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P-Selectin/CD62P Antibody (Psel.KO.2.7) [Allophycocyanin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP
Clone
Psel.KO.2.7
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

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P-Selectin/CD62P Antibody (Psel.KO.2.7) [Allophycocyanin] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
P-selectin transfected 300.19 cells
Localization
Membrane; single pass type I membrane protein.
Specificity
NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SELP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Reviewed Applications
Read 1 Review rated 5
using
NB100-65392APC in the following applications:

Publications
Read Publication using
NB100-65392APC in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for P-Selectin/CD62P Antibody (Psel.KO.2.7) [Allophycocyanin]

  • CD62P antigen
  • CD62P
  • FLJ45155
  • GMP140
  • GRMP
  • PADGEM
  • PADGEMantigen CD62)
  • PSEL
  • P-Selectin
  • selectin P (granule membrane protein 140kDa, antigen CD62)
  • SELP

Background

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P-Selectin/CD62P Antibody (NB100-65392APC)(1)

We have publications tested in 1 confirmed species: Equine.

We have publications tested in 1 application: FLOW.


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FLOW
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Review for P-Selectin/CD62P Antibody (NB100-65392APC) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB100-65392APC:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
Flow Other 06/17/2015
View

Summary

ApplicationFlow Cytometry
SpeciesOther

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for P-Selectin/CD62P Antibody (NB100-65392APC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

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Recent Reviews

5
5
1
4
0
3
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2
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1
0

 
Verified Customer
06/17/2015
Application: Flow
Species: Other

Bioinformatics

Gene Symbol SELP