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WNK1 Antibody [Unconjugated]

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Western blot shows lysates of DA3 mouse myeloma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat WNK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2849) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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WNK1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant rat WNK1
Ser816-Ser1173
Accession # Q9JIH7
Specificity
Detects mouse and rat WNK1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
WNK1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2849 in the following application:

Publications
Read Publications using
AF2849 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WNK1 Antibody [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.1
  • Erythrocyte 65 kDa protein
  • HSAN2hereditary sensory neuropathy, type II
  • HSN2MGC163339
  • hWNK1
  • KDP
  • KDPMGC163341
  • KIAA0344
  • Kinase deficient protein
  • p65
  • PHA2C
  • PRKWNK1
  • PRKWNK1PSK
  • prostate-derived sterile 20-like kinase
  • Protein kinase lysine-deficient 1
  • Protein kinase with no lysine 1
  • protein kinase, lysine deficient 1
  • serine/threonine-protein kinase WNK1
  • WNK lysine deficient protein kinase 1
  • WNK1

Background

With No Lysine (K) 1 (WNK1) belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen excretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WNK1 Antibody (AF2849)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


Filter By Application
Flow Cytometry
(1)
Western Blot
(3)
All Applications
Filter By Species
Mouse
(4)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF2849 Applications Species
Trzeciak, AJ;Rojas, WS;Liu, ZL;Krebs, AS;Wang, Z;Saavedra, PHV;Miranda, IC;Lipshutz, A;Xie, J;Huang, CL;Overholtzer, M;Glickman, MS;Parkhurst, CN;Vierbuchen, T;Lucas, CD;Perry, JSA; WNK1 enforces macrophage lineage fidelity bioRxiv : the preprint server for biology 2023-04-28 [PMID: 37383948] (Western Blot, Mouse) Western Blot Mouse
Jin X, Xie J, Yeh CW et al. WNK1 promotes water homeostasis by acting as a central osmolality sensor for arginine vasopressin release The Journal of clinical investigation 2023-04-18 [PMID: 37071482]
B Saha, DCAL Dellova, J Demko, MV Sørensen, E Takagi, CE Gleason, W Shabbir, D Pearce WNK1 is a chloride-stimulated scaffold that regulates mTORC2-activity and ion transport Journal of Cell Science, 2022-12-05;0(0):. 2022-12-05 [PMID: 36373794] (Western Blot, Mouse) Western Blot Mouse
S Ishihara, T Sato, G Du, D Guardavacc, A Nakajima, S Sawai, T Kataoka, K Katagiri Phosphatidic acid-dependent localization and basal de-phosphorylation of RA-GEFs regulate lymphocyte trafficking BMC Biol., 2020-06-29;18(1):75. 2020-06-29 [PMID: 32600317] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Shekarabi M, Girard N, Riviere JB, Dion P, Houle M, Toulouse A, Lafreniere RG, Vercauteren F, Hince P, Laganiere J, Rochefort D, Faivre L, Samuels M, Rouleau GA Mutations in the nervous system--specific HSN2 exon of WNK1 cause hereditary sensory neuropathy type II. J. Clin. Invest., 2008-07-01;118(7):2496-505. 2008-07-01 [PMID: 18521183] (Western Blot, Mouse) Western Blot Mouse

Review for WNK1 Antibody (AF2849) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2849:
Filter by Applications
Immunofluorescence staining
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
Immunofluorescence staining Human 12/07/2023
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Summary

ApplicationOther
Sample TestedHuman Dermal Microvascular Endothelial Cells - fixed with PFA
SpeciesHuman
LotVNP032311A

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***

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Verified Customer
12/07/2023
Application: Immunofluorescence staining
Species: Human

Bioinformatics

Gene Symbol WNK1
Uniprot