![Western Blot: IRF9 Antibody [NBP2-16991] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF9 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IRF9-Antibody-Western-Blot-NBP2-16991-img0008.jpg "Western Blot: IRF9 Antibody [NBP2-16991] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF9 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, RMSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IRF9. The exact sequence is proprietary. |
| Localization | Cytoplasm, Nucleus |
| Predicted Species | Rhesus Macaque (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | IRF9 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA usage reported in scientific literature (PMID: 27381292). |
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| Theoretical MW | 44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Immunogen affinity purified |
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| Publication using NBP2-16991 | Applications | Species |
|---|---|---|
| Assetta B, De Cecco M, O'Hara B, Atwood WJ. JC Polyomavirus Infection of Primary Human Renal Epithelial Cells Is Controlled by a Type I IFN-Induced Response MBio 2016-07-06 [PMID: 27381292] (ELISA) | ELISA |
Secondary Antibodies |
Isotype Controls |
Research Areas for IRF9 Antibody (NBP2-16991)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: IRF9 Antibody [NBP2-16991] - Paraformaldehyde-fixed HeLa cells, using IRF9 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/IRF9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16991-img0003.jpg "Immunocytochemistry/Immunofluorescence: IRF9 Antibody [NBP2-16991] - Paraformaldehyde-fixed HeLa cells, using IRF9 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: IRF9 Antibody [NBP2-16991] - Human breast cancer, using IRF9 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/IRF9-Antibody-Immunohistochemistry-Paraffin-NBP2-16991-img0002.jpg "Immunohistochemistry-Paraffin: IRF9 Antibody [NBP2-16991] - Human breast cancer, using IRF9 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: IRF9 Antibody [NBP2-16991] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IRF9 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IRF9-Antibody-Western-Blot-NBP2-16991-img0006.jpg "Western Blot: IRF9 Antibody [NBP2-16991] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IRF9 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")