Integrin alpha 2/CD49b Antibody (HAS3) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Feline.

We have publications tested in 3 applications: Flow Cytometry, Functional Assay, Neutralization.

Showing Publications 1 - 7 of 7.

Publications using MAB1233	Applications	Species
Stawikowski M, Aukszi B, Stawikowska R, Cudic M, Fields G Glycosylation modulates melanoma cell alpha2beta1 and alpha3beta1 integrin interactions with type IV collagen. J Biol Chem, 2014-06-23;289(31):21591-604. 2014-06-23 [PMID: 24958723] (Human)		Human
Sophie E. Krieger, Chonsaeng Kim, Lili Zhang, Varpu Marjomaki, Jeffrey M. Bergelson Echovirus 1 Entry into Polarized Caco-2 Cells Depends on Dynamin, Cholesterol, and Cellular Factors Associated with Macropinocytosis Journal of Virology 2013-08-15 [PMID: 23740983]
Londrigan S, Graham K, Takada Y, Halasz P, Coulson B Monkey rotavirus binding to alpha2beta1 integrin requires the alpha2 I domain and is facilitated by the homologous beta1 subunit. J Virol, 2003-09-01;77(17):9486-501. 2003-09-01 [PMID: 12915563] (Neutralization, Human)	Neutralization	Human
Joling P, Broekhuizen R, de Weger R, Rottier P, Egberink H Immunohistochemical demonstration of cellular antigens of the cat defined by anti-human antibodies. Vet Immunol Immunopathol, 1996-09-01;53(1):115-27. 1996-09-01 [PMID: 8941974] (Functional Assay, Feline)	Functional Assay	Feline
Lisignoli G, Monaco M, Facchini A, Toneguzzi S, Cattini L, Hilbert D, Lavaroni S, Belvedere O, Degrassi A In vitro cultured stromal cells from human tonsils display a distinct phenotype and induce B cell adhesion and proliferation. Eur J Immunol, 1996-01-01;26(1):17-27. 1996-01-01 [PMID: 8566062] (Flow Cytometry, Human)	Flow Cytometry	Human
Kim I, Uchiyama H, Chauhan D, Anderson K Cell surface expression and functional significance of adhesion molecules on human myeloma-derived cell lines. Br J Haematol, 1994-07-01;87(3):483-93. 1994-07-01 [PMID: 7993788] (Flow Cytometry, Human)	Flow Cytometry	Human
Kamata T, Takada Y Direct binding of collagen to the I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29) in a divalent cation-independent manner. J Biol Chem, 1994-10-21;269(42):26006-10. 1994-10-21 [PMID: 7523399] (Neutralization, Human)	Neutralization	Human

Array MAB1233

• Integrin alpha 2/CD49b Antibodies
• Integrin alpha 2/CD49b Proteins