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DOT1L Antibody (S01-7F6)

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Western Blot: DOT1L Antibody (S01-7F6) [NBP3-15025] - Western blot detection of DOT1L in Hela, A549, HL-60 cell lysates using DOT1L Rabbit mAb (1:1000 diluted). Predicted band size: 165kDa. Observed band size: 185kDa.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
S01-7F6
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DOT1L Antibody (S01-7F6) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant protein of human DOT1L (Uniprot#: Q8TEK3)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
DOT1L
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1/1000
Theoretical MW
185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for DOT1L Antibody (S01-7F6)

  • DOT1L
  • DOT1-like protein
  • DOT1-like, histone H3 methyltransferase (S. cerevisiae)
  • EC 2.1.1.43
  • H3-K79-HMTase
  • KIAA1814DKFZp586P1823
  • KMT4
  • KMT4H3 lysine-79 specific
  • Lysine N-methyltransferase 4

Background

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DOT1L Antibody (NBP3-15025) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DOT1L