/N buffer followed by 30 minutes incubation with FITC-labelled anti-mouse immunoglobulin , washing and analysis on flow cytometer (data not shown). The antibody was also used in functional/in-vitro assay for analysing the effects of anti-tetraspanin immunoglobulins on concanavalin A (Con A) induced monocyte fusion / multinucleated giant cells formation (Figure 2 and Figure 3), and for WB analysis of tetraspanin recombinant protein- extracellular domain-GST (Figure S1).	Func, In vitro, FLOW, WB	Human
Tang Y, Leao IC, Coleman EM et al. Deficiency of niemann-pick type C-1 protein impairs release of human immunodeficiency virus type 1 and results in Gag accumulation in late endosomal/lysosomal compartments. J Virol 2009-08-01 [PMID: 19474101] (ICC/IF, Human)	ICC/IF	Human
Nydegger S, Khurana S, Krementsov DN et al. Mapping of tetraspanin-enriched microdomains that can function as gateways for HIV-1.. J Cell Biol. 2006-06-05 [PMID: 16735575] (ICC/IF, EM, Human)	ICC/IF, EM	Human
Nguyen DG, Booth A, Gould SJ, Hildreth JE. Evidence that HIV budding in primary macrophages occurs through the exosome release pathway.. J Biol Chem. 2003-12-26 [PMID: 14561735] (FLOW, ELISA, Cytometric Bead Assay Standard, Human)	FLOW, ELISA, Cytometric Bead Assay Standard	Human
Klein-Soyer C, Azorsa DO, Cazenave JP, Lanza F. CD9 participates in endothelial cell migration during in vitro wound repair.. Arterioscler Thromb Vasc Biol. 2000-02-01 [PMID: 10669631] (Func, FLOW, ICC/IF, In vitro, Human)	Func, FLOW, ICC/IF, In vitro	Human
Azorsa DO, Moog S, Cazenave JP, Lanza F. A general approach to the generation of monoclonal antibodies against members of the tetraspanin superfamily using recombinant GST fusion proteins.. J Immunol Methods. 1999-10-29 [PMID: 10556689] (ELISA, WB)	ELISA, WB
Hildreth JE. Syncytium-inhibiting monoclonal antibodies produced against human T-cell lymphotropic virus type 1-infected cells recognize class II major histocompatibility complex molecules and block by protein crowding.. J Virol. 1998-12-01 [PMID: 9811687] (Func, Human)	Func	Human
Azorsa DO, Hyman JA, Hildreth JE. CD63/Pltgp40: a platelet activation antigen identical to the stage-specific, melanoma-associated antigen ME491.. Blood. 1991-07-15 [PMID: 2070066] (IP, Human)	IP	Human
Hildreth JE, Derr D, Azorsa DO et al. Characterization of a novel self-associating Mr 40,000 platelet glycoprotein.. Blood. 1991-01-01 [PMID: 1984792] (IP, WB, FLOW, Human)	IP, WB, FLOW	Human
Show All 53 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	2 reviewed by: Verified Customer	ICC	Canine	09/01/2022	View Summary Application Immunocytochemistry Sample Tested MDCK canine kidney epithelial cell line Species Canine Lot C-9
Enlarge	5 reviewed by: Verified Customer	DB	Canine	10/09/2020	View Summary Application Dot Blot Sample Tested cell lysate canine umbilical cord-derived mesenchymal stromal cells Species Canine Lot C-2
Enlarge	3 reviewed by: Verified Customer	WB	Human	10/02/2019	View Summary Application Western Blot Sample Tested Hela whole cell lysate Species Human Lot B-4
Enlarge	5 reviewed by: Verified Customer	WB	Human	09/18/2017	View Summary Application Western Blot Sample Tested Blood plasma,Urine Species Human Lot A-1 Comments Comments Antibody labelled with HRP
Enlarge	3 reviewed by: Sneha Bhat	Immunocytochemistry/Immunofluorescence	Human	11/01/2016	View Summary Application Immunocytochemistry/Immunofluorescence Sample Tested HEK cells Species Human
Enlarge	4 reviewed by: Sneha Bhat	Immunocytochemistry/Immunofluorescence	Canine	11/01/2016	View Summary Application Immunocytochemistry/Immunofluorescence Sample Tested Madin-Darby Canine Kidney Epithelial Cells (MDCK Line) Species Canine

Array NBP2-42225

• CD63 Antibodies
• CD63 ELISA Kit
• CD63 Lysate
• CD63 Proteins
• CD63 Proteins and Enzyme