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IRF1 Antibody

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 (1 Review)
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Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse IRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4715) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for IRF1 was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF4715-SP
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IRF1 Antibody Summary

Immunogen
E. coli-derived recombinant mouse IRF1
Thr147-Pro329
Accession # P15314
Specificity
Detects mouse IRF1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IRF1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF4715 in the following application:
Publications
Read Publications using
AF4715 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRF1 Antibody

  • interferon regulatory factor 1
  • IRF1
  • MAR
  • MARIRF-1

Background

IRF1 is a member of the interferon regulator transcription factor family, a family characterized by a helix-turn-helix DNA binding domain enriched in tryptophan repeats. IRF family members show diverse cellular regulation of interferon-stimulated gene transcription, viral-mediated gene activation, apoptosis, differentiation, and cellular growth. IRF1 knock out mice show defects in NK and T cell function, as well as, develop CD30+ lymphoproliferative disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRF1 Antibody (AF4715)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC.


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ICC
(1)
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Mouse
(1)
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Review for IRF1 Antibody (AF4715) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF4715:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 WB Mouse 01/08/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22464096
SpeciesMouse

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FAQs for IRF1 Antibody (AF4715) (0)

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Secondary Antibodies

 

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Recent Reviews

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 Verified Customer
01/08/2015
Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol IRF1
Uniprot