![Western Blot: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIHx2011;3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 ug/mL mouse anti-IkB-a monoclonal (NB100-56507, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Azide-and-BSA-Free-Western-Blot-NBP2-80787-img0003.jpg "Western Blot: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Lysates of Jurkat human acute T cell leukemia cell line, LNCaP human prostate cancer cell line, PCx2011;3 human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIHx2011;3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 ug/mL mouse anti-IkB-a monoclonal (NB100-56507, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC, IP, CyTOF-ready, KO |
| Clone | 6A920 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NFKBIA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size. This antibody is CyTOF ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for IkB-alpha Antibody (NBP2-80787)Find related products by research area.
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Sample collection from mammalian culture cells for kinomic analysis By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit... Read full blog post. |
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The effects of curcumin on IKB Alpha and the NFkB signaling pathway The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta. These kinases are at the core of the NFkB signaling cascade. The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through... Read full blog post. |
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Inhibitor kappa B-alpha (IkappaB-alpha) The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NFKBIA |
![Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-IkB-alpha (6A920) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80787-img0004.jpg "Immunocytochemistry/Immunofluorescence: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-IkB-alpha (6A920) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Immunohistochemistry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Human breast probed with IkBa antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80787-img0005.jpg "Immunohistochemistry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Human breast probed with IkBa antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min")
![Flow Cytometry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Intracellular staining of 10^6 ThP-1 cells using 0.25 ug of NB100-56507. Shaded histogram represents cells alone, green represents the isotype control, and red represents the IkBa antibody. Novus's intracellular flow kit was used for this test, and an anti-mouse IgG FITC conjugated secondary. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80787-img0008.jpg "Flow Cytometry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Intracellular staining of 10^6 ThP-1 cells using 0.25 ug of NB100-56507. Shaded histogram represents cells alone, green represents the isotype control, and red represents the IkBa antibody. Novus's intracellular flow kit was used for this test, and an anti-mouse IgG FITC conjugated secondary. Image from the standard format of this antibody.")
![Western Blot: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - IkBa using NB100-56507 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Azide-and-BSA-Free-Western-Blot-NBP2-80787-img0001.jpg "Western Blot: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - IkBa using NB100-56507 at 2 ug/ml in (A) Daudi and (B) NIH 3T3 whole cell lysate. Image from the standard format of this antibody.")
![Western Blot: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NB100-56724 (lanes 1 & 2) or NB100-56507 (that recognizes both non-phospho and phosphorylated forms of IkBa) and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Azide-and-BSA-Free-Western-Blot-NBP2-80787-img0002.jpg "Western Blot: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NB100-56724 (lanes 1 & 2) or NB100-56507 (that recognizes both non-phospho and phosphorylated forms of IkBa) and immunoreactivity was detected by ECL. The data shows that NB100-56724 detects specifically the phosphorylated form of IkBa. Image from the standard format of this antibody.")
![Flow Cytometry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - An intracellular stain was performed on Hek293 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80787-img0006.jpg "Flow Cytometry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - An intracellular stain was performed on Hek293 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image from the standard format of this antibody.")
![Flow Cytometry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - An intracellular stain was performed on NIH3T3 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80787-img0007.jpg "Flow Cytometry: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - An intracellular stain was performed on NIH3T3 cells with IkB-alpha Antibody [6A920] NB100-56507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550. Image from the standard format of this antibody.")
![Simple Western: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Simple Western lane view shows a specific band for IKB alpha in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Azide-and-BSA-Free-Simple-Western-NBP2-80787-img0010.jpg "Simple Western: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Simple Western lane view shows a specific band for IKB alpha in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.")
![Knockout Validated: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/IkB-alpha-Antibody-6A920-Azide-and-BSA-Free-Knockout-Validated-NBP2-80787-img0009.jpg "Knockout Validated: IkB-alpha Antibody (6A920) - Azide and BSA Free [NBP2-80787] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and IkB-alpha knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human IkB-alpha monoclonal Antibody (Catalog # NB100-56507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific band was detected for IkB-alpha at approximately 38 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")