Home » Niemann-Pick C1 » Niemann-Pick C1 Antibodies » Niemann-Pick C1 Antibody [Alexa Fluor® 350]
Novus Biologicals products are now on bio-techne.com

Niemann-Pick C1 Antibody [Alexa Fluor® 350]

 Submit a Review

Images

 
There are currently no images for Niemann-Pick C1 Antibody (NB400-148AF350).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ChHa, Ha, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IP, MS, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB400-148AF405
Alexa Fluor 488
NB400-148AF488
Alexa Fluor 532
NB400-148AF532
Alexa Fluor 594
NB400-148AF594
Alexa Fluor 647
NB400-148AF647
Alexa Fluor 700
NB400-148AF700
Alexa Fluor 750
NB400-148AF750
Allophycocyanin
NB400-148APC
Biotin
NB400-148B
CoraFluor 1
NB400-148CL1
DyLight 350
NB400-148UV
DyLight 405
NB400-148V
DyLight 488
NB400-148G
DyLight 550
NB400-148R
DyLight 594
NB400-148DL594
DyLight 650
NB400-148C
DyLight 680
NB400-148FR
DyLight 755
NB400-148IR
FITC
NB400-148F
HRP
NB400-148H
Janelia Fluor 525
NB400-148JF525
Janelia Fluor 549
NB400-148JF549
Janelia Fluor 585
NB400-148JF585
Janelia Fluor 635
NB400-148JF635
Janelia Fluor 646
NB400-148JF646
Janelia Fluor 669
NB400-148JF669
PE
NB400-148PE
PerCP
NB400-148PCP
Unconjugated
NB400-148
mFluor Violet 450 SE
NB400-148MFV450
mFluor Violet 500 SE
NB400-148MFV500
mFluor Violet 610 SE
NB400-148MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB400-148
BSA Free
NB400-148SS
BSA Free
NB400-148
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Niemann-Pick C1 Antibody [Alexa Fluor® 350] Summary

Immunogen
A synthetic peptide made to the C-terminal region of human Niemann-Pick C. [UniProt# O15118]
Localization
Late endosome and Lysosome membrane; Single-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Electron Microscopy
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human, mouse, rat and Chinese hamster and primate (PMID 22212234). Porcine reactivity reported in scientific literature (PMID: 21051527) Results with mouse in Western blot have been mixed. Use in Hamster reported in scientific literature (PMID:21878321).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Niemann-Pick C1 Antibody [Alexa Fluor® 350]

  • FLJ98532
  • Niemann-Pick C1 protein
  • Niemann-Pick disease, type C1
  • Niemann-Pick Type C1
  • NPC
  • NPC1
  • SLC65A1

Background

Niemann-Pick type C1 (NPC1) is a member of a family of genes encoding membrane-bound proteins containing putative sterol sensing domains. The protein regulates cholesterol transport from late endosomes-lysosomes to other intracellular compartments. NPC1 overexpression increases both the rate of trafficking of low density lipoprotein cholesterol to the endoplasmic reticulum and the rate of delivery of endosomal cholesterol to the plasma membrane. NPC disease is an inherited neurovisceral lipid storage disorder of unesterified cholesterol accumulation in lysosomes. It is characterized by progressive neural and liver degeneration, resulting from inactivating mutations in NPC1, in most cases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

hnRNP C1 + C2
Antibody (CL2593)
NBP2-36776
Western Blot hnRNP C1 + C2 Antibody (CL2593)
Immunohistochemistry hnRNP C1 + C2 Antibody (CL2593)
Immunohistochemistry hnRNP C1 + C2 Antibody (CL2593)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Niemann-Pick type
C2 Antibody
NBP1-84012
Immunohistochemistry-Paraffin Niemann-Pick type C2 Antibody
Immunohistochemistry-Paraffin Niemann-Pick type C2 Antibody
Immunohistochemistry-Paraffin Niemann-Pick type C2 Antibody
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
ARHGAP4 Antibody
NBP1-88527
Western Blot ARHGAP4 Antibody
Immunohistochemistry-Paraffin ARHGAP4 Antibody
Immunohistochemistry-Paraffin ARHGAP4 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
PFDN4 Antibody -
Azide and BSA Free
NBP2-93935
Immunocytochemistry/ Immunofluorescence PFDN4 Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence PFDN4 Antibody - Azide and BSA Free
Western Blot PFDN4 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
AGFG2 Antibody
NBP1-83213
Immunohistochemistry-Paraffin AGFG2 Antibody
Immunohistochemistry-Paraffin AGFG2 Antibody
Immunohistochemistry-Paraffin AGFG2 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
PDLIM7 Antibody
NBP1-84841
Immunohistochemistry-Paraffin PDLIM7 Antibody
Immunocytochemistry/ Immunofluorescence PDLIM7 Antibody
Western Blot PDLIM7 Antibody
(3 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Ran Antibody
(8D1A6) - BSA Free
NBP2-61832
ELISA Ran Antibody (8D1A6) - BSA Free
Flow Cytometry Ran Antibody (8D1A6) - BSA Free
Immunocytochemistry/ Immunofluorescence Ran Antibody (8D1A6) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Neutrophil
Elastase/ELA2 [Biotin
DY4517-05
N/A Neutrophil Elastase/ELA2 [Biotin]
(14 Publications)
Species: Mu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(809 Publications)
Species: Hu
Applications: BA
RanBP2 Antibody
NB120-2938
Western Blot RanBP2 Antibody
Immunohistochemistry RanBP2 Antibody
Western Blot RanBP2 Antibody
(2 Publications)
Species: Bv, Hu, Mu
Applications: IHC, IHC-P, WB
Nup153 Antibody
NB100-93329
Immunocytochemistry/ Immunofluorescence Nup153 Antibody
Western Blot Nup153 Antibody
Western Blot Nup153 Antibody
(2 Reviews)
(4 Publications)
Species: Hu
Applications: ICC/IF, IP, KO, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Nestin Antibody
(196908) [Unconjugate
MAB1259
Immunocytochemistry Nestin Antibody (196908) [Unconjugated]
Intracellular Staining by Flow Cytometry Nestin Antibody (196908) [Unconjugated]
Western Blot Nestin Antibody (196908) [Unconjugated]
(3 Reviews)
(159 Publications)
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
Tau Antibody (2E9)
NBP2-25162
Immunocytochemistry/ Immunofluorescence Tau Antibody (2E9)
Western Blot Tau Antibody (2E9)
Immunocytochemistry/ Immunofluorescence Tau Antibody (2E9)
(5 Publications)
Species: Bv, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, PLA, WB
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
ABCA1 Antibody -
BSA Free
NB400-105
Dual RNAscope ISH-IHC ABCA1 Antibody - BSA Free
Immunohistochemistry-Paraffin ABCA1 Antibody - BSA Free
Western Blot ABCA1 Antibody - BSA Free
(7 Reviews)
(408 Publications)
Species: Ca, Ch, ChHa, Eq, Ha, Hu, Mu, Md, Po, Pm, Rb, Rt
Applications: B/N, ChIP, ChIP, Dual ISH-IHC, ELISA, Flow, GS, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PCR, Simple Western, WB
BDNF [HRP]
DBD00
N/A BDNF [HRP]
N/A BDNF [HRP]
N/A BDNF [HRP]
(104 Publications)
Species: Hu
Applications: ELISA
beta Amyloid
Antibody (MOAB-2) - B ...
NBP2-13075
Immunohistochemistry-Frozen beta Amyloid Antibody (MOAB-2) - BSA Free
Immunohistochemistry beta Amyloid Antibody (MOAB-2) - BSA Free
Immunocytochemistry/ Immunofluorescence beta Amyloid Antibody (MOAB-2) - BSA Free
(2 Reviews)
(70 Publications)
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(643 Publications)
Species: Mu
Applications: ELISA
Niemann-Pick C1
Antibody [Alexa Fluor ...
NB400-148AF350
Niemann-Pick C1 Antibody [Alexa Fluor® 350]
Species: Hu, Mu, Rt, Po, ChHa, Ha, Pm
Applications: WB, ICC/IF, IHC, IP, MS, KD, KO

Publications for Niemann-Pick C1 Antibody (NB400-148AF350) (0)

There are no publications for Niemann-Pick C1 Antibody (NB400-148AF350).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Niemann-Pick C1 Antibody (NB400-148AF350) (0)

There are no reviews for Niemann-Pick C1 Antibody (NB400-148AF350). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Niemann-Pick C1 Antibody (NB400-148AF350). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
    • Niemann-Pick C1 Antibody (<a href="http://www.novusbio.com/NB400-148" target="_self">NB400-148</a>) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # <a href="http://www.novusbio.com/H00010577-D01P" target="_self">H00010577-D01P</a>, but this antibody is yet to be established for IHC application. However, if you would like to try this antibody for IHC-P application you would qualify for our Innovators Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product. Read more about <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a>.

Secondary Antibodies

 

Isotype Controls

Additional Niemann-Pick C1 Products

Research Areas for Niemann-Pick C1 Antibody (NB400-148AF350)

Find related products by research area.

Blogs on Niemann-Pick C1.

Check out the latest blog posts on Niemann-Pick C1.
NPC1: A Potential Target For Triple-Negative Breast Cancer
By Natalia Gurule, PhD Breast Cancer is a Heterogeneous DiseaseBreast cancer is the most frequently identified malignancy in women, accounting for 30% of diagnosed cases of cancer in women in the US annuall...  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Niemann-Pick C1 Antibody [Alexa Fluor&#174; 350] and receive a gift card or discount.


Bioinformatics

Gene Symbol NPC1