Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
Applications | WB, Simple Western, GS, ICC/IF, IHC, IP, ChIP |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Format | Azide and BSA Free |
Concentration | LYOPH |
Immunogen | Synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR. |
Localization | Cytoplasmic in the absence of ligand; nuclear after ligand-binding. |
Specificity | NB120-3579 detects glucocorticoid receptor (GR) from human, mouse and rat tissues. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | NR3C1 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
Application Notes | WB: Detects an approx. 97 kDa protein representing GR as well as two other unidentified proteins at approx. 135 - 145 kDa from rat liver extract. IHC: Staining of GR in rat hippocampus using NB120-3579 results in staining primarily in the nucleus. NB120-3579 does not disrupt receptor-DNA interactions. See Simple Western Antibody Database for Simple Western validation: tested in primate corpus luteum; antibody dilution of 1:10; separated by size; multiple isoform bands detected |
Storage | Store at -20C. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | Immunogen affinity purified |
Reconstitution Instructions | Reconstitute with PBS. |
Secondary Antibodies |
Isotype Controls |
Research Areas for GR/NR3C1 Antibody (NB120-3579)Find related products by research area.
|
Epigenetics of Depression: How Can Psychological Stress Alter Your DNA? By Emily Cartwright, PhDHow Can Psychological Stress Alter Your DNA? Traumatic events, work demands, relationship conflicts, and health problems are all examples of psychological stressors that can result in phy... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.