Home » Neprilysin-2/MMEL1 » Neprilysin-2 Antibodies » Neprilysin-2/MMEL1 Antibody
Novus Biologicals products are now on bio-techne.com

Neprilysin-2/MMEL1 Antibody

 (1 Publication)
 (1 Review)
 See All Related
Submit a Review

Images

 
There are currently no images for Neprilysin-2/MMEL1 Antibody (AF2340).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2340
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2340-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Neprilysin-2/MMEL1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neprilysin-2/MMEL1
Gly69-Trp770
Accession # AAL08942
Specificity
Detects human Neprilysin-2/MMEL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Neprilysin is observed and less than 1% cross-reactivity with rhECE-1 and rhECE-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMEL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2340 in the following applications:
Publications
Read Publication using AF2340.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neprilysin-2/MMEL1 Antibody

  • EC 3.4.24
  • EC 3.4.24.11
  • MELL1
  • membrane metallo-endopeptidase-like 1
  • Membrane metallo-endopeptidase-like 2MGC119455
  • MGC119454
  • MMEL1
  • MMEL2
  • NEP2
  • NEP2(m)
  • NEPIIMGC119456
  • Neprilysin II
  • Neprilysin2
  • Neprilysin-2
  • NL2
  • NL2NL1
  • SEP
  • soluble secreted endopeptidase
  • zinc metallopeptidase

Background

Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles. The ectodomain of human Neprilysin-2/MMEL1 was expressed with an N-terminal His tag and purified.

  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Carpentier, M. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 426.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

IRF1 Antibody
[Unconjugated]
AF4715
Western Blot IRF1 Antibody [Unconjugated]
Western Blot IRF1 Antibody [Unconjugated]
(1 Review)
(2 Publications)
Species: Mu
Applications: WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
PMEL17/SILV
Antibody (HMB45)
NBP2-44520
Flow (Intracellular) PMEL17/SILV Antibody (HMB45)
Flow (Intracellular) PMEL17/SILV Antibody (HMB45)
Immunohistochemistry PMEL17/SILV Antibody (HMB45)
(8 Publications)
Species: Ca(-), Hu, Rt(-)
Applications: Flow-IC, Flow, ICC/IF, IF, IHC, IHC-P, MI, WB
Cathepsin L
Antibody [Unconjugate
AF952
Western Blot Cathepsin L Antibody [Unconjugated]
Immunohistochemistry Cathepsin L Antibody [Unconjugated]
Western Blot Cathepsin L Antibody [Unconjugated]
(27 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
Neurolysin
Antibody (OTI1D6) - A ...
NBP2-72961
Western Blot Neurolysin Antibody (OTI1D6) - Azide and BSA Free
Western Blot Neurolysin Antibody (OTI1D6) - Azide and BSA Free
Flow Cytometry Neurolysin Antibody (OTI1D6) - Azide and BSA Free
Species: Hu, Pm, Mu, Pm, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, WB
ENDOGL1 Antibody
(2F7)
H00009941-M02
Immunocytochemistry/ Immunofluorescence ENDOGL1 Antibody (2F7)
Immunohistochemistry-Paraffin ENDOGL1 Antibody (2F7)
Western Blot ENDOGL1 Antibody (2F7)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
SUB1 Antibody
NBP1-82454
Immunohistochemistry-Paraffin SUB1 Antibody
Immunohistochemistry-Paraffin SUB1 Antibody
Immunohistochemistry-Paraffin SUB1 Antibody
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
SF3B14 Antibody
NBP1-87431
Immunocytochemistry/ Immunofluorescence SF3B14 Antibody
Immunohistochemistry-Paraffin SF3B14 Antibody
Immunohistochemistry-Paraffin SF3B14 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
ROBLD3 Antibody -
BSA Free
NBP2-81696
Western Blot ROBLD3 Antibody - BSA Free
Immunohistochemistry ROBLD3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence ROBLD3 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
S100A9 Antibody
[Unconjugated]
AF2065
Immunocytochemistry S100A9 Antibody [Unconjugated]
Immunohistochemistry S100A9 Antibody [Unconjugated]
Immunocytochemistry S100A9 Antibody [Unconjugated]
(2 Reviews)
(34 Publications)
Species: Mu
Applications: ICC, IHC, Simple Western, WB
RPP14 Antibody
NBP1-80965
Western Blot RPP14 Antibody
Immunocytochemistry/ Immunofluorescence RPP14 Antibody
Immunohistochemistry-Paraffin RPP14 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CDK2AP2 Antibody
H00010263-B01P
Western Blot CDK2AP2 Antibody
Western Blot CDK2AP2 Antibody
Western Blot CDK2AP2 Antibody
Species: Hu, Mu, Rt
Applications: WB
Cathepsin C/DPPI
Antibody [Unconjugate ...
AF1034
Western Blot Cathepsin C/DPPI Antibody [Unconjugated]
Immunohistochemistry Cathepsin C/DPPI Antibody [Unconjugated]
Western Blot Cathepsin C/DPPI Antibody [Unconjugated]
(13 Publications)
Species: Mu, Rt
Applications: IHC, Simple Western, WB
Exosome component
10 Antibody
NBP1-32870
Western Blot Exosome component 10 Antibody
Immunohistochemistry-Paraffin Exosome component 10 Antibody
Western Blot Exosome component 10 Antibody
(2 Reviews)
(3 Publications)
Species: Hu
Applications: IHC, IHC-P, IP, KD, WB
NOC2L Antibody
NBP1-92190
Immunocytochemistry/ Immunofluorescence NOC2L Antibody
Western Blot NOC2L Antibody
Immunohistochemistry-Paraffin NOC2L Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, Simple Western, WB
B3GALNT1 Antibody
- BSA Free
NBP2-92677
Western Blot B3GALNT1 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: WB
Protamine 1
Antibody
NBP2-30949
Immunohistochemistry-Paraffin Protamine 1 Antibody
Immunohistochemistry-Paraffin Protamine 1 Antibody
Immunohistochemistry-Paraffin Protamine 1 Antibody
Species: Hu
Applications: IHC, IHC-P
NUCKS1 Antibody
NB100-74635
Immunohistochemistry-Paraffin NUCKS1 Antibody
Immunoprecipitation NUCKS1 Antibody
Species: Hu
Applications: IHC, IHC-P, IP, WB (-)
Perforin Antibody
(CB5.4) - BSA Free
NBP1-97512
Western Blot Perforin Antibody (CB5.4) - BSA Free
(7 Publications)
Species: Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB

Publications for Neprilysin-2/MMEL1 Antibody (AF2340)(1)


Review for Neprilysin-2/MMEL1 Antibody (AF2340) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2340:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22008264
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Neprilysin-2/MMEL1 Antibody (AF2340) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Neprilysin-2/MMEL1 Products

Blogs on Neprilysin-2/MMEL1

There are no specific blogs for Neprilysin-2/MMEL1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0
 
 Verified Customer
01/06/2015
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol MMEL1
Uniprot