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Niemann-Pick C1 Antibody [DyLight 680]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ChHa, Ha, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IP, MS, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 680

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB400-148AF350
Alexa Fluor 405
NB400-148AF405
Alexa Fluor 488
NB400-148AF488
Alexa Fluor 532
NB400-148AF532
Alexa Fluor 594
NB400-148AF594
Alexa Fluor 647
NB400-148AF647
Alexa Fluor 700
NB400-148AF700
Alexa Fluor 750
NB400-148AF750
Allophycocyanin
NB400-148APC
Biotin
NB400-148B
CoraFluor 1
NB400-148CL1
DyLight 350
NB400-148UV
DyLight 405
NB400-148V
DyLight 488
NB400-148G
DyLight 550
NB400-148R
DyLight 594
NB400-148DL594
DyLight 650
NB400-148C
DyLight 755
NB400-148IR
FITC
NB400-148F
HRP
NB400-148H
Janelia Fluor 525
NB400-148JF525
Janelia Fluor 549
NB400-148JF549
Janelia Fluor 585
NB400-148JF585
Janelia Fluor 635
NB400-148JF635
Janelia Fluor 646
NB400-148JF646
Janelia Fluor 669
NB400-148JF669
PE
NB400-148PE
PerCP
NB400-148PCP
Unconjugated
NB400-148
mFluor Violet 450 SE
NB400-148MFV450
mFluor Violet 500 SE
NB400-148MFV500
mFluor Violet 610 SE
NB400-148MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB400-148
BSA Free
NB400-148SS
BSA Free
NB400-148
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Niemann-Pick C1 Antibody [DyLight 680] Summary

Immunogen
A synthetic peptide made to the C-terminal region of human Niemann-Pick C. [UniProt# O15118]
Localization
Late endosome and Lysosome membrane; Single-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Electron Microscopy
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human, mouse, rat and Chinese hamster and primate (PMID 22212234). Porcine reactivity reported in scientific literature (PMID: 21051527) Results with mouse in Western blot have been mixed. Use in Hamster reported in scientific literature (PMID:21878321).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Niemann-Pick C1 Antibody [DyLight 680]

  • FLJ98532
  • Niemann-Pick C1 protein
  • Niemann-Pick disease, type C1
  • Niemann-Pick Type C1
  • NPC
  • NPC1
  • SLC65A1

Background

Niemann-Pick type C1 (NPC1) is a member of a family of genes encoding membrane-bound proteins containing putative sterol sensing domains. The protein regulates cholesterol transport from late endosomes-lysosomes to other intracellular compartments. NPC1 overexpression increases both the rate of trafficking of low density lipoprotein cholesterol to the endoplasmic reticulum and the rate of delivery of endosomal cholesterol to the plasma membrane. NPC disease is an inherited neurovisceral lipid storage disorder of unesterified cholesterol accumulation in lysosomes. It is characterized by progressive neural and liver degeneration, resulting from inactivating mutations in NPC1, in most cases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Niemann-Pick C1
Antibody [DyLight 680
NB400-148FR
Niemann-Pick C1 Antibody [DyLight 680]
Species: Hu, Mu, Rt, Po, ChHa, Ha, Pm
Applications: WB, ICC/IF, IHC, IP, MS, KD, KO

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Niemann-Pick C1 Antibody (NB400-148FR). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?
    • Niemann-Pick C1 Antibody (<a href="http://www.novusbio.com/NB400-148" target="_self">NB400-148</a>) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # <a href="http://www.novusbio.com/H00010577-D01P" target="_self">H00010577-D01P</a>, but this antibody is yet to be established for IHC application. However, if you would like to try this antibody for IHC-P application you would qualify for our Innovators Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product. Read more about <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a>.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol NPC1