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AMPK beta 1 Antibody [Unconjugated]

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human AMPK beta 1 and AMPK beta 2 (2 ng/lane) were included. A specific band for AMPK beta 1 was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal ...read more
AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 3 µg/mL ...read more
AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of Mouse Kidney using Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 3 µg/mL ...read more
AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of Rat Kidney using Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 5 µg/mL for ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
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AF2854-SP
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AMPK beta 1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human AMPK beta 1
Met1-Ile270
Accession # Q9Y478
Specificity
Detects human, mouse, and rat AMPK beta 1 in Western blots. In Western blots, no cross-reactivity with recombinant human AMPK beta 2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PRKAB1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using AF2854.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMPK beta 1 Antibody [Unconjugated]

  • 5'-AMP-activated protein kinase beta-1 subunit
  • AMP-activated protein kinase beta subunit
  • AMPK beta -1 chain
  • AMPK beta 1
  • AMPK beta 1,5'-AMP-activated protein kinase subunit beta-1
  • AMPK subunit beta-1
  • AMPK
  • AMPKb
  • HAMPKb
  • MGC17785
  • PRKAB1
  • protein kinase, AMP-activated, beta 1 non-catalytic subunit
  • protein kinase, AMP-activated, noncatalytic, beta-1

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The beta 1 subunit is expressed at higher levels than the beta 2 subunit in liver, while beta 2 is more abundant than beta 1 in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase complex as a therapeutic target in heart disease, obesity, and diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AMPK beta 1 Antibody (AF2854)(2)


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Bioinformatics

Gene Symbol PRKAB1
Uniprot