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![Western Blot: DOT1L Antibody [NB100-40845] - DOT1L is involved in UV-survival in mammalian cells. Fifty ug of whole cell extracts from MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for DOT1L. Asterisk indicates an unspecific cross-reacting band. Ten ug of fractions from histone acid-extraction performed on MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for H3, H3K79me1 or H3K79me3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003611), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Western-Blot-NB100-40845-img0014.jpg "Western Blot: DOT1L Antibody [NB100-40845] - DOT1L is involved in UV-survival in mammalian cells. Fifty ug of whole cell extracts from MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for DOT1L. Asterisk indicates an unspecific cross-reacting band. Ten ug of fractions from histone acid-extraction performed on MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for H3, H3K79me1 or H3K79me3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003611), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/DOT1L-Antibody-Immunohistochemistry-Paraffin-NB100-40845-img0009.jpg)
![Chromatin Immunoprecipitation: DOT1L Antibody [NB100-40845] - Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of DOT1L binding within a 250 Kb region of chromosome 10 as detected using anti-DOT1L antibody.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Chromatin-Immunoprecipitation-NB100-40845-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: DOT1L Antibody [NB100-40845] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-DOT1L antibody NB100-40845 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B).](http://images.novusbio.com/fullsize/DOT1L-Antibody-Western-Blot-NB100-40845-img0007.jpg)
![Western Blot: DOT1L Antibody [NB100-40845] - siRNA mediated gene silencing of DOT1L validated by Q-PCR and western blot. The results show a down regulation of DOT1L mRNA expression. Empty bars are untreated cells and solid bars are transfected cells. Results from HSkMSC cultures derived from three different experiments on cells at passage three, Graph is presenting means +/- SEM. *, p-value < 0.05 siRNA treated cells compared to controls Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/s12931-017-0525-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Western-Blot-NB100-40845-img0015.jpg)
![Western Blot: DOT1L Antibody (6A6) [H00084444-M01] - DOT1L monoclonal antibody (M01), clone 6A6. Analysis of DOT1L expression in LNCaP.](http://images.novusbio.com/fullsize/DOT1L-Antibody-6A6-Western-Blot-H00084444-M01-img0005.jpg "Western Blot: DOT1L Antibody (6A6) [H00084444-M01] - DOT1L monoclonal antibody (M01), clone 6A6. Analysis of DOT1L expression in LNCaP.")
![Immunocytochemistry/Immunofluorescence: DOT1L Antibody (6A6) [H00084444-M01] - Analysis of monoclonal antibody to DOT1L on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/DOT1L-Antibody-6A6-Immunocytochemistry-Immunofluorescence-H00084444-M01-img0003.jpg)
![Sandwich ELISA: DOT1L Antibody (6A6) [H00084444-M01] - Detection limit for recombinant GST tagged DOT1L is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DOT1L-Antibody-6A6-Sandwich-ELISA-H00084444-M01-img0004.jpg)
![Western Blot: DOT1L Antibody [NBP2-56641] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Western-Blot-NBP2-56641-img0002.jpg "Western Blot: DOT1L Antibody [NBP2-56641] - Analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: DOT1L Antibody [NBP2-56641] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56641-img0001.jpg)
![Western Blot: DOT1L Antibody [NBP2-56641] - Analysis in human cell line BEWO.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Western-Blot-NBP2-56641-img0003.jpg)
![Western Blot: DOT1L Antibody (1D8) [NBP2-46473] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L.](http://images.novusbio.com/fullsize/DOT1L-Antibody-1D8-Western-Blot-NBP2-46473-img0003.jpg "Western Blot: DOT1L Antibody (1D8) [NBP2-46473] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L.")
![Immunohistochemistry: DOT1L Antibody (1D8) [NBP2-46473] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/DOT1L-Antibody-1D8-Immunohistochemistry-NBP2-46473-img0002.jpg)
![Immunohistochemistry: DOT1L Antibody (1D8) [NBP2-46473] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/DOT1L-Antibody-1D8-Immunohistochemistry-NBP2-46473-img0001.jpg)
![Western Blot: DOT1L Antibody (6E7) [NBP2-46474] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L.](http://images.novusbio.com/fullsize/DOT1L-Antibody-6E7-Western-Blot-NBP2-46474-img0003.jpg "Western Blot: DOT1L Antibody (6E7) [NBP2-46474] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L.")
![Immunohistochemistry: DOT1L Antibody (6E7) [NBP2-46474] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DOT1L-Antibody-6E7-Immunohistochemistry-NBP2-46474-img0002.jpg)
![Immunohistochemistry: DOT1L Antibody (6E7) [NBP2-46474] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/DOT1L-Antibody-6E7-Immunohistochemistry-NBP2-46474-img0001.jpg)
![Western Blot: DOT1L Antibody (PSH01-57) [NBP3-32278] - Western blot analysis of DOT1L on different lysates with Rabbit anti-DOT1L antibody (NBP3-32278) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: THP-1 cell lysate Lane 3: HeLa cell lysate Lane 4: 293T cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 165 kDa Observed band size: 180 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32278) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32278_rabbit-dot1l-mab-psh01-57-278202415242613.jpg "Western Blot: DOT1L Antibody (PSH01-57) [NBP3-32278] - Western blot analysis of DOT1L on different lysates with Rabbit anti-DOT1L antibody (NBP3-32278) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: THP-1 cell lysate Lane 3: HeLa cell lysate Lane 4: 293T cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 165 kDa Observed band size: 180 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32278) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DOT1L Antibody (PSH01-57) [NBP3-32278] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DOT1L antibody (NBP3-32278) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32278) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32278_rabbit-dot1l-mab-psh01-57-2982024149264.jpg)
![Western Blot: DOT1L Antibody (7A10H9) [NBP3-15446] - Western blot analysis of extracts of various cell lines, using DOT1L Rabbit mAb (NBP3-15446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/DOT1L-Antibody-7A10H9-Western-Blot-NBP3-15446-img0001.jpg "Western Blot: DOT1L Antibody (7A10H9) [NBP3-15446] - Western blot analysis of extracts of various cell lines, using DOT1L Rabbit mAb (NBP3-15446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunoprecipitation: DOT1L Antibody (7A10H9) [NBP3-15446] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DOT1L antibody (NBP3-15446). Western blot was performed from the immunoprecipitate using DOT1L antibody (NBP3-15446) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/DOT1L-Antibody-7A10H9-Immunoprecipitation-NBP3-15446-img0002.jpg)
![Western Blot: DOT1L Antibody (S01-7F6) [NBP3-15025] - Western blot detection of DOT1L in Hela, A549, HL-60 cell lysates using DOT1L Rabbit mAb (1:1000 diluted). Predicted band size: 165kDa. Observed band size: 185kDa.](http://images.novusbio.com/fullsize/DOT1L-Antibody-S01-7F6-Western-Blot-NBP3-15025-img0001.jpg "Western Blot: DOT1L Antibody (S01-7F6) [NBP3-15025] - Western blot detection of DOT1L in Hela, A549, HL-60 cell lysates using DOT1L Rabbit mAb (1:1000 diluted). Predicted band size: 165kDa. Observed band size: 185kDa.")
![Immunoprecipitation: DOT1L Antibody [NB100-40846] - Detection of Human DOT1L by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody NB100-40846 used for IP at 3 mcg/mg lysate. DOT1L was also immunoprecipitated by rabbit anti- DOT1L antibody NB100-40845, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time 10 seconds.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Immunoprecipitation-NB100-40846-img0002.jpg "Immunoprecipitation: DOT1L Antibody [NB100-40846] - Detection of Human DOT1L by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody NB100-40846 used for IP at 3 mcg/mg lysate. DOT1L was also immunoprecipitated by rabbit anti- DOT1L antibody NB100-40845, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time 10 seconds.")
![Immunocytochemistry/Immunofluorescence: DOT1L Antibody [NBP2-55244] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/DOT1L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55244-img0001.jpg "Immunocytochemistry/Immunofluorescence: DOT1L Antibody [NBP2-55244] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
