Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] diluted at 1:2000. The ...read more
Immunocytochemistry/ Immunofluorescence: DNA Ligase I Antibody (10H5) [NB100-119] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] diluted at ...read more
Immunohistochemistry-Paraffin: DNA Ligase I Antibody (10H5) [NB100-119] - Human breast carcinoma. DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
ICC/IF, IP usage reported in (PMID: 11912211). Use in Radioimmunoassay reported in (PMID: 9603940). Use In vitro assay reported in scientific literature (PMID: 11912211). WB, ICC/IF, IHC-P, IP-Assay dependent.
Theoretical MW
125 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-119 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.. Rabbit reactivity reported in scientific literature (PMID: 12668657). Hamster reactivity reported in scientific literature (PMID: 11912211). Fish, and chicken reactivity reported in scientific literature (PMID: 12928478). Bacteria reactivity reported in scientific literature (PMID: 9603940).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified
Alternate Names for DNA Ligase I Antibody (10H5)
DNA ligase 1
DNA Ligase I
EC 6.5.1.1
ligase I, DNA, ATP-dependent
MGC117397
MGC130025
Polydeoxyribonucleotide synthase [ATP] 1
polydeoxyribonucleotide synthase ATP 1
Background
There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repairand recombination. DNA ligase I migrates as a 125kDa phosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. It also has a role in the base and nucleotide excision repair pathways.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Using PCNA as an Antibody Marker PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and ... Read full blog post.
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![Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/DNA-Ligase-I-Antibody-10H5-Western-Blot-NB100-119-img0003.jpg "Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: DNA Ligase I Antibody (10H5) [NB100-119] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](http://images.novusbio.com/fullsize/DNA-Ligase-I-Antibody-10H5-Immunocytochemistry-Immunofluorescence-NB100-119-img0004.jpg "Immunocytochemistry/Immunofluorescence: DNA Ligase I Antibody (10H5) [NB100-119] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: DNA Ligase I Antibody (10H5) [NB100-119] - Human breast carcinoma. DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/DNA-Ligase-I-Antibody-10H5-Immunohistochemistry-Paraffin-NB100-119-img0001.jpg "Immunohistochemistry-Paraffin: DNA Ligase I Antibody (10H5) [NB100-119] - Human breast carcinoma. DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")