Rad9 Antibody (93A535) - BSA Free

Rad9 is a recently isolated human protein that is involved in the control of cell cycle checkpoint. Human Rad9 (hRad9) is homologue of the S. pombe Rad9. Six Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are involved in the early steps of DNA damage check point response in S. pombe. Recently, it has been shown that hRad9 interacts with Bcl-2 family proteins Bcl-2 and Bcl-XL, but not with BAX or BAD. Over-expression of hRad9 in mammalian cells leads to apoptosis, where as antisense Rad9 suppresses cell death induced by DNA damaging agents such as methanosulphonate. Human Rad9 undergoes complex post-translational modifications in undamaged cells and is hyperphosphorylated in response to DNA damage and makes ternary complex with human Rad1 and human HUS1 proteins. This may account for detection of multiple protein bands in some cases by western blot or immunoprecipitation analysis using anti-hRad9 antibody. HRad9 has an intrinsic 3-5 exonuclease activity suggesting that by interaction with DNA it may play a role in the damage-sensing step of the DNA damage checkpoint pathway.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

Showing Publications 1 - 8 of 8.

Publications using NB120-13600	Applications	Species
De Haro LP, Wray J, Williamson EA et al. Metnase promotes restart and repair of stalled and collapsed replication forks. Nucleic Acids Res. 2010-09-01 [PMID: 20457750] (WB) Details: HEk293T cells overexpressing Metnase:1. IP/WB, Fig 6B 2. IP, Fig 6C. The RAD9 IMG-164 mAb (clone 93A535) co-IP'd V5-tagged Metnase. Metnase was detected by WB with a V5 antibody.	WB
Bai H, Madabushi A, Guan X, Lu AL. Interaction between human mismatch repair recognition proteins and checkpoint sensor Rad9-Rad1-Hus1. DNA Repair (Amst). 2010-05-04 [PMID: 20188637] (WB) Details: 1. IF ( HeLaS3, HCT15, LoVo cell lines), Figs 5-7. In MNNG (DNA methylating agent) treated cells RAD9 formed discrete nuclear foci. This is in comparison to unteated cells where RAD9 appeared granulated in faint spots throughout the nucleus. In Fig 7, HeL	WB
Kedar PS, Stefanick DF, Horton JK, Wilson SH. Interaction between PARP-1 and ATR in mouse fibroblasts is blocked by PARP inhibition. DNA Repair (Amst). 2008-11-01 [PMID: 18691676] (WB) Details: WB (M16tsA mouse fibroblasts), Fig 2C. An ATR antibody was used for WB, and the RAD9 IMG-164 antibody was used for WB. The results showed the RAD9 co-IP'd with ATR.	WB
Guan X, Madabushi A, Chang DY et al. The human checkpoint sensor Rad9-Rad1-Hus1 interacts with and stimulates DNA repair enzyme TDG glycosylase. Nucleic Acids Res. 2007-01-01 [PMID: 17855402]
Lee HS, Cho SB, Lee HE et al. Protein expression profiling and molecular classification of gastric cancer by the tissue array method. Clin Cancer Res. 2007-07-15 [PMID: 17634543] Details: Gastric Carcinoma Tissue Microarray (IMH-316): IHC (paraffin), Tables 1, 2; Fig.1. 2. Stomach Tissue Microarray (IMH-341), matching normal tissue of IMH-316: IHC (paraffin), Tables 1, 2; Fig. 1.
Shi G, Chang DY, Cheng CC et al. Physical and functional interactions between MutY glycosylase homologue (MYH) and checkpoint proteins Rad9-Rad1-Hus1. Biochem J. 2006-11-15 [PMID: 16879101] Details: IF (HeLa), Fig 9. In H202 treated HeLa cells Rad9 formed discrete nuclear foci. This is in comparison to untreated HeLa cells where Rad9 appeared granulated in faint spots throughout the nucleus.
Lee HS, Chang MS, Yang HK et al. Epstein-barr virus-positive gastric carcinoma has a distinct protein expression profile in comparison with epstein-barr virus-negative carcinoma. Clin Cancer Res. 2004-03-01 [PMID: 15014022] (IHC-P)	IHC-P
Komatsu K, Wharton W, Hang H et al. PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition. Oncogene. 2000-11-02 [PMID: 11077446] (ICC/IF, Human) Details: IF (Flow2000 human fibroblasts cells), Fig 4.	ICC/IF	Human

Array NB120-13600

• Rad9 Antibodies
• Rad9 Lysate
• Rad9 Proteins