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Orexin A/Hypocretin-1 Antibody (97505) [Biotin]

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Orexin A/Hypocretin‑1 was detected in immersion fixed paraffin-embedded sections of Human Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Biotinylated Monoclonal Antibody (Catalog # BAM763) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Orexin A/Hypocretin‑1 was detected in immersion fixed paraffin-embedded sections of Human Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Biotinylated Monoclonal Antibody (Catalog # BAM763) ...read more
Orexin A/Hypocretin‑1 was detected in perfusion fixed frozen sections of Mouse Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Biotinylated Monoclonal Antibody (Catalog # BAM763) at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC
Clone
97505
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
MAB763-100
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB763-100
Carrier Free
MAB763-SP
Carrier Free
MAB763-500
Carrier Free
MAB763-100
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Orexin A/Hypocretin-1 Antibody (97505) [Biotin] Summary

Immunogen
KLH-coupled human Orexin A/Hypocretin‑1 synthetic peptide
Accession # O43612
Specificity
Detect human and mouse Orexin A/Hypocretin‑1.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HCRT
Purity Statement
Protein A or G purified from ascites
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-25 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Orexin A/Hypocretin-1 Antibody (97505) [Biotin]

  • HCRT
  • Hcrt1
  • hypocretin (orexin) neuropeptide precursor
  • Hypocretin1
  • Hypocretin-1
  • NRCLP1
  • Orexin A
  • orexin
  • OXHcrt
  • PPORX
  • PPOXHypocretin

Background

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein. Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism. The synthetic peptide immunogen corresponds to amino acid 34 - 66 of the human Orexin precursor (accession #O43612) (1).
  1. Sakurai, T. et al. (1998) Cell 92:573.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Orexin A/Hypocretin-1 Antibody (97505) [Biotin]
Species: Hu, Mu
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Bioinformatics

Gene Symbol HCRT
Uniprot