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Neprilysin-2/MMEL1 Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2340
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Catalog# & Formulation Size Price
Carrier Free
AF2340-SP
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Neprilysin-2/MMEL1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neprilysin-2/MMEL1
Gly69-Trp770
Accession # AAL08942
Specificity
Detects human Neprilysin-2/MMEL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Neprilysin is observed and less than 1% cross-reactivity with rhECE-1 and rhECE-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMEL1
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2340 in the following applications:
Publications
Read Publication using AF2340.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neprilysin-2/MMEL1 Antibody

  • EC 3.4.24
  • EC 3.4.24.11
  • MELL1
  • membrane metallo-endopeptidase-like 1
  • Membrane metallo-endopeptidase-like 2MGC119455
  • MGC119454
  • MMEL1
  • MMEL2
  • NEP2
  • NEP2(m)
  • NEPIIMGC119456
  • Neprilysin II
  • Neprilysin2
  • Neprilysin-2
  • NL2
  • NL2NL1
  • SEP
  • soluble secreted endopeptidase
  • zinc metallopeptidase

Background

Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles. The ectodomain of human Neprilysin-2/MMEL1 was expressed with an N-terminal His tag and purified.

  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Carpentier, M. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 426.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neprilysin-2/MMEL1 Antibody (AF2340)(1)


Review for Neprilysin-2/MMEL1 Antibody (AF2340) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2340:
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 WB Human 01/06/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22008264
SpeciesHuman

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Application: WB
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Bioinformatics

Gene Symbol MMEL1
Uniprot