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MLLT1 Antibody (3H2)

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Immunocytochemistry/Immunofluorescence: MLLT1 Antibody (3H2) [H00004298-M01] - Analysis of monoclonal antibody to MLLT1 on HeLa cell . Antibody concentration 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: MLLT1 Antibody (3H2) [H00004298-M01] - Analysis of monoclonal antibody to MLLT1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: MLLT1 Antibody (3H2) [H00004298-M01] - Detection limit for recombinant GST tagged MLLT1 is approximately 0.1ng/ml as a capture antibody.
Western Blot: Mouse Monoclonal MLLT1 Antibody (3H2) [H00004298-M01] - Western Blot showing amount of protein, cell lines tested (293T, over expression line 293T+hENL, and mESCs) and loading control blot using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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MLLT1 Antibody (3H2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
MLLT1 (NP_005925, 472 a.a. ~ 558 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GRRSPESCSKPEKILKKGTYDKAYTDELVELHRRLMALRERNVLQQIVNLIEETGHFNVTNTTFDFDLFSLDETTVRKLQSCLEAVA
Specificity
MLLT1 - myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MLLT1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for IF. IHC-P usage reported in scientific literature (PMID: 25481096).
Publications
Read Publication using
H00004298-M01 in the following applications:

  • IHC-P
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25481096). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MLLT1 Antibody (3H2)

  • ENLENL/MLL fusion
  • LTG19MLLT1/MLL fusion
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 1
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 1
  • protein ENL
  • YEATS domain-containing protein 1
  • YEATS1myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 1

Background

MLLT1, also known as ENL (eleven nineteen leukemia protein), was originally identified as a gene involved in a recurrent translocation (11;19) with the mixed-lineage leukemia (MLL) gene. The MLL-ENL fusion protein has been found to associate with SWI/SNF complexes and promote transcription from the HoxA7 promoter. Additionally, from studies to identify proteins that associate with MLLT1, MLLT1 is suggested to play a role in histone modification and transcriptional elongation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MLLT1 Antibody (H00004298-M01)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MLLT1 Antibody (H00004298-M01). (Showing 1 - 1 of 1 FAQ).

  1. I'm attempting to work out conditions for IF using this antibody (H00004298-M01). However, I'm having a hard time finding publications that have published using this specific antibody and the spec sheet does not outline conditions very well. Any and all help is appreciated.
    • This antibody happens to be one that we distribute for a Taiwanese company called Abnova. As such all of the testing is performed in their labs, please see their protocol (http://www.abnova.com/protocol_pdf/Immunofluorescence.pdf). Additionally, this antibody was used at 10ug/mL on HeLa cells.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MLLT1
Entrez
OMIM
Uniprot