![Western Blot: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of Histone H2B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: PC-12 cell lysate](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Western-Blot-NBP2-67753-img0009.jpg "Western Blot: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of Histone H2B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: PC-12 cell lysate")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | SD20-63 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human Histone H2B aa 110 to the C-terminus (acetyl K121). (SwissProt: O60814 Human; SwissProt: P10854 Mouse; SwissProt: Q64475 Mouse; SwissProt: Q00729 Rat) |
| Localization | Nucleus, Chromosome. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HIST2H2BF |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IP: Use at an assay dependent concentration. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H2B Antibody (NBP2-67753)Find related products by research area.
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| Gene Symbol | HIST2H2BF |
![Immunocytochemistry/Immunofluorescence: Histone H2B Antibody (SD20-63) [NBP2-67753] - Staining Histone H2B in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunocytochemistry-Immunofluorescence-NBP2-67753-img0002.jpg "Immunocytochemistry/Immunofluorescence: Histone H2B Antibody (SD20-63) [NBP2-67753] - Staining Histone H2B in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0008.jpg "Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.")
![Flow Cytometry: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of Hela cells with Histone H2B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Flow-Cytometry-NBP2-67753-img0001.jpg "Flow Cytometry: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of Hela cells with Histone H2B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0003.jpg "Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded human liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0004.jpg "Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded human liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0005.jpg "Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0006.jpg "Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded mouse liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2B-Antibody-SD20-63-Immunohistochemistry-Paraffin-NBP2-67753-img0007.jpg "Immunohistochemistry-Paraffin: Histone H2B Antibody (SD20-63) [NBP2-67753] - Analysis of paraffin-embedded mouse liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.")
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![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_730.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")