Western Blot: AF9 Antibody [NBP2-15303] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: AF9 Antibody [NBP2-15303] - AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. ...read more
Immunohistochemistry-Paraffin: AF9 Antibody [NBP2-15303] - Mouse liver. AF9 stained by AF9 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: AF9 Antibody [NBP2-15303] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody diluted at 1:500. ...read more
63 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified
Alternate Names for AF9 Antibody
AF-9
AF9myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 3
ALL1-fused gene from chromosome 9 protein
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 3
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 3
Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein
protein AF-9
YEATS domain-containing protein 3
YEATS3FLJ2035
Background
The human AF9 gene is one of the most common fusion partner genes with the ALL1 gene at 11q23 (also called MLL), resulting in the t(9;11)(p22;q23) fusion. The AF9 gene is more than 100 kb, and 2 patient breakpoint cluster regions (BCRs) have been identified. BCR1 is found within intron 4, previously called site A, whereas BCR2 or site B spans introns 7 and 8.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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