Rat Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) Summary
Specificity |
This kit recognizes Rat OXA in samples. No significant cross-reactivity or interference between Rat OXA and analogues was observed. |
Standard Curve Range |
62.5-4000 pg/mL |
Sensitivity |
37.5 pg/mL |
Assay Type |
Competitive-ELISA |
Inter-Assay |
CV% < 5.39% |
Intra-Assay |
CV% < 4.44% |
Spike Recovery |
86-100% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
HCRT |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25X)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
|
Alternate Names for Rat Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)
Background
FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity (By similarity). SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum (By similarity). Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses (By similarity). SIMILARITY: Belongs to the orexin family.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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