![Western Blot: epithelial Sodium Channel alpha Antibody [NB100-74357] - Analysis of 25 ug of Daudi (lane 1) and mouse brain (lane 2) cell lysates onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Western-Blot-NB100-74357-img0002.jpg "Western Blot: epithelial Sodium Channel alpha Antibody [NB100-74357] - Analysis of 25 ug of Daudi (lane 1) and mouse brain (lane 2) cell lysates onto an SDS polyacrylamide gel.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide corresponding to residues L(20) M K G N K R E E Q G L G P E P A A P Q Q P T(42) C of Human alpha-Enac. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SCNN1A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC/IF uage was reported in scientific literature (PMID: 25953733). WB: Specifically detects an approx. 97 kDa protein representing glycosylated alpha-ENaC from NIH-3T3 cells, and an approx. 75 kDa protein representing the unglycosylated alpha-ENaC protein from human brain samples. |
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| Theoretical MW | 76 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel alpha Antibody [NB100-74357] - performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(tm) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha ENaC Rabbit Polyclonal Antibody at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(tm) Secondary Antibody, Alexa Fluor(R) 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74357-img0004.jpg "Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel alpha Antibody [NB100-74357] - performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(tm) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha ENaC Rabbit Polyclonal Antibody at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(tm) Secondary Antibody, Alexa Fluor(R) 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.")
![Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel alpha Antibody [NB100-74357] - performed using 70% confluent log phase HEK 293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(tm) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha ENaC Rabbit Polyclonal Antibody at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(tm) Secondary Antibody, Alexa Fluor(R) 488 conjugate) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74357-img0003.jpg "Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel alpha Antibody [NB100-74357] - performed using 70% confluent log phase HEK 293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(tm) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha ENaC Rabbit Polyclonal Antibody at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(tm) Secondary Antibody, Alexa Fluor(R) 488 conjugate) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")