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Recombinant Human IL-3 Protein

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Recombinant Human IL-3 Protein (Catalog # 203-IL) has a molecular weight (MW) of 14.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Human IL-3 (Catalog # 203-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.02-0.1 ng/mL.
1 μg/lane of Recombinant Human IL-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-3 Protein Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.1 ng/mL.
Source
E. coli-derived human IL-3 protein
Ala20-Phe152, with and without an N-terminal Met
Accession #
N-terminal Sequence
Ala20 and Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL3
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
203-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-3 Protein

  • Hematopoietic growth factor
  • IL3
  • IL-3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGF
  • MCGFMGC79399
  • MULTI-CSF
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor

Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the  alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

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203-IL
Species: Hu
Applications: Bioactivity

Publications for IL-3 (203-IL)(216)

We have publications tested in 6 confirmed species: Human, Mouse, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Xenograft.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Vivo, SDS-Page.


Filter By Application
Bioassay
(194)
Cell Culture
(17)
Differentiation
(2)
In Vivo
(1)
SDS-Page
(1)
All Applications
Filter By Species
Human
(206)
Mouse
(6)
Porcine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 216. Show All 216 Publications.
Publications using 203-IL Applications Species
Yang, L;Han, Y;Zhang, T;Dong, X;Ge, J;Roy, A;Zhu, J;Lu, T;Vandana, JJ;de Silva, N;Robertson, CC;Xiang, JZ;Pan, C;Sun, Y;Que, J;Evans, T;Liu, C;Wang, W;Naji, A;Parker, SCJ;Schwartz, RE;Chen, S; Human Vascularized Macrophage-Islet Organoids to Model Immune-Mediated Pancreatic ? cell Pyroptosis upon Viral Infection bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39149298] (Bioassay, Human) Bioassay Human
Kent, GM;Atkins, MH;Lung, B;Nikitina, A;Fernandes, IM;Kwan, JJ;Andrews, TS;MacParland, SA;Keller, GM;Gage, BK; Human liver sinusoidal endothelial cells support the development of functional human pluripotent stem cell-derived Kupffer cells Cell reports 2024-08-14 [PMID: 39146183] (Bioassay, Human) Bioassay Human
Abello, J;Yin, Y;Zhao, Y;Maurer, J;Lee, J;Bodell, C;Clevenger, AJ;Burton, Z;Goeckel, ME;Lin, M;Grainger, S;Halabi, CM;Raghavan, SA;Sah, R;Stratman, AN; Endothelial cell Piezo1 promotes vascular smooth muscle cell differentiation on large arteries bioRxiv : the preprint server for biology 2024-06-12 [PMID: 38915529] (Bioassay, Human) Bioassay Human
Landau, S;Zhao, Y;Hamidzada, H;Kent, GM;Okhovatian, S;Lu, RXZ;Liu, C;Wagner, KT;Cheung, K;Shawky, SA;Vosoughi, D;Beroncal, EL;Fernandes, I;Cummins, CL;Andreazza, AC;Keller, GM;Epelman, S;Radisic, M; Primitive macrophages enable long-term vascularization of human heart-on-a-chip platforms Cell stem cell 2024-06-20 [PMID: 38908380] (Bioassay, Human) Bioassay Human
Johnson, D;Colijn, S;Richee, J;Yano, J;Burns, M;Davis, AE;Pham, VN;Saric, A;Jain, A;Yin, Y;Castranova, D;Melani, M;Fujita, M;Grainger, S;Bonifacino, JS;Weinstein, BM;Stratman, AN; Regulation of angiogenesis by endocytic trafficking mediated by cytoplasmic dynein 1 light intermediate chain 1 bioRxiv : the preprint server for biology 2024-04-01 [PMID: 38903077] (Bioassay, Human) Bioassay Human
Paul, SK;Oshima, M;Patil, A;Sone, M;Kato, H;Maezawa, Y;Kaneko, H;Fukuyo, M;Rahmutulla, B;Ouchi, Y;Tsujimura, K;Nakanishi, M;Kaneda, A;Iwama, A;Yokote, K;Eto, K;Takayama, N; Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model Nature communications 2024-06-10 [PMID: 38858384] (Bioassay, Human) Bioassay Human
Payen, SH;Adhikari, K;Petereit, J;Uppal, T;Rossetto, CC;Verma, SC; SARS-CoV-2 superinfection in CD14+ monocytes with latent human cytomegalovirus (HCMV) promotes inflammatory cascade Virus research 2024-04-22 [PMID: 38642618] (Bioassay, Human) Bioassay Human
Capin, J;Harrison, A;Raele, RA;Yadav, SKN;Baiwir, D;Mazzucchelli, G;Quinton, L;Satchwell, TJ;Toye, AM;Schaffitzel, C;Berger, I;Aulicino, F; An engineered baculoviral protein and DNA co-delivery system for CRISPR-based mammalian genome editing Nucleic acids research 2024-02-27 [PMID: 38412306] (Bioassay, Human) Bioassay Human
Freire, CM;King, NR;Dzieciatkowska, M;Stephenson, D;Moura, PL;Dobbe, JGG;Streekstra, GJ;D'Alessandro, A;Toye, AM;Satchwell, TJ; Complete absence of GLUT1 does not impair human terminal erythroid differentiation bioRxiv : the preprint server for biology 2024-01-15 [PMID: 38293086] (Bioassay, Human) Bioassay Human
Wang, P;Zhang, Y;Xiang, R;Yang, J;Xu, Y;Deng, T;Zhou, W;Wang, C;Xiao, X;Wang, S; Foretinib is effective in acute myeloid leukemia by inhibiting FLT3 and overcoming secondary mutations that drive resistance to quizartinib and gilteritinib Cancer research 2024-01-17 [PMID: 38231480] (Cell Culture, Human) Cell Culture Human
Show All 216 Publications.

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Bioinformatics

Gene Symbol IL3
Uniprot