IkB-alpha Antibody (6A920) - BSA Free

IkBa Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses IkBa becomes phosphorylated, promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IHC-P, IP, Simple Western, WB.

Showing Publications 1 - 10 of 42. Show All 42 Publications. Collapse Publications.

Publications using NB100-56507	Applications	Species
Lan Zhao, Yumei Lai, Hongli Jiao, Jian Huang Nerve growth factor receptor limits inflammation to promote remodeling and repair of osteoarthritic joints Nature Communications 2024-04-15 [PMID: 38622181]
Zeng L, Herdman DS, Lee SM et al. Loss of cAMP signaling in CD11c immune cells protects against diet-induced obesity Diabetes 2023-05-31 [PMID: 37257047] (WB)	WB
S Ohta, M Asanoma, N Irie, N Tachibana, M Kohno Soy Phospholipids Exert a Renoprotective Effect by Inhibiting the Nuclear Factor Kappa B Pathway in Macrophages Metabolites, 2022-04-06;12(4):. 2022-04-06 [PMID: 35448517] (Simple Western)	Simple Western
Sartori G, Napoli S, Cascione L et al. ASB2 is a direct target of FLI1 that sustains NF-?B pathway activation in germinal center-derived diffuse large B-cell lymphoma Journal of Experimental & Clinical Cancer Research 2021-11-11 [PMID: 34763718] (WB)	WB
Stanfield BA, Purves T, Palmer S, et al. IL-10 and class 1 histone deacetylases act synergistically and independently on the secretion of proinflammatory mediators in alveolar macrophages PloS one 2021-01-20 [PMID: 33471802] (WB, Mouse)	WB	Mouse
Korgaonkar AA, Nguyen S, Li Y et al. Distinct cellular mediators drive the Janus faces of toll-like receptor 4 regulation of network excitability which impacts working memory performance after brain injury Brain Behav. Immun. 2020-04-04 [PMID: 32259563] (WB, Rat)	WB	Rat
Kamohara A, Hirata H et al. IgG immune complexes with Staphylococcus aureus protein A enhance osteoclast differentiation and bone resorption by stimulating Fc receptors and TLR2. Int Immunol 2019-12-11 [PMID: 31713625] (WB, Mouse)	WB	Mouse
Chen Z, Yao L, Liu Y et al. Astragaloside IV regulates NF-kappa B mediated cellular senescence and apoptosis of hepatic stellate cells to suppress PDGF BB induced activation Exp Ther Med 2019-09-25 [PMID: 31641375] (WB, Rat)	WB	Rat
Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human) Details: Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).		Human
Zhu F, Willette-Brown J, Song NY et al. Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis. Cell Host Microbe. 2017-04-12 [PMID: 28407484]
Tomasi ML, Ramani K, Ryoo M. Ubiquitin-Conjugating Enzyme 9 Phosphorylation as a Novel Mechanism for Potentiation of the Inflammatory Response. Am. J. Pathol. 2016-09-01 [PMID: 27561301] (WB)	WB
Gao Q, Liu Y, Wu Y et al. IL-17 intensifies IFN-gamma-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-kB Int. J. Mol. Med. 2015-12-11 [PMID: 26677135] (WB, Mouse)	WB	Mouse
Hochrainer K, Pejanovic N, Olaseun VA et al. The ubiquitin ligase HERC3 attenuates NF-kB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation. Nucleic Acids Res. 2015-10-17 [PMID: 26476452] (WB, Human)	WB	Human
Ikeno H, Apel M, Zouboulis C et al. L-Ascorbyl-2-phosphate attenuates NF-kB signaling in SZ95 sebocytes without affecting IL-6 and IL-8 secretion Arch. Dermatol. Res. 2015-04-18 [PMID: 25894228] (WB, Human)	WB	Human
Yang XY, Zhao N, Liu YY et al. Inhibition of NADPH Oxidase Mediates Protective Effect of Cardiotonic Pills against Rat Heart Ischemia/Reperfusion Injury. Evid Based Complement Alternat Med 2013-01-01 [PMID: 23840265] (IHC-P, Rat)	IHC-P	Rat
Tabit CE, Shenouda SM, Holbrook M et al. Protein Kinase C-beta Contributes to Impaired Endothelial Insulin Signaling in Humans With Diabetes Mellitus Circulation 2013-01-01 [PMID: 23204109] (ICC/IF, Human)	ICC/IF	Human
Pejanovic N, Hochrainer K, Liu T et al. Regulation of nuclear factor kB (NF-kB) transcriptional activity via p65 acetylation by the chaperonin containing TCP1 (CCT). PLoS One. 2012-01-01 [PMID: 22860050]
Delhase M, Kim SY, Lee H et al. TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2. Proc Natl Acad Sci U S A. 2012-01-24 [PMID: 22203995]
Harikumar KB, Sung B, Pandey MK et al. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway Mol Pharmacol 2010-05-01 [PMID: 20103608] Details: This citation used the Alexa Fluor 488 version of this antibody.
Yu Ming-Can, Su Liu-Li, Zou Lin et al. An essential function for beta-arrestin 2 in the inhibitory signaling of natural killer cells. Nat Immunol. 2008-08-01 [PMID: 18604210] (Mouse)		Mouse
Nishioka C, Ikezoe T, Jing Y et al. DHMEQ, a novel nuclear factor-kappaB inhibitor, induces selective depletion of alloreactive or phytohaemagglutinin-stimulated peripheral blood mononuclear cells, decreases production of T helper type 1 cytokines, and blocks maturation of dendritic cells. Immunology. 2008-06-01 [PMID: 18217958]
Shakibaei M, John T, Schulze-Tanzil G et al. Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis. Biochem Pharmacol. 2007-05-01 [PMID: 17291458]
Vilimas T, Mascarenhas J, Palomero T et al. Targeting the NF-kappaB signaling pathway in Notch1-induced T-cell leukemia. Nat Med. 2007-01-01 [PMID: 17173050] (IP, WB) Details: IP (CEM cells treated with Z-Leu-Leu-Nle-CHO and LY411575), Fig. 2d WB (CEM cells IP'd with Ikk antibody or Notch1-IC antibody), Figs. 2e, 2f.	IP, WB
Endo T, Nishio M, Enzler T et al. BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway. Blood. 2007-01-15 [PMID: 16973958] (WB, Human) Details: WB (human CLL B cells cultured with rhBAFF or rhAPRIL), Fig. 2b WB (CLL cells transfected with HA-tagged IkBaSR or empty pcDNA3 vector stimulated with rhBAFF), Fig. 5a.	WB	Human
Ishiguro K, Ando T, Maeda O et al. Paeonol attenuates TNBS-induced colitis by inhibiting NF-kappaB and STAT1 transactivation. Toxicol Appl Pharmacol. 2006-11-15 [PMID: 16928387] (WB, Mouse) Details: WB (mouse colon tissue CW-2 cells stimulated with TNFa in presence of Paeonol) Fig. 5a	WB	Mouse
Santhi WS, Sebastian P, Varghese BT et al. NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins. Exp Mol Pathol. 2006-10-01 [PMID: 16822500] Details: Products used (human): 1. IkBa [IMG-127A]: IHC paraffin (normal oral mucosa, leukoplakia, and oral cancer), Fig. 1 IHC paraffin analysis (normal oral mucosa, leukoplakia, and oral cancer), Fig. 2 IHC paraffin (oral cancer: primary tumor, surgical margin,
Macagno A, Molteni M, Rinaldi A et al. A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression. J Exp Med. 2006-06-12 [PMID: 16717116] (WB)	WB
Strozyk E, P0ppelmann B, Schwarz T et al. Differential effects of NF-kappaB on apoptosis induced by DNA-damaging agents: the type of DNA damage determines the final outcome. Oncogene. 2006-10-12 [PMID: 16702954] (WB, Human) Details: WB (human oral epithelial carcinoma cell line kB) Figs. 1a, 5b, 5d.	WB	Human
Perez-Moreno M, Davis MA, Wong E et al. p120-catenin mediates inflammatory responses in the skin. Cell. 2006-02-10 [PMID: 16469707] (WB, Mouse) Details: WB (wt and p120 null mouse epidermis), Fig. 7c	WB	Mouse
Chang L, Kamata H, Solinas G et al. The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover. Cell. 2006-02-10 [PMID: 16469705] (WB) Details: WB: 1. IkBa [IMG-127A], (wt hepatocytes incubated with proteasome inhibitor MG-132 and TNFa or CHX added), Fig. 3a 2. Ubiquitin [IMG-5021]: (wt and Jnk1-/- injected mouse liver with ConA), Fig. 3b (wt mouse liver injected with LPS and Cal-N and JNk-inhibi	WB
Yang M, Hase H, Legarda-Addison D et al. B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells. J Immunol. 2005-09-01 [PMID: 16116167] (WB) Details: Antibodies cited [WB]: 1. IkBa Ser 32/Ser 36 [IMG-156A], (A20 B cells stimulated with APRIL), Fig. 2c 2. IkBa [IMG-127A], (A20 cells stimulated with anti-TNFR2), Fig. 6a	WB
Napolitani G, Rinaldi A, Bertoni F et al. Selected Toll-like receptor agonist combinations synergistically trigger a T helper type 1-polarizing program in dendritic cells. Nat Immunol. 2005-08-01 [PMID: 15995707] (WB, Human) Details: WB (human MoDC stimulated with LPS, R848, and poly(I:C)), Fig. 8b.	WB	Human
Bernasconi D, Amici C, La Frazia S et al. The IkappaB kinase is a key factor in triggering influenza A virus-induced inflammatory cytokine production in airway epithelial cells. J Biol Chem. 2005-06-24 [PMID: 15837793] (WB, Human) Details: WB (WSN-infected A549 human pulmonary cells), Fig. 1a bottom	WB	Human
Xia YF, Ye BQ, Li YD et al. Andrographolide attenuates inflammation by inhibition of NF-kappa B activation through covalent modification of reduced cysteine 62 of p50. J Immunol. 2004-09-15 [PMID: 15356172] (WB)	WB
Saccani S, Marazzi I, Beg AA, Natoli G. Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kappaB response. J Exp Med. 2004-07-05 [PMID: 15226358] (WB)	WB
Ikezoe T, Yang Y, Heber D et al. PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock. Mol Pharmacol. 2003-12-01 [PMID: 14645683] (WB)	WB
Saccani S, Pantano S, Natoli G. Modulation of NF-kappaB activity by exchange of dimers. Mol Cell. 2003-06-01 [PMID: 12820969] (WB)	WB
Goodkin ML, Ting AT, Blaho JA. NF-kappaB is required for apoptosis prevention during herpes simplex virus type 1 infection. J Virol. 2003-07-01 [PMID: 12805425] (WB) Details: WB (mouse HEp-2 cells and clonal CD-14 transfected cells treated with either TNF-a or TNF-a plus CHX), Fig. 9a WB (mouse HEp-2 cells treated with TNF-a plus CHX and mutant IkBaDN cells treated with TNF-a), Fig. 9b WB (mouse mutant HEp-2 and IkBaDN cells i	WB
Han HS, Karabiyikoglu M, Kelly S et al. Mild hypothermia inhibits nuclear factor-kappaB translocation in experimental stroke. J Cereb Blood Flow Metab. 2003-05-01 [PMID: 12771574] Details: Products cited for western blot in male Sprague-Dawley rats ischemic cortex, ischemic subcortex, contralateral cortex, and contralateral subcortex tissue. The rats were subject to mild and delayed hypothermia. 1. IKK-gamma (IMG-324A): Fig 4A & 4B. 2. IkB-
Delhase Mireille. IkappaB kinase and NF-kappaB signaling in response to pro-inflammatory cytokines. Methods Mol Biol. 2003-01-01 [PMID: 12769469]
Saccani S, Pantano S, Natoli G. p38-Dependent marking of inflammatory genes for increased NF-kappa B recruitment. Nat Immunol. 2002-01-01 [PMID: 11743587] (WB, Human)	WB	Human
Saccani S, Pantano S, Natoli G. Two waves of nuclear factor kappaB recruitment to target promoters. J Exp Med. 2001-06-18 [PMID: 11413190] (WB, Mouse)	WB	Mouse
Show All 42 Publications. Collapse Publications.

Array NB100-56507

• IkB-alpha Antibodies
• IkB-alpha Antibody Pair
• IkB-alpha Lysate
• IkB-alpha Proteins
• IkB-alpha ELISA