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GR/NR3C1 Antibody - Azide and BSA Free

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Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in Hela Cells. Glucocorticoid ...read more
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in A2058 Cells. Glucocorticoid ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, GS, ICC/IF, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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GR/NR3C1 Antibody - Azide and BSA Free Summary

Immunogen
Synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR.
Localization
Cytoplasmic in the absence of ligand; nuclear after ligand-binding.
Specificity
NB120-3579 detects glucocorticoid receptor (GR) from human, mouse and rat tissues.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR3C1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Frozen 1 ug/ml
  • Immunoprecipitation 5 ug/ml
  • Simple Western
  • Western Blot 5 ug/ml
Application Notes
WB: Detects an approx. 97 kDa protein representing GR as well as two other unidentified proteins at approx. 135 - 145 kDa from rat liver extract. IHC: Staining of GR in rat hippocampus using NB120-3579 results in staining primarily in the nucleus. NB120-3579 does not disrupt receptor-DNA interactions.
See Simple Western Antibody Database for Simple Western validation: tested in primate corpus luteum; antibody dilution of 1:10; separated by size; multiple isoform bands detected

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS.

Alternate Names for GR/NR3C1 Antibody - Azide and BSA Free

  • GCR
  • glucocorticoid receptor
  • GR
  • GRGCCR
  • GRLNuclear receptor subfamily 3 group C member 1
  • NR3C1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1

Background

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GR/NR3C1 Antibody (NB120-3579) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NR3C1
Entrez
Uniprot