![Western Blot: MLYCD Antibody [NBP1-32797] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MLYCD-Antibody-Western-Blot-NBP1-32797-img0019.jpg "Western Blot: MLYCD Antibody [NBP1-32797] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MLYCD. The exact sequence is proprietary. |
| Localization | Mitochondrion, Cytoplasm, Peroxisome |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MLYCD |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western internal validation: Purified mitochondrial and cytosol preparations of human SVG-A cells as sample; separated by size; matrix was 12-230 kDa; detected by Chemiluminescence. |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
Simple Western | Human | 08/03/2016 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 08/03/2016 |
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| Application: | Simple Western | |
| Species: | Human |
| Gene Symbol | MLYCD |
![Immunocytochemistry/Immunofluorescence: MLYCD Antibody [NBP1-32797] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCD stained by MCD antibody [N2C1], Internal diluted at 1:2000. Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/MLYCD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32797-img0015.jpg "Immunocytochemistry/Immunofluorescence: MLYCD Antibody [NBP1-32797] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCD stained by MCD antibody [N2C1], Internal diluted at 1:2000. Blue: Hoechst 33342 staining.Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: MLYCD Antibody [NBP1-32797] - Mouse muscle. MCD antibody [N2C1], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MLYCD-Antibody-Immunohistochemistry-Paraffin-NBP1-32797-img0009.jpg "Immunohistochemistry-Paraffin: MLYCD Antibody [NBP1-32797] - Mouse muscle. MCD antibody [N2C1], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: MLYCD Antibody [NBP1-32797] - A. 30 ug C8D30 whole cell lysate/extract 7.5% SDS-PAGE MCD antibody dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MLYCD-Antibody-Western-Blot-NBP1-32797-img0013.jpg "Western Blot: MLYCD Antibody [NBP1-32797] - A. 30 ug C8D30 whole cell lysate/extract 7.5% SDS-PAGE MCD antibody dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: MLYCD Antibody [NBP1-32797] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MLYCD-Antibody-Western-Blot-NBP1-32797-img0017.jpg "Western Blot: MLYCD Antibody [NBP1-32797] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: MLYCD Antibody [NBP1-32797] - H661 xenograft, using MCD antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MLYCD-Antibody-Immunohistochemistry-Paraffin-NBP1-32797-img0007.jpg "Immunohistochemistry-Paraffin: MLYCD Antibody [NBP1-32797] - H661 xenograft, using MCD antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: MLYCD Antibody [NBP1-32797] - Mouse kidney. MCD antibody [N2C1], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MLYCD-Antibody-Immunohistochemistry-Paraffin-NBP1-32797-img0008.jpg "Immunohistochemistry-Paraffin: MLYCD Antibody [NBP1-32797] - Mouse kidney. MCD antibody [N2C1], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
