We have an inquiry of your product Cat. ID # NB400-148. Our customer would like to know the immunogen sequence of this product. If you cannot provide the detail, would you please check if this antibody covers amino acids 600-800?

Name: Niemann-Pick C1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB400-148
Rating: 4.2 (6 reviews)

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Accepted Answer

This antibody was made to a sequence found within amino acids 1230-1278.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Po, ChHa, Ha, PmSpecies Glossary
Applications	WB, ICC/IF, IHC, IP, MS, KD, KO
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free
Concentration	1.0 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB400-148AF350

Alexa Fluor 405 NB400-148AF405

Alexa Fluor 488 NB400-148AF488

Alexa Fluor 532 NB400-148AF532

Alexa Fluor 594 NB400-148AF594

Alexa Fluor 647 NB400-148AF647

Alexa Fluor 700 NB400-148AF700

Alexa Fluor 750 NB400-148AF750

Allophycocyanin NB400-148APC

Biotin NB400-148B

CoraFluor 1 NB400-148CL1

DyLight 350 NB400-148UV

DyLight 405 NB400-148V

DyLight 488 NB400-148G

DyLight 550 NB400-148R

DyLight 594 NB400-148DL594

DyLight 650 NB400-148C

DyLight 680 NB400-148FR

DyLight 755 NB400-148IR

FITC NB400-148F

HRP NB400-148H

Janelia Fluor 525 NB400-148JF525

Janelia Fluor 549 NB400-148JF549

Janelia Fluor 585 NB400-148JF585

Janelia Fluor 635 NB400-148JF635

Janelia Fluor 646 NB400-148JF646

Janelia Fluor 669 NB400-148JF669

PE NB400-148PE

PerCP NB400-148PCP

mFluor Violet 450 SE NB400-148MFV450

mFluor Violet 500 SE NB400-148MFV500

mFluor Violet 610 SE NB400-148MFV610

Catalog# & Formulation	Size	Price
BSA Free NB400-148SS

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB400-148AF350
Alexa Fluor 405	NB400-148AF405
Alexa Fluor 488	NB400-148AF488
Alexa Fluor 532	NB400-148AF532
Alexa Fluor 594	NB400-148AF594
Alexa Fluor 647	NB400-148AF647
Alexa Fluor 700	NB400-148AF700
Alexa Fluor 750	NB400-148AF750
Allophycocyanin	NB400-148APC
Biotin	NB400-148B
CoraFluor 1	NB400-148CL1
DyLight 350	NB400-148UV
DyLight 405	NB400-148V
DyLight 488	NB400-148G
DyLight 550	NB400-148R
DyLight 594	NB400-148DL594
DyLight 650	NB400-148C
DyLight 680	NB400-148FR
DyLight 755	NB400-148IR
FITC	NB400-148F
HRP	NB400-148H
Janelia Fluor 525	NB400-148JF525
Janelia Fluor 549	NB400-148JF549
Janelia Fluor 585	NB400-148JF585
Janelia Fluor 635	NB400-148JF635
Janelia Fluor 646	NB400-148JF646
Janelia Fluor 669	NB400-148JF669
PE	NB400-148PE
PerCP	NB400-148PCP
mFluor Violet 450 SE	NB400-148MFV450
mFluor Violet 500 SE	NB400-148MFV500
mFluor Violet 610 SE	NB400-148MFV610

Niemann-Pick C1 Antibody - BSA Free

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Publications for Niemann-Pick C1 Antibody (NB400-148)(54)

Reviews for Niemann-Pick C1 Antibody (NB400-148) (6) 4.26

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Immunogen	A synthetic peptide made to the C-terminal region of human Niemann-Pick C. [UniProt# O15118]
Localization	Late endosome and Lysosome membrane; Single-pass membrane protein.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	NPC1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.1% Sodium Azide
Concentration	1.0 mg/ml
Purity	Immunogen affinity purified

Publications using NB400-148	Applications	Species
Ji Eon Kim, So‐Young Park, Chulhwan Kwak, Yoonji Lee, Dae‐Geun Song, Jae Woo Jung, Haesong Lee, Eun‐Ae Shin, Yangie Pinanga, Kyung‐hee Pyo, Eun Hae Lee, Wonsik Kim, Soyeon Kim, Chang‐Duck Jun, Jeanho Yun, Sun Choi, Hyun‐Woo Rhee, Kwang‐Hyeon Liu, Jung Weon Lee Glucose‐mediated mitochondrial reprogramming by cholesterol export at TM4SF5‐enriched mitochondria‐lysosome contact sites Cancer Communications 2023-12-22 [PMID: 38133457]
N?sková H, Cortizo FG, Schwenker LS et al. Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome The Journal of biological chemistry 2023-07-24 [PMID: 37495107] (WB, Human) Details: 1:1000 WB dilution	WB	Human
Skop�l A, Ujlaki G, Gerencs�r AT et al. Adenosine A(2A) Receptor Activation Regulates Niemann-Pick C1 Expression and Localization in Macrophages Current Issues in Molecular Biology 2023-06-07 [PMID: 37367064] (WB)	WB
Martella N, Colardo M, Sergio W et al. Lavender Essential Oil Modulates Hepatic Cholesterol Metabolism in HepG2 Cells Current issues in molecular biology 2023-01-03 [PMID: 36661512] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Weng Y, Shepherd D, Liu Y et al. Inhibition of the Niemann-Pick C1 protein is a conserved feature of multiple strains of pathogenic mycobacteria Nature Communications 2022-09-09 [PMID: 36085278]
Colardo M, Petraroia M, Lerza L et al. NGF Modulates Cholesterol Metabolism and Stimulates ApoE Secretion in Glial Cells Conferring Neuroprotection against Oxidative Stress International Journal of Molecular Sciences 2022-04-27 [PMID: 35563230] (WB)	WB
Cheng YS, Zhang T, Ma X et al. A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes Nature Chemical Biology 2021-12-01 [PMID: 34799735]
Goedeke L, Canfran-Duque A, Rotllan N Et al. MMAB promotes negative feedback control of cholesterol homeostasis Nature communications 2021-11-08 [PMID: 34750386] (WB, Human)	WB	Human
Walch P, Selkrig J, Knodler La Et Al. Global mapping of Salmonella enterica-host protein-protein interactions during infection Cell host & microbe 2021-07-02 [PMID: 34237247]
Guix FX, Capit�n AM, Casadom�-Perales � et al. Increased exosome secretion in neurons aging in vitro by NPC1-mediated endosomal cholesterol buildup Life Science Alliance 2021-08-01 [PMID: 34183444]
Kim TY, Jeon S, Jang Y et al. Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion Experimental & molecular medicine 2021-05-25 [PMID: 34035463] (WB, Human)	WB	Human
Piggott V, Lloyd S, Matchynski J et al. Traumatic Stress, Chronic Ethanol Exposure, or the Combination, Alter Cannabinoid System Components in Reward and Limbic Regions of the Mouse Brain Molecules 2021-04-30 [PMID: 33917316]
Jiang D, Lee H, Pardridge WM Plasmid DNA gene therapy of the Niemann-Pick C1 mouse with transferrin receptor-targeted Trojan horse liposomes Sci Rep 2020-08-07 [PMID: 32770132] (WB, Mouse)	WB	Mouse
Colaco A, FernAndez-SuArez ME, Shepherd D et al. Unbiased yeast screens identify cellular pathways affected in Niemann-Pick disease type C Life Sci Alliance 2020-07-01 [PMID: 32487688]
Eriksson I, WAster P, Ollinger K Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins Cell Death Dis 2020-05-14 [PMID: 32409651] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Brogden G, Shammas H, Walters F et Al. Different Trafficking Phenotypes of Niemann-Pick C1 Gene Mutations Correlate with Various Alterations in Lipid Storage, Membrane Composition and Miglustat Amenability Int J Mol Sci 2020-03-19 [PMID: 32204338] (IB, Human)	IB	Human
Dardis A, Zampieri S, Gellera C et Al. Molecular Genetics of Niemann-Pick Type C Disease in Italy: An Update on 105 Patients and Description of 18 NPC1 Novel Variants J Clin Med 2020-03-03 [PMID: 32138288] (WB, Human)	WB	Human
Tonini C, Colardo M, et al. Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins by JQ1 Unravels a Novel Epigenetic Modulation to Control Lipid Homeostasis. Int J Mol Sci 2020-02-14 [PMID: 32075110] (WB, Human)	WB	Human
Mitroi DN, Pereyra-GOmez G, Soto-Huelin B et al. NPC1 enables cholesterol mobilization during long-term potentiation that can be restored in Niemann-Pick disease type C by CYP46A1 activation EMBO Rep. 2019-09-18 [PMID: 31535451] (ICC/IF, WB, Mouse)	ICC/IF, WB	Mouse
Heybrock S, Kanerva K, Meng Y et al. Lysosomal integral membrane protein-2 (LIMP-2/SCARB2) is involved in lysosomal cholesterol export Nat Commun 2019-08-06 [PMID: 31387993] (KD, WB, Mouse, Human)	KD, WB	Mouse, Human
Vacca F, Vossio S et al. Cyclodextrin triggers MCOLN1-dependent endo-lysosome secretion in Niemann-Pick type C cells. J Lipid Res 2019-01-04 [PMID: 30709900] (WB, Human)	WB	Human
Ebrahimi-Fakhari D, Wahlster L, Bartz F et al. Reduction of TMEM97 increases NPC1 protein levels and restores cholesterol trafficking in Niemann-pick type C1 disease cells Hum. Mol. Genet. 2016-07-04 [PMID: 27378690] (ICC/IF)	ICC/IF
Ferrante A, De Nuccio C, Pepponi R et al. Stimulation of adenosine A2A receptors reduces intracellular cholesterol accumulation and rescues mitochondrial abnormalities in human neural cell models of Niemann-Pick C1. Neuropharmacology. 2015-11-26 [PMID: 26631535] (WB, Human)	WB	Human
Li J, Deffieu MS, Lee PL et al. Glycosylation inhibition reduces cholesterol accumulation in NPC1 protein-deficient cells. Proc. Natl. Acad. Sci. U.S.A. 2015-11-17 [PMID: 26578804] (WB, Chinese Hamster)	WB	Chinese Hamster
Kanerva K, Uronen RL, Blom T et al. LDL Cholesterol Recycles to the Plasma Membrane via a Rab8a-Myosin5b-Actin-Dependent Membrane Transport Route. Dev Cell. 2013-11-11 [PMID: 24209575] (IP, Human)	IP	Human
Armstrong A, Mattsson N, Appelqvist H et al. Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer's Disease. Neuromolecular Med. 2013-10-08 [PMID: 24101586] (WB, Human)	WB	Human
Cianciola NL, Greene DJ, Morton RE, Carlin CR. Adenovirus RIDalpha uncovers a novel pathway requiring ORP1L for lipid droplet formation independent of NPC1. Mol Biol Cell. 2013-11-01 [PMID: 24025716] (ICC/IF, Human)	ICC/IF	Human
Roszell BR, Tao JQ, Yu KJ et al. Pulmonary Abnormalities in Animal Models Due to Niemann-Pick Type C1 (NPC1) or C2 (NPC2) Disease. PLoS One 2013-01-01 [PMID: 23843985] (WB, Mouse)	WB	Mouse
Fu R, Wassif CA, Yanjanin NM et al. Efficacy of N-acetylcysteine in phenotypic suppression of Mouse models of Niemann-Pick disease, type C1. Hum Mol Genet 2013-05-21 [PMID: 23666527] (WB, Mouse)	WB	Mouse
Goedeke L, Vales-Lara FM, Fenstermaker M et al. A Regulatory Role for MicroRNA 33(Asterisk) in Controlling Lipid Metabolism Gene Expression. Mol Cell Biol 2013-06-01 [PMID: 23547260] (WB, Human)	WB	Human
Ohgane K, Karaki F, Dodo K, Hashimoto Y. Discovery of Oxysterol-Derived Pharmacological Chaperones for NPC1: Implication for the Existence of Second Sterol-Binding Site Chem Biol 2013-03-21 [PMID: 23521797] (WB, Human)	WB	Human
Zampieri S, Bembi B, Rosso N et al. Treatment of Human Fibroblasts Carrying NPC1 Missense Mutations with MG132 Leads to an Improvement of Intracellular Cholesterol Trafficking. JIMD Rep. 2012-01-01 [PMID: 23430855] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Du X, Kazim AS, Brown AJ, Yang H. An essential role of Hrs/Vps27 in endosomal cholesterol trafficking.. Cell Rep. 2012-01-26 [PMID: 22832105] (WB, Human) Details: SREBP2 antibody (clone 1D2) was used for WB analysis of lysates from HeLa cells that were transfected with siNPC1, siHrs, or a universal control siCtrl, and were then stimulated with 50ug/ml of LDL (Figure 3C).	WB	Human
Blom T, Li Z, Bittman R, Somerharju P, Ikonen E. Tracking Sphingosine Metabolism and Transport in Sphingolipidoses: NPC1 Deficiency as a Test Case Traffic 2012-05-18 [PMID: 22607065] (WB, Human)	WB	Human
Coleman EM, Walker TN, Hildreth JEK. Loss of Niemann Pick type C proteins 1 and 2 greatly enhances HIV infectivity and is associated with accumulation of HIV Gag and cholesterol in late endosomes/lysosomes. Virology Journal 249(1):3. 2012-01-01 [PMID: 22273177]
Xiong, Q Rikihisa, Y. Subversion of NPC1 pathway of cholesterol transport by Anaplasma phagocytophilum Cellular Microbiology 2012-01-01 [PMID: 22212234] (ICC/IF)	ICC/IF
Shichiri M, Kono N, Shimanaka Y et al. A novel role for alpha-tocopherol transfer protein (alpha-TTP) in protecting against chloroquine toxicity J Biol Chem 2012-01-20 [PMID: 22147702] (WB, Rat)	WB	Rat
Piccoli E, Nadai M, Caretta CM et al. Amiodarone impairs trafficking through late endosomes inducing a Niemann-Pick C-like phenotype. Biochemical Pharmacology. 2011-08-23 [PMID: 21878321] (ICC/IF, Hamster)	ICC/IF	Hamster
Hsieh CH, Chang HT, Shen WC, Shyu WC, Liu RS. Imaging the Impact of Nox4 in Cycling Hypoxia-mediated U87 Glioblastoma Invasion and Infiltration. Mol Imaging Biol. 2011-08-26 [PMID: 21870211] (IF/IHC, Mouse)	IF/IHC	Mouse
Lopez ME, Klein AD, Dimbil UJ, Scott MP. Anatomically defined neuron-based rescue of neurodegenerative niemann-pick type C disorder. J. Neurosci. 31(12):4367-4378. 2011-03-23 [PMID: 21430138]
Cima I, Schiess R, Wild P et al. Cancer genetics-guided discovery of serum biomarker signatures for diagnosis and prognosis of prostate cancer. Proc Natl Acad Sci U S A 2011-02-22 [PMID: 21300890]
Skov M, Tonnesen CK, Hansen GH et al. Dietary cholesterol induces trafficking of intestinal Niemann-Pick Type C1 Like 1 from the brush border to endosomes. Am J Physiol Gastrointest Liver Physiol 2011-01-01 [PMID: 21051527] (IHC-P, EM, Porcine)	IHC-P, EM	Porcine
Blom T, Back N, Mutka AL et al. FTY720 stimulates 27-hydroxycholesterol production and confers atheroprotective effects in human primary macrophages. Circ Res;106(4):720-9. 2010-03-05 [PMID: 20056921] (WB, Human, Chinese Hamster)	WB	Human, Chinese Hamster
Charman M, Kennedy BE, Osborne N et al. MLN64 mediates egress of cholesterol from endosomes to mitochondria in the absence of functional Niemann-Pick Type C1 protein. J Lipid Res;51(5):1023-34. 2010-05-01 [PMID: 19965586] (WB, Mouse)	WB	Mouse
Abi-Mosleh L, Infante RE, Radhakrishnan A et al. Cyclodextrin overcomes deficient lysosome-to-endoplasmic reticulum transport of cholesterol in Niemann-Pick type C cells. Proc Natl Acad Sci U S A;106(46):19316-21. 2009-11-17 [PMID: 19884502]
Bartz F, Kern L, Erz D et al. Identification of cholesterol-regulating genes by targeted RNAi screening. Cell Metab. 2009-07-01 [PMID: 19583955] (ICC/IF, IP, WB, Human)	ICC/IF, IP, WB	Human
Rocha N, Kuijl C, van der Kant R et al. Cholesterol sensor ORP1L contacts the ER protein VAP to control Rab7-RILP-p150Glued late endosome positioning. J Cell Biol;185(7):1209-1225. 2009-01-01 [PMID: 19564404]
Podechard N, Le Ferrec E, Rebillard A et al. NPC1 repression contributes to lipid accumulation in human macrophages exposed to environmental aryl hydrocarbons. Cardiovasc Res;82(2):361-370. 2009-01-01 [PMID: 19131362] (WB, Human, Mouse)	WB	Human, Mouse
Dudley BA Role of ceramide-1 phosphate in regulation of sphingolipid and eicosanoid metabolism in lung epithelial cells Thesis 2020-01-01
Burke KT, Colvin PL, Myatt L et al. Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster. J Lipid Res;50(6):1146-1155. 2009-01-01 [PMID: 19122238] (WB, Chinese Hamster)	WB	Chinese Hamster
Gelsthorpe ME et al. Niemann-Pick type C1 I1061T mutant encodes a functional protein that is selected for endoplasmic reticulum-associated degradation due to protein misfolding. J Biol Chem;283(13):8229-36. 2008-03-28 [PMID: 18216017] (WB, IP, ICC/IF, Human)	WB, IP, ICC/IF	Human
Infante, R E et al. Purified NPC1 protein. I. Binding of cholesterol and oxysterols to a 1278-amino acid membrane protein. J Biol Chem. 283(2):1052-63. Epub 2007 Nov 6. 2008-01-11 [PMID: 17989073]
Frolov A et al. Cholesterol overload promotes morphogenesis of a Niemann-Pick C (NPC)-like compartment independent of inhibition of NPC1 or HE1/NPC2 function. J Biol Chem;276(49):46414-21. 2001-12-07 [PMID: 11571306] (ICC/IF, Human)	ICC/IF	Human
Millard E et al. Niemann-pick type C1 (NPC1) overexpression alters cellular cholesterol homeostasis. J Biol Chem;275(49):38445-51. 2000-12-08 [PMID: 10964915] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Show All 54 Publications. Collapse Publications.

Gene Symbol	NPC1
Entrez	4864(Human) 18145(Mouse)
Uniprot	O15118(Human)

	Verified Customer 10/23/2019
Application:	IF
Species:	Human

	Verified Customer 11/27/2018
Application:	ICC
Species:	Human

	Verified Customer 11/01/2018
Application:	WB
Species:	Human