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IkB-alpha Antibody (6A920) [DyLight 594]

 
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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP, CyTOF-ready, KO
Clone
6A920
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 594

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56507AF350
Alexa Fluor 405
NB100-56507AF405
Alexa Fluor 488
NB100-56507AF488
Alexa Fluor 532
NB100-56507AF532
Alexa Fluor 594
NB100-56507AF594
Alexa Fluor 647
NB100-56507AF647
Alexa Fluor 700
NB100-56507AF700
Alexa Fluor 750
NB100-56507AF750
Allophycocyanin
NB100-56507APC
Allophycocyanin/Cy7
NB100-56507APCCY7
Biotin
NB100-56507B
Biotin
NBP2-24815
DyLight 350
NB100-56507UV
DyLight 405
NB100-56507V
DyLight 488
NB100-56507G
DyLight 550
NB100-56507R
DyLight 650
NB100-56507C
DyLight 680
NB100-56507FR
DyLight 755
NB100-56507IR
FITC
NB100-56507F
HRP
NB100-56507H
Janelia Fluor 549
NB100-56507JF549
Janelia Fluor 646
NB100-56507JF646
PE
NB100-56507PE
PE/Atto594
NB100-56507PEATT594
PE/Cy5.5
NB100-56507PECY55
PE/Cy7
NB100-56507PECY7
PerCP
NB100-56507PCP
Unconjugated
NB100-56507
mFluor Violet 450 SE
NB100-56507MFV450
mFluor Violet 500 SE
NB100-56507MFV500
mFluor Violet 610 SE
NB100-56507MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-56507
BSA Free
NB100-56507SS
Azide and BSA Free
NBP2-80787
BSA Free
NB100-56507
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IkB-alpha Antibody (6A920) [DyLight 594] Summary

Immunogen
A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIA
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 23840265).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for IkB-alpha Antibody (6A920) [DyLight 594]

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • NFKBI
  • NFKBIA
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NFKBIA