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DNA Ligase I Antibody

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Western Blot: DNA Ligase I Antibody [NB100-56635] - Analysis of LIG1 in cell lysate from HeLa cells using this antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DNA Ligase I Antibody Summary

Immunogen
This antibody was developed against a synthetic peptide containing amino acids 51-68 of human LIG1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIG1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Control
HeLa Whole Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.1 mg/ml BSA.
Preservative
0.02% Sodium Azide
Purity
Protein G purified

Alternate Names for DNA Ligase I Antibody

  • DNA ligase 1
  • DNA Ligase I
  • EC 6.5.1.1
  • ligase I, DNA, ATP-dependent
  • MGC117397
  • MGC130025
  • Polydeoxyribonucleotide synthase [ATP] 1
  • polydeoxyribonucleotide synthase ATP 1

Background

Five distinct DNA ligase activities, I-V, have been identified in mammalian cell extracts. One of these enzymes, LIG1 is central to DNA replication. During DNA replication, the joining of Okazaki fragments by the LIG1 gene product appears to be mediated by an interaction with proliferating cell nuclear antigen (PCNA). LIG1 participates in nucleotide excision and base excision repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Research Areas for DNA Ligase I Antibody (NB100-56635)

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Blogs on DNA Ligase I.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and ...  Read full blog post.

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Bioinformatics

Gene Symbol LIG1
Uniprot