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![Western Blot: IRF1 Antibody (BLR039F) [NBP2-76419] - Detection of human IRF1 by WB of Jurkat, Hep-G2, HeLa (IFNgamma treated), HeLa, SR, MOLT-4, RPMI-8226, KG-1, A-549, and MCF-7 lysate. Antibody: Rabbit anti-IRF1 recombinant monoclonal [BLR039F] (NBP2-76419). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/IRF1-Antibody-BLR039F-Western-Blot-NBP2-76419-img0003.jpg "Western Blot: IRF1 Antibody (BLR039F) [NBP2-76419] - Detection of human IRF1 by WB of Jurkat, Hep-G2, HeLa (IFNgamma treated), HeLa, SR, MOLT-4, RPMI-8226, KG-1, A-549, and MCF-7 lysate. Antibody: Rabbit anti-IRF1 recombinant monoclonal [BLR039F] (NBP2-76419). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![Immunocytochemistry: IRF1 Antibody (BLR039F) [NBP2-76419] - Detection of human IRF1 in FFPE OVCAR-8 cells by ICC. Antibody: Rabbit anti-IRF1 recombinant monoclonal [BLR039F] (NBP2-76419). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/IRF1-Antibody-BLR039F-Immunocytochemistry-NBP2-76419-img0002.jpg)
![Immunohistochemistry: IRF1 Antibody (BLR039F) [NBP2-76419] - Detection of human IRF1 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-IRF1 recombinant monoclonal [BLR039F] (NBP2-76419). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/IRF1-Antibody-BLR039F-Immunohistochemistry-NBP2-76419-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: IRF1 Antibody (BLR039F) [NBP2-76419] - Detection of human IRF1 by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-IRF1 recombinant monoclonal [BLR039F] (NBP2-76419) and another rabbit anti-IRF1 recombinant monoclonal.](http://images.novusbio.com/fullsize/IRF1-Antibody-BLR039F-Immunoprecipitation-NBP2-76419-img0004.jpg)
![Western Blot: IRF1 Antibody (2E4) [H00003659-M01] - IRF1 monoclonal antibody (M01), clone 2E4 Analysis of IRF1 expression in COLO 320 HSR.](http://images.novusbio.com/fullsize/IRF1-Antibody-2E4-Western-Blot-H00003659-M01-img0004.jpg "Western Blot: IRF1 Antibody (2E4) [H00003659-M01] - IRF1 monoclonal antibody (M01), clone 2E4 Analysis of IRF1 expression in COLO 320 HSR.")
![Sandwich ELISA: IRF1 Antibody (2E4) [H00003659-M01] - Detection limit for recombinant GST tagged IRF1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IRF1-Antibody-2E4-Sandwich-ELISA-H00003659-M01-img0003.jpg)
![Western Blot: IRF1 Antibody [NBP1-78763] - Whole cell lysate from NIH3T3 (M; 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-IRF1 antibody Aused for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78761 and NBP1-78762, which recognize disparate epitopes.](http://images.novusbio.com/fullsize/IRF1-Antibody-Western-Blot-NBP1-78763-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: IRF1 Antibody [NBP1-78763] - Whole cell lysate from NIH3T3 (M; 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-IRF1 antibody Aused for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78761 and NBP1-78762, which recognize disparate epitopes.")
![Western Blot: IRF1 Antibody [NBP1-78761] - Whole cell lysate from NIH3T3 cells (M; 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified rabbit anti-IRF1 antibody used for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78762 and NBP1-78763 which recognize downstream epitopes.](http://images.novusbio.com/fullsize/IRF1-Antibody-Western-Blot-NBP1-78761-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: IRF1 Antibody [NBP1-78761] - Whole cell lysate from NIH3T3 cells (M; 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified rabbit anti-IRF1 antibody used for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78762 and NBP1-78763 which recognize downstream epitopes.")
![Immunohistochemistry-Paraffin: IRF1 Antibody [NBP2-49384] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/IRF1-Antibody-Immunohistochemistry-NBP2-49384-img0001.jpg "Immunohistochemistry-Paraffin: IRF1 Antibody [NBP2-49384] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.")
![Western Blot: IRF1 Overexpression Lysate (Adult Normal) [NBL1-12030] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRF1.](http://images.novusbio.com/fullsize/IRF1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12030-img0002.jpg "Western Blot: IRF1 Overexpression Lysate (Adult Normal) [NBL1-12030] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRF1.")
![Western Blot: IRF1 Antibody (1C1) [H00003659-M02] - Detection against Immunogen (37.84 KDa) .](http://images.novusbio.com/fullsize/IRF1-Antibody-1C1-Western-Blot-H00003659-M02-img0001.jpg "Western Blot: IRF1 Antibody (1C1) [H00003659-M02] - Detection against Immunogen (37.84 KDa) .")
![Immunoprecipitation: IRF1 Antibody [NBP1-78762] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from NIH3T3 cells. Affinity purified rabbit anti-IRF1 antibody used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78761 and NBP1-78763, which recognize disparate epitopes.](http://images.novusbio.com/fullsize/IRF1-Antibody-Immunoprecipitation-NBP1-78762-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: IRF1 Antibody [NBP1-78762] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from NIH3T3 cells. Affinity purified rabbit anti-IRF1 antibody used for IP at 6 mcg/mg lysate. IRF1 was also immunoprecipitated by rabbit anti-IRF1 antibodies NBP1-78761 and NBP1-78763, which recognize disparate epitopes.")