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ICAM-1/CD54 Antibody [Unconjugated]

Name: ICAM-1/CD54 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF796
Rating: 4.5 (6 reviews)

(88 Publications)

(6 Reviews)

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ICAM-1/CD54 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Increased stress kinase signaling and JNK pathway-dependent cytokine and chemokine production by primary keratinocytes lacking BRAF and RAF1.(A) Reduced ERK phosphorylation and increased JNK/p38 activation in primary ...read more

D-JNKI1 treatment rescues inflammation in delta / delta ep2 mice.Mice were treated with D-JNKI1 or TAT peptide (22 mg/kg i.p. at 10 days of age) and analyzed after 12 days (A) D-JNKI1 treatment prevents disease onset ...read more

Product Details

Summary

Reactivity	MuSpecies Glossary
Applications	WB, IHC, B/N
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

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Catalog# & Conjugate	Size	Price
Biotin BAF796

View Available Formulations

Catalog# & Formulation	Size	Price
Carrier Free AF796-SP

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Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF796

ICAM-1/CD54 Antibody [Unconjugated] Summary

Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1/CD54 Gln28-Asn485 Accession # Q3U8M7
Specificity	Detects mouse ICAM-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat ICAM-1 is observed, approximately 5% cross-reactivity with recombinant human (rh) ICAM-1 and rhICAM-3 is observed, and less than 1% cross-reactivity with recombinant mouse ICAM-2 is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	ICAM1
Purity Statement	Antigen Affinity-purified
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Adhesion Blockade
Immunohistochemistry 5-15 ug/mL
Western Blot 0.1 ug/mL

Reviewed Applications

Read 6 Reviews rated 4.5

using
AF796 in the following applications:

Immunohistochemistry-Frozen
Immunohistochemistry
Immunofluorescence
Western Blot

Publications

Read Publications using
AF796 in the following applications:

ICC
1 publication
IHC
21 publications
IHC-Fr
8 publications
IHC-P
6 publications
Immunohistochemistry
4 publications
Neutralization
1 publication
Western Blot
21 publications

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody [Unconjugated]

BB2
CD54 antigen
CD54
cell surface glycoprotein P3.58
human rhinovirus receptor
ICAM1
ICAM-1
intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
intercellular adhesion molecule 1
Major group rhinovirus receptor
P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta , and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
Siu, G. et al. (1989) J. Immunol. 143:3813.
Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICAM-1/CD54
Antibody [Unconjugate

AF796

Species: Mu

Applications: WB, IHC, B/N

Publications for ICAM-1/CD54 Antibody (AF796)(88)

We have publications tested in 4 confirmed species: Human, Mouse, Hamster, Transgenic Mouse.

We have publications tested in 7 applications: ICC, IHC, IHC-Fr, IHC-P, Immunohistochemistry, Neutralization, Western Blot.

Showing Publications 1 - 10 of 88. Show All 88 Publications.

Publications using AF796	Applications	Species
Ren, W;Li, W;Cha, X;Wang, S;Cai, B;Wang, T;Li, F;Li, T;Xie, Y;Xu, Z;Wang, Z;Liu, H;Yu, Y; Single-cell transcriptomic atlas of taste papilla aging Aging cell 2024-08-21 [PMID: 39169434] (Immunohistochemistry, Mouse)	Immunohistochemistry	Mouse
Angolano, C;Hansen, E;Ajjawi, H;Nowlin, P;Zhang, Y;Thunemann, N;Ferran, C;Todd, N; Characterization of focused ultrasound blood-brain barrier disruption effect on inflammation as a function of treatment parameters bioRxiv : the preprint server for biology 2024-07-16 [PMID: 39071338] (Immunohistochemistry, Western Blot, Mouse)	Immunohistochemistry, Western Blot	Mouse
Manandhar, S;Gaddam, RR;Chambers, S;Bhatia, M; Kupffer Cell Inactivation Alters Endothelial Cell Adhesion Molecules in Cecal Ligation and Puncture-Induced Sepsis Biomolecules 2024-01-09 [PMID: 38254684] (IHC, Mouse)	IHC	Mouse
Yin, D;Wang, C;Qi, Y;Wang, YC;Hagemann, N;Mohamud Yusuf, A;Dzyubenko, E;Kaltwasser, B;Tertel, T;Giebel, B;Gunzer, M;Popa-Wagner, A;Doeppner, TR;Hermann, DM; Neural precursor cell delivery induces acute post-ischemic cerebroprotection, but fails to promote long-term stroke recovery in hyperlipidemic mice due to mechanisms that include pro-inflammatory responses associated with brain hemorrhages Journal of neuroinflammation 2023-09-15 [PMID: 37715288] (IHC, Mouse)	IHC	Mouse
Manandhar, S;Chambers, S;Miller, A;Ishii, I;Bhatia, M; Pharmacological Inhibition and Genetic Deletion of Cystathionine Gamma-Lyase in Mice Protects against Organ Injury in Sepsis: A Key Role of Adhesion Molecules on Endothelial Cells International journal of molecular sciences 2023-09-04 [PMID: 37686458] (IHC, Transgenic Mouse, Mouse)	IHC	Transgenic Mouse, Mouse
Yu, L;Xu, L;Chu, H;Peng, J;Sacharidou, A;Hsieh, HH;Weinstock, A;Khan, S;Ma, L;Durán, JGB;McDonald, J;Nelson, ER;Park, S;McDonnell, DP;Moore, KJ;Huang, LJ;Fisher, EA;Mineo, C;Huang, L;Shaul, PW; Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice Nature communications 2023-07-25 [PMID: 37491347] (IHC, Mouse)	IHC	Mouse
Anderson R. A. Cerqueira, Leandro Rodrigues, Silvia Abigail Coavoy-Sánchez, Simone A. Teixeira, Karla B. Feitosa, Erika Y. Taniguchi et al. Aedes aegypti salivary gland extract alleviates acute itching by blocking TRPA1 channels Frontiers in Physiology 6/27/2023 [PMID: 37441000]
Cornuault, L;Hérion, FX;Bourguignon, C;Rouault, P;Foussard, N;Alzieu, P;Chapouly, C;Gadeau, AP;Couffinhal, T;Renault, MA; Partial Mural Cell Ablation Disrupts Coronary Vasculature Integrity and Induces Systolic Dysfunction Journal of the American Heart Association 2023-06-22 [PMID: 37345826] (Immunohistochemistry, Western Blot, Transgenic Mouse)	Immunohistochemistry, Western Blot	Transgenic Mouse
Kim, JY;Yi, MH;Kim, M;Choi, JH;Lee, S;Yong, TS; Production of Dermatophagoides farinae Having Low Bacterial Content Using Ampicillin Journal of immunology research 2023-05-18 [PMID: 37252681] (Immunohistochemistry, Mouse)	Immunohistochemistry	Mouse
Markos Patsouras, Eirini Alexopoulou, Spyros Foutadakis, Eirini Tsiki, Panagiota Karagianni, Marios Agelopoulos et al. Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells Journal of Translational Autoimmunity 5/2/2023 [PMID: 37216142]
Z Ma, W Li, L Zhuang, T Wen, P Wang, H Yu, Y Liu, Y Yu TMEM59 ablation leads to loss of olfactory sensory neurons and impairs olfactory functions via interaction with inflammation Brain, Behavior, and Immunity, 2023-04-13;111(0):151-168. 2023-04-13 [PMID: 37061103] (IHC, Mouse)	IHC	Mouse
Hyun-Jin An, Mi-Gyeong Gwon, Hyemin Gu, Seongjae Bae, Jaechan Leem, Jin Bae Lee et al. STAT3/NF‑ kappa B decoy oligodeoxynucleotides inhibit atherosclerosis through regulation of the STAT/NF‑ kappa B signaling pathway in a mouse model of atherosclerosis International Journal of Molecular Medicine 3/31/2023 [PMID: 37026512]
Yuanyuan Zhang, Tianxiao Liu, Zhiyong Deng, Wenqian Fang, Xian Zhang, Shuya Zhang et al. Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils Advanced Science 3/1/2023 [PMID: 36592421]
Rebeca Uceda-Castro, Andreia S. Margarido, Lesley Cornet, Serena Vegna, Kerstin Hahn, Ji-Ying Song et al. Re-purposing the pro-senescence properties of doxorubicin to introduce immunotherapy in breast cancer brain metastasis Cell Reports Medicine 11/15/2022 [PMID: 36384097]
W Ren, Z Ma, L Wang, X Feng, H Yu, Y Yu Lgr5+ cells are required and dynamically participate in olfactory epithelium regeneration: a revisiting shows Lgr5 expression in multiple cell lineages Theranostics, 2022-07-18;12(13):5631-5644. 2022-07-18 [PMID: 35966594] (IHC, Mouse)	IHC	Mouse
L Liu, C Yang, BP Lavayen, RJ Tishko, J Larochelle, E Candelario Targeted BRD4 protein degradation by dBET1 ameliorates acute ischemic brain injury and improves functional outcomes associated with reduced neuroinflammation and oxidative stress and preservation of blood-brain barrier integrity Journal of Neuroinflammation, 2022-06-27;19(1):168. 2022-06-27 [PMID: 35761277] (IHC, Mouse)	IHC	Mouse
Hinami Asano, Sanae Hasegawa-Ishii, Ken Arae, Aki Obara, Geoffroy Laumet, Robert Dantzer et al. Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammation Journal of Neuroimmunology 7/1/2022 [PMID: 35661951]
Y Jin, Y Liu, L Xu, J Xu, Y Xiong, Y Peng, K Ding, S Zheng, N Yang, Z Zhang, L Li, L Tan, H Song, J Fu Novel role for caspase 1 inhibitor VX765 in suppressing NLRP3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosis Cell Death & Disease, 2022-05-31;13(5):512. 2022-05-31 [PMID: 35641492] (IHC, Mouse)	IHC	Mouse
YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764. 2022-03-11 [PMID: 35360491] (Western Blot, Mouse)	Western Blot	Mouse
C Huisman, MA Norgard, PR Levasseur, SM Krasnow, MGP van der Wi, B Olson, DL Marks Critical changes in hypothalamic gene networks in response to pancreatic cancer as found by single-cell RNA sequencing Molecular Metabolism, 2022-01-11;0(0):101441. 2022-01-11 [PMID: 35031523] (IHC, Mouse)	IHC	Mouse
Calvier L, Manouchehri N, Sacharidou A et al. Apolipoprotein E receptor 2 deficiency decreases endothelial adhesion of monocytes and protects against autoimmune encephalomyelitis Science Immunology 2021-08-27 [PMID: 34452924]
Calvier L, Manouchehri N, Sacharidou A et al. Apolipoprotein E receptor 2 deficiency decreases endothelial adhesion of monocytes and protects against autoimmune encephalomyelitis Science Immunology 8/27/2021 [PMID: 34452924]
Laurent Calvier, Xunde Xian, Richard G. Lee, Anastasia Sacharidou, Chieko Mineo, Philip W. Shaul et al. Reelin Depletion Protects Against Atherosclerosis by Decreasing Vascular Adhesion of Leukocytes Arteriosclerosis, Thrombosis, and Vascular Biology 4/1/2021 [PMID: 33626909]
Nina Hagemann, Ayan Mohamud Yusuf, Carlotta Martiny, Xiaoni Zhang, Christoph Kleinschnitz, Matthias Gunzer et al. Homozygous Smpd1 deficiency aggravates brain ischemia/ reperfusion injury by mechanisms involving polymorphonuclear neutrophils, whereas heterozygous Smpd1 deficiency protects against mild focal cerebral ischemia Basic Research in Cardiology 12/1/2020 [PMID: 33057972]
SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm Exp. Mol. Med., 2020-09-14;0(0):. 2020-09-14 [PMID: 32929220] (Western Blot, Mouse)	Western Blot	Mouse
B Dufek, DT Meehan, D Delimont, K Wilhelm, G Samuelson, R Coenen, J Madison, E Doyle, B Smyth, G Phillips, MA Gratton, D Cosgrove RNA-seq analysis of gene expression profiles in isolated stria vascularis from wild-type and Alport mice reveals key pathways underling Alport strial pathogenesis PLoS ONE, 2020-08-21;15(8):e0237907. 2020-08-21 [PMID: 32822386] (IHC, Mouse)	IHC	Mouse
CM Chen, ML Wu, YC Ho, PY Gung, MH Tsai, AN Orekhov, IA Sobenin, P Lin, SF Yet Exposure to Zinc Oxide Nanoparticles Disrupts Endothelial Tight and Adherens Junctions and Induces Pulmonary Inflammatory Cell Infiltration Int J Mol Sci, 2020-05-13;21(10):. 2020-05-13 [PMID: 32414036] (Western Blot, Human)	Western Blot	Human
PY Hsueh, Y Ju, A Vega, MC Edman, JA MacKay, SF Hamm-Alvar A Multivalent ICAM-1 Binding Nanoparticle which Inhibits ICAM-1 and LFA-1 Interaction Represents a New Tool for the Investigation of Autoimmune-Mediated Dry Eye Int J Mol Sci, 2020-04-15;21(8):. 2020-04-15 [PMID: 32326657] (IHC, Mouse)	IHC	Mouse
Marcus Ruscetti, John P. Morris, Riccardo Mezzadra, James Russell, Josef Leibold, Paul B. Romesser et al. Senescence-Induced Vascular Remodeling Creates Therapeutic Vulnerabilities in Pancreas Cancer Cell 4/1/2020 [PMID: 32234521]
Tai-Ling Chao, Sing-Yi Gu, Pi-Han Lin, Yu-Tien Chou, Thai-Yen Ling, Sui-Yuan Chang Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells Frontiers in Microbiology 1/21/2020 [PMID: 32038512]
Dániel P Varga, Ákos Menyhárt, Balázs Pósfai, Eszter Császár, Nikolett Lénárt, Csaba Cserép et al. Microglia alter the threshold of spreading depolarization and related potassium uptake in the mouse brain Journal of Cerebral Blood Flow & Metabolism 12/1/2020 [PMID: 31987008]
S Colijn, V Muthukumar, J Xie, S Gao, CT Griffin Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis Dis Model Mech, 2020-01-24;13(1):. 2020-01-24 [PMID: 31953345] (Western Blot, Mouse)	Western Blot	Mouse
W Peng, GQ Pei, Y Tang, L Tan, W Qin IgA1 deposition may induce NLRP3 expression and macrophage transdifferentiation of podocyte in IgA nephropathy J Transl Med, 2019-12-03;17(1):406. 2019-12-03 [PMID: 31796125] (Western Blot, Mouse)	Western Blot	Mouse
KE Salmeron, ME Maniskas, DN Edwards, R Wong, I Rajkovic, A Trout, AA Rahman, S Hamilton, JF Fraser, E Pinteaux, GJ Bix Interleukin 1 alpha administration is neuroprotective and neuro-restorative following experimental ischemic stroke J Neuroinflammation, 2019-11-14;16(1):222. 2019-11-14 [PMID: 31727174] (IHC, Mouse)	IHC	Mouse
Knackstedt S L, Georgiadou A et al. Neutrophil extracellular traps drive inflammatory pathogenesis in malaria. Sci Immunol 10/18/2019 [PMID: 31628160]
J Riegler, H Gill, A Ogasawara, M Hedehus, V Javinal, J Oeh, GZ Ferl, J Marik, S Williams, D Sampath, J Schartner, RAD Carano VCAM-1 Density and Tumor Perfusion Predict T-cell Infiltration and Treatment Response in Preclinical Models Neoplasia, 2019-09-11;21(10):1036-1050. 2019-09-11 [PMID: 31521051] (Neutralization, Mouse)	Neutralization	Mouse
Ian C. Han, Erin R. Burnight, Mallory J. Ulferts, Kristan S. Worthington, Stephen R. Russell, Elliott H. Sohn et al. Helper-Dependent Adenovirus Transduces the Human and Rat Retina but Elicits an Inflammatory Reaction When Delivered Subretinally in Rats Human Gene Therapy 11/1/2019 [PMID: 31456426]
Mike R. Wilson, Jake J. Reske, Jeanne Holladay, Genna E. Wilber, Mary Rhodes, Julie Koeman et al. ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion Nature Communications 8/7/2019 [PMID: 31391455]
Guo Xiangjiang, Fereydooni Arash, Isaji Toshihiko et al. Inhibition of the Akt1-mTORC1 Axis Alters Venous Remodeling to Improve Arteriovenous Fistula Patency. Scientific Reports 7/30/2019 [PMID: 31363142]
Xiaoyu Liu, Daniel P. Nemeth, Daniel B. McKim, Ling Zhu, Damon J. DiSabato, Olimpia Berdysz et al. Cell-Type-Specific Interleukin 1 Receptor 1 Signaling in the Brain Regulates Distinct Neuroimmune Activities Immunity 2/1/2019 [PMID: 30683620]
P Wohlrab, L Soto-Gonza, T Benesch, MP Winter, IM Lang, K Markstalle, V Tretter, KU Klein Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells Front Physiol, 2018-12-06;9(0):1754. 2018-12-06 [PMID: 30574096] (Western Blot, Mouse)	Western Blot	Mouse
Wong R, Lenart N, Hill L et al. Interleukin-1 mediates ischaemic brain injury via distinct actions on endothelial cells and cholinergic neurons. Flow Cytometry Basics for the Non-Expert. 11/9/2018 [PMID: 30453020]
V Holler, V Buard, T Roque, C Squiban, M Benderitte, S Flamant, R Tamarat Early and Late Protective Effect of Bone Marrow Mononuclear Cell Transplantation on Radiation-Induced Vascular Dysfunction and Skin Lesions Cell Transplant, 2018-11-09;0(0):9636897188103. 2018-11-09 [PMID: 30409036] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Li Z, Luo T, Ning X, Xiong C, Wu A Neurokinin-1 receptor antagonism improves postoperative neurocognitive disorder in mice. Neurosci Lett, 2018-09-28;687(0):189-195. 2018-09-28 [PMID: 30273701] (IHC, Mouse)	IHC	Mouse
C Xiong, J Liu, D Lin, J Zhang, N Terrando, A Wu Complement activation contributes to perioperative neurocognitive disorders in mice J Neuroinflammation, 2018-09-04;15(1):254. 2018-09-04 [PMID: 30180861] (IHC, Mouse)	IHC	Mouse
A Niraula, Y Wang, JP Godbout, JF Sheridan Corticosterone Production during Repeated Social Defeat Causes Monocyte Mobilization from the Bone Marrow, Glucocorticoid Resistance and Neurovascular Adhesion Molecule Expression J. Neurosci., 2018-01-30;0(0):. 2018-01-30 [PMID: 29382712] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Y Xu, S Xu, P Liu, M Koroleva, S Zhang, S Si, ZG Jin Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis J Am Heart Assoc, 2017-11-30;6(12):. 2017-11-30 [PMID: 29191808] (Western Blot, Mouse)	Western Blot	Mouse
L Ruiz-Remol, C Ollauri-Ib, L Pérez-Roqu, E Núñez-Góme, F Pérez-Barr, JM López-Novo, M Pericacho, A Rodríguez- Circulating soluble endoglin modifies the inflammatory response in mice PLoS ONE, 2017-11-16;12(11):e0188204. 11/16/2017 [PMID: 29145462]
Z Meng, W Klinngam, MC Edman, SF Hamm-Alvar Interferon-? treatment in vitro elicits some of the changes in cathepsin S and antigen presentation characteristic of lacrimal glands and corneas from the NOD mouse model of Sjï¿½gren's Syndrome PLoS ONE, 2017-09-13;12(9):e0184781. 2017-09-13 [PMID: 28902875] (IHC-Fr, Mouse)	IHC-Fr	Mouse
CC Wei, YY Kong, GQ Li, YF Guan, P Wang, CY Miao Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway Sci Rep, 2017-04-06;7(1):717. 2017-04-06 [PMID: 28386082] (IHC, Mouse)	IHC	Mouse
KK Gupta, DL Donahue, MJ Sandoval-C, FJ Castellino, VA Ploplis Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation Sci Rep, 2017-03-23;7(1):365. 2017-03-23 [PMID: 28336948] (Western Blot, Mouse)	Western Blot	Mouse
Epidermal RAF prevents allergic skin disease Elife, 2016-07-19;5(0):. 2016-07-19 [PMID: 27431613] (IHC-P, Mouse)	IHC-P	Mouse
Jia-Xin Shi, Jia-Shu Li, Rong Hu, Xiao-Min Li, Hong Wang CAF1-knockout mice are more susceptive to lipopolysaccharide-induced acute lung injury Journal of Inflammation Research 7/1/2016 [PMID: 27358572]
Christopher N. Hansen, Diana M. Norden, Timothy D. Faw, Rochelle Deibert, Eric S. S.Wohleb, John F. Sheridan et al. Lumbar Myeloid Cell Trafficking into Locomotor Networks after Thoracic Spinal Cord Injury Experimental Neurology 8/1/2016 [PMID: 27191729]
Ine Vandersmissen, Sander Craps, Maarten Depypere, Giulia Coppiello, Nick van Gastel, Frederik Maes et al. Endothelial Msx1 transduces hemodynamic changes into an arteriogenic remodeling response Journal of Cell Biology 9/28/2015 [PMID: 26391659]
Cedervall J, Zhang Y, Huang H, Zhang L, Femel J, Dimberg A, Olsson A Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals. Cancer Res, 2015-06-12;75(13):2653-62. 2015-06-12 [PMID: 26071254]
Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw T, Boyce J Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors. J Immunol, 2015-04-22;194(11):5061-8. 2015-04-22 [PMID: 25904552] (Western Blot, Mouse)	Western Blot	Mouse
Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice. PLoS ONE, 2015-03-23;10(3):e0119661. 2015-03-23 [PMID: 25799423] (IHC, Mouse)	IHC	Mouse
Gupta K, Donahue D, Sandoval-Cooper M, Castellino F, Ploplis V Abrogation of plasminogen activator inhibitor-1-vitronectin interaction ameliorates acute kidney injury in murine endotoxemia. PLoS ONE, 2015-03-23;10(3):e0120728. 2015-03-23 [PMID: 25799354] (Western Blot, Mouse)	Western Blot	Mouse
Maria J. Torres-Palsa, Matthew V. Koziol, Qingnian Goh, Peter A. Cicinelli, Jennifer M. Peterson, Francis X. Pizza Expression of intercellular adhesion molecule-1 by myofibers in mdx mice Muscle & Nerve 11/1/2015 [PMID: 25728314]
Caroline M. Sawicki, Daniel B. McKim, Eric S. Wohleb, Brant L. Jarrett, Brenda F. Reader, Diana M. Norden et al. Social defeat promotes a reactive endothelium in a brain region-dependent manner with increased expression of key adhesion molecules, selectins and chemokines associated with the recruitment of myeloid cells to the brain Neuroscience 8/27/2015 [PMID: 25445193]
Goh Q, Dearth C, Corbett J, Pierre P, Chadee D, Pizza F Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis. Exp Cell Res, 2014-09-30;331(2):292-308. 2014-09-30 [PMID: 25281303] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Ata Murat Kaynar, Sachin Yende, Lin Zhu, Daniel R Frederick, Robin Chambers, Christine L Burton et al. Effects of intra-abdominal sepsis on atherosclerosis in mice Critical Care 10/1/2014 [PMID: 25182529]
Norton , James E, Lytle , Andrew G, Shen , Shixue, Tzvetkov , Evgeni P, Dorfmeier , Corin L, McGettigan , James P ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo. PLoS ONE, 2014-01-29;9(1):e87098. 2014-01-29 [PMID: 24489846] (Western Blot, Hamster)	Western Blot	Hamster
Rampanelli , Elena, Dessing , Mark C, Claessen , Nike, Teske , Gwendoli, Joosten , Sander P, Pals , Steven T, Leemans , Jaklien, Florquin , Sandrine CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction. PLoS ONE, 2013-12-23;8(12):e84479. 2013-12-23 [PMID: 24376813] (IHC, Mouse)	IHC	Mouse
Bianca Hemmeryckx, Marc F. Hoylaerts, Eveline Deloose, Cor E. Van Hove, Paul Fransen, Hidde Bult et al. Age-associated pro-inflammatory adaptations of the mouse thoracic aorta Thrombosis and Haemostasis 1/10/2013 [PMID: 23925372]
Bianca Hemmeryckx, Marijke Gaekens, David J. Gallacher, Hua Rong Lu, Henri Roger Lijnen Effect of Rosiglitazone on Liver Structure and Function in Genetically Diabetic Akita Mice Basic & Clinical Pharmacology & Toxicology 7/1/2013 [PMID: 23789962]
Norifumi Ohashi, Tomohide Hori, Florence Chen, Sura Jermanus, Akimasa Nakao, Shinji Uemoto et al. Matrix metalloproteinase-9 in the initial injury after hepatectomy in mice World Journal of Gastroenterology 5/28/2013 [PMID: 23716982]
Mallavia B, Recio C, Oguiza A, Ortiz-Munoz G, Lazaro I, Lopez-Parra V, Lopez-Franco O, Schindler S, Depping R, Egido J, Gomez-Guerrero C Peptide inhibitor of NF-kappaB translocation ameliorates experimental atherosclerosis. Am J Pathol, 2013-04-16;182(5):1910-21. 2013-04-16 [PMID: 23597852] (IHC, Mouse)	IHC	Mouse
Dearth C, Goh Q, Marino J, Cicinelli P, Torres-Palsa M, Pierre P, Worth R, Pizza F Skeletal muscle cells express ICAM-1 after muscle overload and ICAM-1 contributes to the ensuing hypertrophic response. PLoS ONE, 2013-03-11;8(3):e58486. 2013-03-11 [PMID: 23505517] (ICC, IHC, Western Blot, Mouse)	ICC, IHC, Western Blot	Mouse
Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Biomark Insights, 2012-07-10;7(0):87-104. 2012-07-10 [PMID: 22837640] (Western Blot, Mouse)	Western Blot	Mouse
McMillan DH, Baglole CJ, Thatcher TH, Maggirwar S, Sime PJ, Phipps RP Lung-targeted overexpression of the NF-kappaB member RelB inhibits cigarette smoke-induced inflammation. Am. J. Pathol., 2011-05-05;179(1):125-33. 2011-05-05 [PMID: 21703398] (Western Blot, Mouse)	Western Blot	Mouse
Przemyslaw Sapieha, Andreas Stahl, Jing Chen, Molly R. Seaward, Keirnan L. Willett, Nathan M. Krah et al. 5-Lipoxygenase Metabolite 4-HDHA Is a Mediator of the Antiangiogenic Effect of omega -3 Polyunsaturated Fatty Acids Science Translational Medicine 2/9/2011 [PMID: 21307302]
Kato N, Yuzawa Y, Kosugi T The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76. 2009-05-14 [PMID: 19443639] (IHC-Fr, Western Blot, Mouse)	IHC-Fr, Western Blot	Mouse
Kubo M, Li TS, Kamota T, Ohshima M, Qin SL, Hamano K Increased expression of CXCR4 and integrin alphaM in hypoxia-preconditioned cells contributes to improved cell retention and angiogenic potency. J. Cell. Physiol., 2009-08-01;220(2):508-14. 2009-08-01 [PMID: 19415696] (Western Blot, Mouse)	Western Blot	Mouse
Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation. Am. J. Physiol. Heart Circ. Physiol., 2009-03-20;296(5):H1329-35. 2009-03-20 [PMID: 19304942] (IHC-P, Mouse)	IHC-P	Mouse
Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection. Eur. J. Immunol., 2009-01-01;39(1):85-95. 2009-01-01 [PMID: 19089809] (Western Blot, Mouse)	Western Blot	Mouse
Yi JH, Park SW, Brooks N, Lang BT, Vemuganti R PPARgamma agonist rosiglitazone is neuroprotective after traumatic brain injury via anti-inflammatory and anti-oxidative mechanisms. Brain Res., 2008-10-09;1244(0):164-72. 2008-10-09 [PMID: 18948087] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis. Kidney Int., 2008-09-17;75(1):52-9. 2008-09-17 [PMID: 18800031] (IHC-P, Mouse)	IHC-P	Mouse
Kim F, Pham M, Maloney E, Rizzo NO, Morton GJ, Wisse BE, Kirk EA, Chait A, Schwartz MW Vascular inflammation, insulin resistance, and reduced nitric oxide production precede the onset of peripheral insulin resistance. Arterioscler. Thromb. Vasc. Biol., 2008-09-04;28(11):1982-8. 2008-09-04 [PMID: 18772497] (Western Blot, Mouse)	Western Blot	Mouse
Casos K, Saiz MP, Ruiz-Sanz JI, Mitjavila MT Atherosclerosis prevention by a fish oil-rich diet in apoE(-/-) mice is associated with a reduction of endothelial adhesion molecules. Atherosclerosis, 2008-03-16;201(2):306-17. 2008-03-16 [PMID: 18439610] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice. Circulation, 2007-12-24;117(3):421-8. 2007-12-24 [PMID: 18158360] (IHC-P, Mouse)	IHC-P	Mouse
Yang XP, Mattagajasingh S, Su S, Chen G, Cai Z, Fox-Talbot K, Irani K, Becker LC Fractalkine upregulates intercellular adhesion molecule-1 in endothelial cells through CX3CR1 and the Jak Stat5 pathway. Circ. Res., 2007-09-20;101(10):1001-8. 2007-09-20 [PMID: 17885215] (Western Blot, Mouse)	Western Blot	Mouse
Rebholz B, Haase I, Eckelt B, Paxian S, Flaig MJ, Ghoreschi K, Nedospasov SA, Mailhammer R, Debey-Pascher S, Schultze JL, Weindl G, Forster I, Huss R, Stratis A, Ruzicka T, Rocken M, Pfeffer K, Schmid RM, Rupec RA Crosstalk between keratinocytes and adaptive immune cells in an IkappaBalpha protein-mediated inflammatory disease of the skin. Immunity, 2007-08-09;27(2):296-307. 2007-08-09 [PMID: 17692539] (IHC, Mouse)	IHC	Mouse
Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S High pressure promotes monocyte adhesion to the vascular wall. Circ. Res., 2007-03-29;100(8):1226-33. 2007-03-29 [PMID: 17395876] (IHC, Mouse)	IHC	Mouse
Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J. Exp. Med., 2006-11-20;203(12):2763-77. 2006-11-20 [PMID: 17116732] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Lim SY, Tennant GM, Kennedy S, Wainwright CL, Kane KA Activation of mouse protease-activated receptor-2 induces lymphocyte adhesion and generation of reactive oxygen species. Br. J. Pharmacol., 2006-09-18;149(5):591-9. 2006-09-18 [PMID: 16981001] (IHC-P, Mouse)	IHC-P	Mouse
Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am. J. Physiol. Lung Cell Mol. Physiol., 2005-12-22;290(5):L962-70. 2005-12-22 [PMID: 16373671] (IHC-P, Mouse)	IHC-P	Mouse
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Reviews for ICAM-1/CD54 Antibody (AF796) (6) 4.56

Average Rating: 4.5

(Based on 6 reviews)

We have 6 reviews tested in 2 species: Mouse, Other.

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Immunohistochemistry-Frozen ICAM-1/CD54 AF796

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reviewed by:
Chao Xiong

IHC-Fr

Mouse

03/14/2018

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Summary

Application	Immunohistochemistry-Frozen
Sample Tested	4% PFA-PBS fixed frozen sections from murine brain
Species	Mouse
Lot	GRG0215121

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reviewed by:
Verified Customer

IHC

Mouse

03/27/2017

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Summary

Application	Immunohistochemistry
Sample Tested	4T1 mouse breast cancer cell line,4T1 tumor section
Species	Mouse
Lot	GRG0215121

Comments

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). 4T1 tissues were cut 5 um. After deparaffin steps, the section was pre-treated using heat mediated antigen retrieval with Citrate buffer @ pH 6.0 (C9999, Thermo-Scientific) solution for 20 min at 100 C. Protein blocked was processed with superblock (#37515, Thermo Scientific) for 30 min. Afterwards, the section was then incubated with ICAM-1 pAb with 100X dilution of recommended concentration (100ug/500uL in PBS) for 1h at room temperature. A donkey anti-goat biotinylated secondary antibody (IgG H&L #ab7125, 1/200) was used to detect the primary and visualized using an HRP system (30 min incubation). DAB (#SK-4105, Vector) was used as the chromogen. The section was then counterstained with methyl green (#H-3402, Vector) and mounted with Cytoseal 60.

reviewed by:
Verified Customer

Mouse

01/09/2015

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Application	Immunofluorescence
Sample Tested	See PMID 20592225
Species	Mouse

reviewed by:
Verified Customer

Other

01/09/2015

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Summary

Application	Western Blot
Sample Tested	See PMID 22496445
Species	Other

reviewed by:
Verified Customer

Mouse

01/09/2015

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Summary

Application	Immunofluorescence
Sample Tested	See PMID 22661091
Species	Mouse

reviewed by:
Verified Customer

Mouse

01/09/2015

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Summary

Application	Immunofluorescence
Sample Tested	See PMID 23232501
Species	Mouse

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SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog #F0107).

Goat IgG Isotype Control [Unconjugated]
AB-108-C

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

TNF-alpha [Unconjugated]
210-TA-005

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4.5

5	3
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	Chao Xiong 03/14/2018
Application:	IHC-Fr
Species:	Mouse

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	Verified Customer 03/27/2017
Application:	IHC
Species:	Mouse

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	Verified Customer 01/09/2015
Application:	IF
Species:	Mouse

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Bioinformatics

Gene Symbol	ICAM1
Uniprot	Q3U8M7()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

ICAM-1/CD54 Antibody [Unconjugated]

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Publications for ICAM-1/CD54 Antibody (AF796)(88)

Reviews for ICAM-1/CD54 Antibody (AF796) (6) 4.56

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