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ENPP-3/CD203c Antibody (NP4D6) - BSA Free

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Immunocytochemistry/Immunofluorescence: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, green) and PE-conjugated anti-CD203c (clone NP4D6, red); merged signal yellow. DNA visualized by DAPI (blue).
Immunocytochemistry/ Immunofluorescence: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, green) and PE-conjugated anti-CD203c (clone NP4D6, ...read more
Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of human peripheral blood by anti-CD203c antibody (NP4D6) FITC.
Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of human basophils in allergen-stimulated whole blood (CD203cpos/ SSClow) by anti-CD203c antibody (NP4D6)
Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of human basophils in IgE-activated whole blood by anti-CD203c antibody (NP4D6) APC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, CyTOF-ready
Clone
NP4D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-44643AF350
Alexa Fluor 405
NBP1-44643AF405
Alexa Fluor 488
NBP1-44643AF488
Alexa Fluor 532
NBP1-44643AF532
Alexa Fluor 594
NBP1-44643AF594
Alexa Fluor 647
NBP1-44643AF647
Alexa Fluor 700
NBP1-44643AF700
Alexa Fluor 750
NBP1-44643AF750
Allophycocyanin
NBP1-44643APC
Allophycocyanin/Cy7
NBP1-44643APCCY7
Biotin
NBP1-44643B
DyLight 350
NBP1-44643UV
DyLight 405
NBP1-44643V
DyLight 488
NBP1-44643G
DyLight 550
NBP1-44643R
DyLight 594
NBP1-44643DL594
DyLight 650
NBP1-44643C
DyLight 680
NBP1-44643FR
DyLight 755
NBP1-44643IR
FITC
NBP1-44643F
HRP
NBP1-44643H
Janelia Fluor 549
NBP1-44643JF549
Janelia Fluor 646
NBP1-44643JF646
PE
NBP1-44643PE
PE/Atto594
NBP1-44643PEATT594
PE/Cy5.5
NBP1-44643PECY55
PE/Cy7
NBP1-44643PECY7
PerCP
NBP1-44643PCP
mFluor Violet 450 SE
NBP1-44643MFV450
mFluor Violet 500 SE
NBP1-44643MFV500
mFluor Violet 610 SE
NBP1-44643MFV610
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ENPP-3/CD203c Antibody (NP4D6) - BSA Free Summary

Additional Information
Clone NP4D6 was used by HLDA to establish CD designation.
Immunogen
HEK-293 cells transfected with human CD203c
Specificity
The mouse monoclonal antibody NP4D6 reacts with CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation. HLDA VIII
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ENPP3
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1 - 5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
Application Notes
This antibody is Cytof ready.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for ENPP-3/CD203c Antibody (NP4D6) - BSA Free

  • B10
  • CD203c antigen
  • CD203c
  • dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3)
  • dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3)
  • ectonucleotide pyrophosphatase/phosphodiesterase 3
  • ectonucleotide pyrophosphatase/phosphodiesterase family member 3
  • E-NPP 3
  • ENPP3
  • ENPP-3
  • gp130RB13-6
  • NPP3
  • PD-IBETA
  • PDNP3
  • PDNP3B10
  • Phosphodiesterase I beta
  • Phosphodiesterase I/nucleotide pyrophosphatase 3
  • phosphodiesterase-I beta

Background

CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ENPP-3/CD203c Antibody (NP4D6) - BSA Free
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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ENPP3