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IRF1 Antibody [Unconjugated]

 (2 Publications)
 (2 Reviews)
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Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse IRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4715) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for IRF1 was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was ...read more
Whole cell lysates from MCF7 and SUM159 cells were loaded with 50 ug/lane.10% SDS-PAGE. IRF1 Antibody (AF4715) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4715-SP
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IRF1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IRF1
Thr147-Pro329
Accession # P15314
Specificity
Detects mouse IRF1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IRF1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF4715 in the following application:
Publications
Read Publications using
AF4715 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRF1 Antibody [Unconjugated]

  • interferon regulatory factor 1
  • IRF1
  • MAR
  • MARIRF-1

Background

IRF1 is a member of the interferon regulator transcription factor family, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF1 knock out mice show defects in NK and T cell function, as well as, develop CD30+ lymphoproliferative disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRF1 Antibody (AF4715)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC.


Filter By Application
ICC
(1)
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Filter By Species
Mouse
(1)
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Reviews for IRF1 Antibody (AF4715) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using AF4715:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Yongkang Yang
 WB Human 11/28/2024
View

Summary

ApplicationWestern Blot
Sample Testedbreast cancer MCF7 cells,SUM-159PT
SpeciesHuman
LotCALP0120091

Comments

Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***
  4
reviewed by:
Verified Customer
 WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22464096
SpeciesMouse

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IRF1 Antibody (AF4715) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Goat IgG Isotype Control [Unconjugated]
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1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
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Recent Reviews

4.5
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Western Blot IRF1 AF4715
Enlarge
 Yongkang Yang
11/28/2024
Application: WB
Species: Human
read this review
 
 Verified Customer
01/08/2015
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol IRF1
Uniprot