Orexin A/Hypocretin-1 Antibody Summary
Immunogen |
Synthetic peptide, PLPDCCRQKTCSC, corresponding to amino acids 2-14 of human orexin A conjugated to KLH |
Epitope |
Peptide sequence: PLPDCCRQKTCSC |
Localization |
Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. |
Specificity |
NB100-65204 is specific for human orexin A. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Sheep |
Gene |
HCRT |
Purity |
Affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:200-1:800
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunohistochemistry-Paraffin 5-10 ug/ml
|
Reactivity Notes
Predicted cross-reactivities: Chimpanzee, Bovine, Equine, Dog, Rabbit, Porcine, Mouse, Rat, Rhesus Monkey
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.09% Sodium Azide |
Concentration |
5.0 mg/ml |
Purity |
Affinity purified |
Alternate Names for Orexin A/Hypocretin-1 Antibody
Background
Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B(hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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FAQs for Orexin A/Hypocretin-1 Antibody (NB100-65204) (0)
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